Alignments for a candidate for lctP in Azoarcus sp. BH72

GapMind for catabolism of small carbon sources

Alignments for a candidate for lctP in Azoarcus sp. BH72

Align L-lactate permease (characterized)
to candidate WP_011764898.1 AZO_RS05915 L-lactate permease

Query= SwissProt::P33231
         (551 letters)



>NCBI__GCF_000061505.1:WP_011764898.1
          Length = 548

 Score =  801 bits (2069), Expect = 0.0
 Identities = 398/551 (72%), Positives = 464/551 (84%), Gaps = 3/551 (0%)

Query: 1   MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAASWTVAIALAVALLFYKM 60
           M  W Q YDP GN+WLS+L+A+LPI+FFF AL  L++KG+VA + TVAIALAVA+LFY+M
Sbjct: 1   MQPWMQIYDPLGNLWLSALVAALPIIFFFVALAVLRMKGHVAGTITVAIALAVAILFYQM 60

Query: 61  PVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDIIRSSILSITPDQRLQMLI 120
           PV  ALA+  YGF YGLWPIAWIIIAAVF+YKI+VKTGQFDIIR+S++SIT DQRLQML+
Sbjct: 61  PVPMALAAAGYGFLYGLWPIAWIIIAAVFLYKITVKTGQFDIIRASVVSITDDQRLQMLL 120

Query: 121 VGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLCLIVNTAPVAFGAMGIPILVA 180
           VGF FGAFLEGAAGFGAPVAITAALLVGLGF PLYAAGLCLI NTAPVAFGAMGIPI+V 
Sbjct: 121 VGFSFGAFLEGAAGFGAPVAITAALLVGLGFNPLYAAGLCLIANTAPVAFGAMGIPIIVG 180

Query: 181 GQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWRGIKETWPAVVVAGGSFAIAQYL 240
           GQVTGID F+IGQM GRQLP +++IV FW++A+MDGWRGIKETWPAV+VAGGSFA+ Q+ 
Sbjct: 181 GQVTGIDPFKIGQMAGRQLPLLSMIVPFWLVAMMDGWRGIKETWPAVIVAGGSFAVTQFF 240

Query: 241 SSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFRFGDLGASQVDMTLAHTGYTAGQVL 300
           ++N+IGPELPDI S+LVSL+CLT+FLK W+P  +FRF     +++        Y+ GQ+ 
Sbjct: 241 TANYIGPELPDITSALVSLICLTIFLKNWKPKNIFRFDQQHHAELG---EDRHYSFGQIA 297

Query: 301 RAWTPFLFLTATVTLWSIPPFKALFASGGALYEWVINIPVPYLDKLVARMPPVVSEATAY 360
           +AW+PF+ LTA VT+WS+ PFKALFASGGALY  V   PVP LDKLV +M P+V++AT Y
Sbjct: 298 KAWSPFVILTAIVTVWSLKPFKALFASGGALYSTVFQFPVPMLDKLVIKMEPIVAQATPY 357

Query: 361 AAVFKFDWFSATGTAILFAALLSIVWLKMKPSDAISTFGSTLKELALPIYSIGMVLAFAF 420
           AAV+K D  SATGTAI  +AL+S+V L M+  +A      T+ EL  PIYSIGMVLAFAF
Sbjct: 358 AAVYKLDILSATGTAIFISALVSMVVLGMRGGEAAKALKETVVELKRPIYSIGMVLAFAF 417

Query: 421 ISNYSGLSSTLALALAHTGHAFTFFSPFLGWLGVFLTGSDTSSNALFAALQATAAQQIGV 480
           ++NYSGLS+TLAL LA TG  F FFSPFLGWLGVFLTGSDTSSNALF +LQAT A Q+GV
Sbjct: 418 VANYSGLSATLALLLAGTGALFPFFSPFLGWLGVFLTGSDTSSNALFCSLQATTAHQVGV 477

Query: 481 SDLLLVAANTTGGVTGKMISPQSIAIACAAVGLVGKESDLFRFTVKHSLIFTCIVGVITT 540
           SD LLVAANTTGGVTGKMISPQSIA+ACAAVGLVGKESDLFRFTVKHSL+F  +VGVITT
Sbjct: 478 SDTLLVAANTTGGVTGKMISPQSIAVACAAVGLVGKESDLFRFTVKHSLMFAAMVGVITT 537

Query: 541 LQAYVLTWMIP 551
           LQAYV TWMIP
Sbjct: 538 LQAYVFTWMIP 548


Lambda     K      H
   0.328    0.140    0.436 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1064
Number of extensions: 37
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 551
Length of database: 548
Length adjustment: 36
Effective length of query: 515
Effective length of database: 512
Effective search space:   263680
Effective search space used:   263680
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources