Alignments for a candidate for mcm-large in Azoarcus sp. BH72

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Azoarcus sp. BH72

Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_011764431.1 AZO_RS03495 methylmalonyl-CoA mutase

Query= BRENDA::A4YEG1
         (553 letters)



>NCBI__GCF_000061505.1:WP_011764431.1
          Length = 1088

 Score =  272 bits (696), Expect = 4e-77
 Identities = 195/561 (34%), Positives = 292/561 (52%), Gaps = 41/561 (7%)

Query: 23   KERKNKFT--TPSGIEIKTLYTP-LDLKGD---YEEKIGFPGEYPYTRGIYPNMYRGRIW 76
            KE + K T  + SG +I+ +  P  D  G+   +  K   PG +PYT G++     G   
Sbjct: 531  KEIRTKLTYESLSGSKIRKVALPNFDDDGETLKFLMKENVPGSFPYTAGVFAFKREGEDP 590

Query: 77   TIRQYAGFGSAEDTNARFRKLLEAGQTG-LSTAFDLPTQLGLDPD-NELAYTEVGVVGVS 134
            T R +AG G A  TN RF+K+ E      LSTAFD  T  G DPD     Y ++G  GVS
Sbjct: 591  T-RMFAGEGDAFRTNRRFKKVSEGMPAHRLSTAFDSVTLYGCDPDLRPDIYGKIGNSGVS 649

Query: 135  MFHWKEMDIVTNQIPL--NKVSTSMTINATAMELLSMYVATA---------ESRGVSPTE 183
            +    +M ++ +   L     S SMTIN  A  +L+ +  TA            G  PTE
Sbjct: 650  IATLDDMKVLYDGFDLCCPTTSVSMTINGPAPIILAFFFNTALEQQLAKFKADNGRDPTE 709

Query: 184  -------------IDGTVQNDILKEYIARKNYIYPPEPSMRYAIDIIEYSYKN-IPKWHP 229
                         + GTVQ DILKE   +   I+  E +++   DI E+  +N +  ++ 
Sbjct: 710  DEYAKIRAWTLSSVRGTVQADILKEDQGQNTCIFSTEFALKMMGDIQEFFVRNKVQNFYS 769

Query: 230  ISISGYHIREAGADAVLEVAFTLADGIEYVRRTAERGIPVDDFAPTLSFFFAGYTNLFEE 289
            +SISGYHI EAGA+ + ++AFTLA+G  YV     RG+ +DDFAP LSFFF+   +  E 
Sbjct: 770  VSISGYHIAEAGANPISQLAFTLANGFTYVESYLARGMHIDDFAPNLSFFFSNGMDP-EY 828

Query: 290  VAKFRAARRMWAKIMRDMFNAKKADSMTLKFHTQTGGAELTAQQPEINIIRTTIQALAAA 349
                R ARR+WA  M++ + A +  S  LK+H QT G  L AQ+ + N IRTT+QAL A 
Sbjct: 829  TVIGRVARRIWAVAMKNKYGANER-SQKLKYHVQTSGRSLHAQEMDFNDIRTTLQALIAI 887

Query: 350  LGGTQSLHVNSYDEAVALPSEKAAKIAIRVQQIVAYESGSTETVDPLAGSYYVEWLTDEI 409
                 SLH N+YDEA+  P+E++ + A+ +Q I+  E G  +  +P  GS+ VE LTD +
Sbjct: 888  YDNCNSLHTNAYDEAITTPTEESVRRAMAIQLIINREWGLAKNENPNQGSFIVEELTDLV 947

Query: 410  EERAWKIIERVEGMGGMMKAVERGFPQAEIAESAYRLQKKIEEGEMIRVGVNMSYEPDWI 469
            EE   K  E +   GG++ A+E G+ + +I E +   + K  +G    +GVN    P   
Sbjct: 948  EEAVLKEFEAIASRGGVLGAMETGYQRGKIQEESLYYEHKKHDGSYPIIGVNTFLNPKGQ 1007

Query: 470  GTTEV--FRVNPEIRERVLTRLKKYRSERDQMKVRDSLNALRKAAENPSVNLFPYVLDAI 527
               E+   R   E ++  L RLK + +       +      +   EN   N+F  ++DA+
Sbjct: 1008 AQQEIELARSTEEEKQSQLARLKDFHARNKAEAPKWQAKLQQTVIENG--NVFEVLVDAV 1065

Query: 528  KKGCTVGEISSTLREIWGEYK 548
            +  C++G+I+  L ++ G+Y+
Sbjct: 1066 RY-CSLGQITEALYKVGGQYR 1085


Lambda     K      H
   0.317    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1255
Number of extensions: 56
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 553
Length of database: 1088
Length adjustment: 41
Effective length of query: 512
Effective length of database: 1047
Effective search space:   536064
Effective search space used:   536064
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources