Align Propionyl-CoA carboxylase biotin-containing subunit (EC 6.4.1.3) (characterized)
to candidate WP_011764423.1 AZO_RS03455 acetyl-CoA carboxylase biotin carboxylase subunit
Query= reanno::PS:Dsui_0516 (663 letters) >NCBI__GCF_000061505.1:WP_011764423.1 Length = 668 Score = 1155 bits (2989), Expect = 0.0 Identities = 583/668 (87%), Positives = 618/668 (92%), Gaps = 5/668 (0%) Query: 1 MFKKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALFVEMADEAVCIGPAASKESY 60 MF KILIANRGEIACRVIKTARKMGIKTVAVYSEADKDAL V++ADEAVCIGPA SK+SY Sbjct: 1 MFTKILIANRGEIACRVIKTARKMGIKTVAVYSEADKDALHVDLADEAVCIGPAPSKDSY 60 Query: 61 LVADKIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYSIAKMGDKIESK 120 LV D+IIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYS+AKMGDKIESK Sbjct: 61 LVMDRIIAACKQTGAEAVHPGYGFLSENAEFSRRLEEEGIKFIGPKHYSVAKMGDKIESK 120 Query: 121 KLAIEAKVNTIPGYNDAIDGPDAAVEIAKKIGYPVMIKASAGGGGKGLRVAYNDAEAHEG 180 KLAIEAKVNTIPGYNDAI GP AVEIAKKIGYPVMIKASAGGGGKGLRVAYND +A EG Sbjct: 121 KLAIEAKVNTIPGYNDAIAGPAEAVEIAKKIGYPVMIKASAGGGGKGLRVAYNDEQAFEG 180 Query: 181 FSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIE 240 FSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIE Sbjct: 181 FSSCVNEARNSFGDDRVFIEKYVLEPRHIEIQVLGDSHGNYVYLNERDCSIQRRHQKVIE 240 Query: 241 EAPSPFVDPEMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHPV 300 EAPSPFVD EMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHPV Sbjct: 241 EAPSPFVDAEMRKAMGEQAVALARAVNYESAGTVEFVVSGATKEFYFLEMNTRLQVEHPV 300 Query: 301 TELITGLDLVEQMIRVAYGEKLPLTQADVQINGWAMECRINAEDPFRGFLPSTGRLVKFQ 360 TELITGLDLVEQMIRVA GEKLPL+QADV+INGWAMECRINAEDPFRGFLPSTGRLV+FQ Sbjct: 301 TELITGLDLVEQMIRVAAGEKLPLSQADVKINGWAMECRINAEDPFRGFLPSTGRLVRFQ 360 Query: 361 PPAEVDGQVRVDTGVYDGGEISMYYDSMIAKLIVHGASREQAIARMRDALNGFVIRGISS 420 PPAEVDGQVRVDTGVYDGGEISM+YDSMIAKLIVHGA+R+QAI RMRDALN FVIRGISS Sbjct: 361 PPAEVDGQVRVDTGVYDGGEISMFYDSMIAKLIVHGATRDQAIGRMRDALNAFVIRGISS 420 Query: 421 NIPFQAALMQHARFQSGIFDTGFIAKEYPKGFDASMVPHDDPALLIGVAAFVHRRYIDRA 480 NIPFQAALMQH RF SG F+TGFIA+E+PKGFDASMVPHDDPALL VAA+VHR YIDRA Sbjct: 421 NIPFQAALMQHPRFCSGNFNTGFIAEEFPKGFDASMVPHDDPALLAAVAAYVHRSYIDRA 480 Query: 481 AQVSGQLPGHERKVGDEWVVIRNG-----ERHPVVAKPIEGGYLVTYNGEKYELLSDWRQ 535 A+VSGQLPGHER VG+EWVVIR G E PV A P+ GGY VT NGE Y + SDW+ Sbjct: 481 ARVSGQLPGHERAVGNEWVVIRLGTDNQNELLPVTATPVAGGYAVTLNGETYTIKSDWQL 540 Query: 536 GQSLFNGTCNGEEFTLQVERHRMTYQLFHWGTRADMMVMSARAAELLALMPEKAAPDLSK 595 GQ+LFNGTCNG EFTLQVERH+M Y+LFHWGT+AD +VMS RAAEL +LMP K PDLSK Sbjct: 541 GQALFNGTCNGNEFTLQVERHKMRYRLFHWGTQADFLVMSKRAAELQSLMPVKLPPDLSK 600 Query: 596 FLLSPMPGLLREVSVAVGQEVKAGEKLAVIEAMKMENILKAEQDCKVKKISVTAGSSLSV 655 FLLSPMPGLLREVSV+ GQEVKAGEKLAVIEAMKMEN+LKAEQD KVKK+ + G+SL+V Sbjct: 601 FLLSPMPGLLREVSVSEGQEVKAGEKLAVIEAMKMENLLKAEQDGKVKKVVIKPGASLAV 660 Query: 656 DEIIIEFE 663 D++IIEFE Sbjct: 661 DDVIIEFE 668 Lambda K H 0.319 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1393 Number of extensions: 45 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 663 Length of database: 668 Length adjustment: 38 Effective length of query: 625 Effective length of database: 630 Effective search space: 393750 Effective search space used: 393750 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory