Align α-ketoglutaric semialdehyde dehydrogenase subunit (EC 1.2.1.26) (characterized)
to candidate WP_011778304.1 MVAN_RS05210 aldehyde dehydrogenase (NADP(+))
Query= metacyc::G1G01-1343-MONOMER (525 letters) >NCBI__GCF_000015305.1:WP_011778304.1 Length = 532 Score = 637 bits (1644), Expect = 0.0 Identities = 335/520 (64%), Positives = 380/520 (73%), Gaps = 2/520 (0%) Query: 3 LTGNLLIGQRPVTGSRDAIRAIDPTTGQTLEPAYLGGTGEHVAQACALAWAAFDAYRETS 62 LTG +LI PV G+ +RA DP GQ LEP Y G HV ACA A AF YR T+ Sbjct: 11 LTGQMLIAGAPVRGTGKEVRAFDPAAGQPLEPVYQHGDNTHVDAACAAAADAFAQYRATT 70 Query: 63 LEQRAEFLEAIATQIEALGDALIDRAVIETGLPKARIQGERGRTCTQLRTFARTVRAGEW 122 EQRA FL+ IAT IEA+G+ALI RAV E+GLP+ARI GE GRT QLR FA +R G W Sbjct: 71 SEQRAAFLDTIATNIEAVGEALIARAVAESGLPQARITGELGRTTGQLRLFASVLREGSW 130 Query: 123 LDVRIDSALPERQPLPRADLRQRQVALGPVAVFGASNFPLAFSVAGGDTASALAAGCPVV 182 RID+ALP+R PLPR DLRQR + LGPVAVF ASNFPLAFSVAGGDTASALAAGCPVV Sbjct: 131 NGARIDTALPDRTPLPRPDLRQRHIPLGPVAVFSASNFPLAFSVAGGDTASALAAGCPVV 190 Query: 183 VKAHSAHPGTSELVGQAVAQAVKQCGLPEGVFSLLYGSGREVGIALVSDPRIKAVGFTGS 242 VK H AHPGTSELV +AV AV GLP G FSLL+GSG +GIALV+DPRIKAVGFTGS Sbjct: 191 VKGHDAHPGTSELVARAVTDAVTTSGLPAGTFSLLFGSGPGLGIALVTDPRIKAVGFTGS 250 Query: 243 RSGGMALCQAAQARPEPIPVYAEMSSINPVFLFDAALQARAEALAQGFVASLTQGAGQFC 302 RSGGMAL AA ARPEPIPVYAEMSSINPVF+ D AL+ R L + FVASLT G+GQFC Sbjct: 251 RSGGMALVSAAAARPEPIPVYAEMSSINPVFVLDGALKTRGAELGRAFVASLTMGSGQFC 310 Query: 303 TNPGLVIARQGPALQRFITAAAGYVQQGAAQTMLTPGIFSAYQAGIAALADNPHAQAITS 362 TNPGLVIA GP L F AA + A MLTP I +Y +G+ AL+ AQ + Sbjct: 311 TNPGLVIAVDGPGLDTFAAAARDALAGSPATPMLTPTIARSYASGVEALSG--AAQLVGR 368 Query: 363 GQAGQGPNQCQAQLFVTQAEAFLADPALQAEVFGAASLVVACTDDEQVRQVAEHLEGQLT 422 G G C A LF T A+ FLA ALQAEVFG++SL+V C D EQ+R VAE +EGQLT Sbjct: 369 GAPGTSETACHAALFSTDAQTFLASEALQAEVFGSSSLIVRCADFEQMRAVAEGIEGQLT 428 Query: 423 ATLQLDEADIDSARALLPTLERKAGRILVNGWPTGVEVCDAMVHGGPFPATSDARTTSVG 482 AT+ D++D+D A LLP LE KAGRIL GWPTGVEVC AMVHGGPFPATSD+R+TSVG Sbjct: 429 ATVHADDSDLDDAGRLLPLLELKAGRILFGGWPTGVEVCHAMVHGGPFPATSDSRSTSVG 488 Query: 483 TAAILRFLRPVCYQDVPDALLPQALKHGNPLQLRRLLDGK 522 + AI R+LRPVCYQDVP LLP A+ GNP +L R +DG+ Sbjct: 489 SQAIERYLRPVCYQDVPAPLLPSAIAEGNPEKLWRRVDGR 528 Lambda K H 0.319 0.134 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 927 Number of extensions: 31 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 525 Length of database: 532 Length adjustment: 35 Effective length of query: 490 Effective length of database: 497 Effective search space: 243530 Effective search space used: 243530 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory