Alignments for a candidate for fadA in Azoarcus sp. BH72

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Azoarcus sp. BH72

Align subunit of β-ketoadipyl CoA thiolase (EC 2.3.1.174; EC 2.3.1.16) (characterized)
to candidate WP_011766784.1 AZO_RS15365 3-oxoadipyl-CoA thiolase

Query= metacyc::MONOMER-3207
         (400 letters)



>NCBI__GCF_000061505.1:WP_011766784.1
          Length = 402

 Score =  575 bits (1481), Expect = e-168
 Identities = 287/398 (72%), Positives = 336/398 (84%), Gaps = 1/398 (0%)

Query: 4   VFICDAIRTPIGRFGGALAGVRADDLAAVPLKALIEPNPAVQWDQVDEVFFGCANQAGED 63
           V++CDAIRTP GR+GG L+ VRADDLAA+P++ALI  NP V W+ VD+V +GCANQAGED
Sbjct: 5   VYLCDAIRTPFGRYGGLLSSVRADDLAALPIRALIARNPGVDWNLVDDVIYGCANQAGED 64

Query: 64  NRNVARMALLLAGLPESIPGVTLNRLCASGMDAIGTAFRAIASGEMELAIAGGVESMSRA 123
           NRNVARMA LLAGLP  +PG T+NRLC S +DAIG+A RAIA+GE EL IAGGVESMSRA
Sbjct: 65  NRNVARMAALLAGLPVEVPGSTVNRLCGSSLDAIGSAARAIAAGEAELMIAGGVESMSRA 124

Query: 124 PFVMGKAESGYSRNMKLEDTTIGWRFINPLMKSQYGVDSMPETADNVADDYQVSRADQDA 183
           PFV+GKA+S +SR+ K+EDTTIGWRF+NPLMK+QYGVDSMPETA+NVA D+ VSRADQDA
Sbjct: 125 PFVLGKADSAFSRSAKIEDTTIGWRFVNPLMKAQYGVDSMPETAENVATDFNVSRADQDA 184

Query: 184 FALRSQQKAAAAQAAGFFAEEIVPVRIAHKKGETI-VERDEHLRPETTLEALTKLKPVNG 242
            ALRSQQ+ A+A   GFFA EIVPV I  KKG+ + +  DEH RP+TTL+ L KLK V  
Sbjct: 185 LALRSQQRWASANERGFFAREIVPVDIPRKKGDPVRMTTDEHPRPDTTLDMLAKLKGVVR 244

Query: 243 PDKTVTAGNASGVNDGAAALILASAEAVKKHGLTPRARVLGMASGGVAPRVMGIGPVPAV 302
           PD TVTAGNASGVNDGAAA++LAS  AVK+HGLTPRAR+LG A+ GVAPR+MGIGP PA 
Sbjct: 245 PDGTVTAGNASGVNDGAAAVLLASEAAVKRHGLTPRARILGSAAAGVAPRIMGIGPAPAS 304

Query: 303 RKLTERLGVAVSDFDVIELNEAFASQGLAVLRELGVADDAPQVNPNGGAIALGHPLGMSG 362
            KL  RLG+ ++ FDVIELNEAFA+Q LAV R LG+ADD  +VNPNGGAI++GHPLG SG
Sbjct: 305 EKLLARLGMGIARFDVIELNEAFAAQALAVTRRLGLADDDARVNPNGGAISIGHPLGASG 364

Query: 363 ARLVLTALHQLEKSGGRKGLATMCVGVGQGLALAIERV 400
           ARLV TAL+QLE SGGR GLATMC+GVGQG+ALAIER+
Sbjct: 365 ARLVTTALNQLETSGGRYGLATMCIGVGQGIALAIERI 402


Lambda     K      H
   0.318    0.134    0.383 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 549
Number of extensions: 14
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 400
Length of database: 402
Length adjustment: 31
Effective length of query: 369
Effective length of database: 371
Effective search space:   136899
Effective search space used:   136899
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_011766784.1 AZO_RS15365 (3-oxoadipyl-CoA thiolase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.10305.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.5e-139  451.2   0.8   1.7e-139  451.0   0.8    1.0  1  lcl|NCBI__GCF_000061505.1:WP_011766784.1  AZO_RS15365 3-oxoadipyl-CoA thio


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000061505.1:WP_011766784.1  AZO_RS15365 3-oxoadipyl-CoA thiolase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  451.0   0.8  1.7e-139  1.7e-139       2     385 .]       8     400 ..       7     400 .. 0.95

  Alignments for each domain:
  == domain 1  score: 451.0 bits;  conditional E-value: 1.7e-139
                                 TIGR01930   2 vdavRtpigklggslkelsaedLlaavikellera.gldpekidevilGnvlqageq.aniaReaalaa 68 
                                                da+Rtp+g++gg l++++a+dL+a  i++l++r+ g+d + +d+vi+G++ qage+  n+aR+aal a
  lcl|NCBI__GCF_000061505.1:WP_011766784.1   8 CDAIRTPFGRYGGLLSSVRADDLAALPIRALIARNpGVDWNLVDDVIYGCANQAGEDnRNVARMAALLA 76 
                                               589******99**********************************************9*********** PP

                                 TIGR01930  69 glpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakle 137
                                               glp +vp+ tvnr+C+S+l+A+ +aa++i+aGea++++aGGvEsmSr+p++l ++   +s+  ++ak e
  lcl|NCBI__GCF_000061505.1:WP_011766784.1  77 GLPVEVPGSTVNRLCGSSLDAIGSAARAIAAGEAELMIAGGVESMSRAPFVLGKA---DSAFSRSAKIE 142
                                               ****************************************************997...45555566666 PP

                                 TIGR01930 138 dqllkdl........vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvev 198
                                               d+++           ++   sm etAen+a+++++sR +qD++alrS+q+ a+A e+g+f++eivpv++
  lcl|NCBI__GCF_000061505.1:WP_011766784.1 143 DTTIGWRfvnplmkaQYGVDSMPETAENVATDFNVSRADQDALALRSQQRWASANERGFFAREIVPVDI 211
                                               644333367888998778889************************************************ PP

                                 TIGR01930 199 kgk...kkvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelglt 264
                                               ++k   ++ +++De++rp+ttl+ LakLk + + ++g tvtAgN+s++nDGAaa+ll+se+++k++glt
  lcl|NCBI__GCF_000061505.1:WP_011766784.1 212 PRKkgdPVRMTTDEHPRPDTTLDMLAKLKGVVR-PDG-TVTAGNASGVNDGAAAVLLASEAAVKRHGLT 278
                                               *999999999**********************9.69*.6****************************** PP

                                 TIGR01930 265 plarivsaavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsld.lekv 332
                                               p ari + a agv p++mg+gp+pA+ek+L++ g+ i  +d++E+nEAFAaq+lav++ lg+ d +++v
  lcl|NCBI__GCF_000061505.1:WP_011766784.1 279 PRARILGSAAAGVAPRIMGIGPAPASEKLLARLGMGIARFDVIELNEAFAAQALAVTRRLGLADdDARV 347
                                               *************************************************************86679*** PP

                                 TIGR01930 333 NvnGGAiAlGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385
                                               N nGGAi++GHPlGasGar+v+t l++L+  g++yGlat+C+g GqG+A+ +e
  lcl|NCBI__GCF_000061505.1:WP_011766784.1 348 NPNGGAISIGHPLGASGARLVTTALNQLETSGGRYGLATMCIGVGQGIALAIE 400
                                               *************************************************9886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (402 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 9.32
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources