Alignments for a candidate for adh in Azoarcus sp. BH72

GapMind for catabolism of small carbon sources

Alignments for a candidate for adh in Azoarcus sp. BH72

Align Aldehyde dehydrogenase; Acetaldehyde dehydrogenase; EC 1.2.1.3 (characterized)
to candidate WP_011766665.1 AZO_RS14770 aldehyde dehydrogenase family protein

Query= SwissProt::A1B4L2
         (508 letters)



>NCBI__GCF_000061505.1:WP_011766665.1
          Length = 506

 Score =  796 bits (2055), Expect = 0.0
 Identities = 382/492 (77%), Positives = 436/492 (88%)

Query: 17  FEERYDNFIGGEWVAPVSGRYFTNTTPITGAEIGQIARSEAGDIELALDAAHAAKEKWGA 76
           F+ RYDNFIGG+WVAPV G+Y  N TPITG    Q+ARS   DI LALDAAHAA +KWG 
Sbjct: 15  FKSRYDNFIGGKWVAPVRGQYMDNITPITGKPFCQVARSTEEDINLALDAAHAAADKWGR 74

Query: 77  TSPAERANIMLKIADRMERNLELLATAETWDNGKPIRETMAADLPLAIDHFRYFAGVLRA 136
           TS  ERAN + +IADR+E+NLEL+A AET DNGKPIRETMAAD+PLAIDHFRYFAG +RA
Sbjct: 75  TSVIERANTLCRIADRLEQNLELIAYAETVDNGKPIRETMAADIPLAIDHFRYFAGCIRA 134

Query: 137 QEGSISQIDDDTVAYHFHEPLGVVGQIIPWNFPLLMACWKLAPAIAAGNCVVLKPAEQTP 196
           QEGS++++D++TVAYHFHEPLGVVGQIIPWNFP+LMA WKLAPA+AAGN VVLKPAE TP
Sbjct: 135 QEGSLAELDENTVAYHFHEPLGVVGQIIPWNFPILMAAWKLAPALAAGNVVVLKPAESTP 194

Query: 197 AGIMVWANLIGDLLPPGVLNIVNGFGLEAGKPLASSNRIAKIAFTGETTTGRLIMQYASE 256
            GI+V A LI DLLP GVLNIVNG G EAG PLA+S+RIAKIAFTG TTTGRLIMQ AS+
Sbjct: 195 VGILVIAELIADLLPAGVLNIVNGLGREAGVPLATSSRIAKIAFTGSTTTGRLIMQAASQ 254

Query: 257 NLIPVTLELGGKSPNIFFADVAREDDDFFDKALEGFTMFALNQGEVCTCPSRVLIQESIY 316
           N+IPVTLELGGKSPNIFF DVA  DD+FFDKA+EGF +FALNQGEVCTCPSR LI ESIY
Sbjct: 255 NIIPVTLELGGKSPNIFFQDVAAADDEFFDKAVEGFVLFALNQGEVCTCPSRALIHESIY 314

Query: 317 DKFMERAVQRVQAIKQGDPRESDTMIGAQASSEQKEKILSYLDIGKKEGAEVLTGGKAAD 376
           D+FM+RA+ RV+AIKQG+P +++TMIGAQAS+EQ +KILSY+++GK+EGA++L GG  A 
Sbjct: 315 DRFMDRALARVKAIKQGNPLDTETMIGAQASTEQMKKILSYIELGKEEGAQLLAGGGEAK 374

Query: 377 LGGELSGGYYIEPTIFRGNNKMRIFQEEIFGPVVSVTTFKDQAEALEIANDTLYGLGAGV 436
            GGEL+ GYY++PT+F+G+NKMRIFQEEIFGPV+SVTTFKD+AEAL IANDTLYGLGAGV
Sbjct: 375 FGGELAEGYYVQPTVFKGHNKMRIFQEEIFGPVLSVTTFKDEAEALSIANDTLYGLGAGV 434

Query: 437 WSRDANTCYRMGRGIKAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNM 496
           WSRD +T YRMGR IKAGRVWTNCYH YPAHAAFGGYKQSGIGRETHKMMLDHYQQTKN+
Sbjct: 435 WSRDGSTAYRMGRNIKAGRVWTNCYHLYPAHAAFGGYKQSGIGRETHKMMLDHYQQTKNL 494

Query: 497 LVSYSPKKLGFF 508
           LVSYSPK LGFF
Sbjct: 495 LVSYSPKALGFF 506


Lambda     K      H
   0.319    0.136    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 875
Number of extensions: 20
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 506
Length adjustment: 34
Effective length of query: 474
Effective length of database: 472
Effective search space:   223728
Effective search space used:   223728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources