Alignments for a candidate for Psest_0084 in Mycolicibacterium vanbaalenii PYR-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for Psest_0084 in Mycolicibacterium vanbaalenii PYR-1

Align TRAP transporter, 4TM/12TM fusion protein (characterized, see rationale)
to candidate WP_011779172.1 MVAN_RS09730 C4-dicarboxylate ABC transporter permease

Query= uniprot:E4PQE4
         (729 letters)



>NCBI__GCF_000015305.1:WP_011779172.1
          Length = 677

 Score =  263 bits (672), Expect = 2e-74
 Identities = 166/535 (31%), Positives = 274/535 (51%), Gaps = 20/535 (3%)

Query: 206 PVGISFAAIAGTALIMELTRRVAGMALVIIGLVFLAYVFAGPYLPG--FLGYPGLSVQRF 263
           P+ ++  A+    LI+E  RR  G AL ++  +FLAY + G  +P    + + GL   + 
Sbjct: 144 PIDVAMGALL-LVLILEACRRTTGWALPVVCAMFLAYGYYGGLMPQGWAIAHAGLDFDQI 202

Query: 264 FSQVYTD-AGILGPTTAVSSTYIILFIIFAAFLQSSKVGDYFVNFAFAAAGRSRGGPAKV 322
              +Y   +G  G    V++TYI+LF I+ A L+ S    +FV+ + AA  RSR    + 
Sbjct: 203 IDALYNSGSGFFGTPLDVAATYIVLFTIYGAVLELSGGARFFVDLSVAAFRRSRSAAGRT 262

Query: 323 SIFASGLMGMINGTSAGNVVSTGSLTIPLMKKVGYSKQSAGAVEAAASTGGQIMPPIMGA 382
           ++ +  L+G ++G+     VS G++T P+M++ GY+ + AG V AAA  G  + PP +GA
Sbjct: 263 TVASGFLLGTVSGSGTATAVSVGAITWPIMRRAGYTPERAGGVLAAAGVGALLSPPTLGA 322

Query: 383 GAFIMAEITGIPYTEIAIAAIIPAILYFASVYFMVDFEAAKTGMRGMREDELPKLRTMMK 442
            AFI+AE   + Y  +   A+IP +LY+  +   V+ +A + GM+   +      + + +
Sbjct: 323 AAFIVAEYLEVSYLTVLGWAMIPTVLYYLGILLAVEIDARRFGMKPAHQQSESPWKLLAR 382

Query: 443 QCYLFVPIIILIVALFMGYSVIRAGTLATVSAAVVSWLSP-NKMGLRHILQALEIASYMA 501
             Y F  ++ ++V L  G S  RA   AT  A V+S+L    ++  R +  AL +     
Sbjct: 383 FGYHFSSLVAIVVLLAAGMSATRAVVYATALAFVLSFLDRWGRLTPRRLFDALSVGVRGV 442

Query: 502 IQIIVVCAAAGVIVGVISLTGVGARFSVLL---LDVAATSQLLAL----IFAMFISILLG 554
           + ++ VCAAAGVI  + + TG+GA+FS +L   +D    ++ L L    +FA     LLG
Sbjct: 443 LPVVAVCAAAGVITAMTTKTGLGAQFSSVLVGGVDALTDNRTLMLALTAVFAAVALALLG 502

Query: 555 MGMPTTAAYAVAASVVAPGLVQLGIEPLTAHFFVFYFAVVSAITPPVALASYAAAGISGA 614
           + +P TA++ +   ++ P L+ L +    A  FVFY++V+S +TPP ALA+  A+ ++G 
Sbjct: 503 LAIPVTASFVIGWVIIGPALLALDVPAPAAAMFVFYYSVLSEVTPPTALAAVGASAVTGG 562

Query: 615 NAMETSVASFRIGIAAFIVPFMFFYNGA---LLMEAGWFEIARALVTATFGVYMLSGGVL 671
            A+ T   + R    AF+VP  F        LL       +  A V A  G+  LS    
Sbjct: 563 RAIPTMWQALRYAAPAFLVPIAFVLTAPGEYLLGRGPVLGVIWASVAACAGIVALSFAAG 622

Query: 672 GWFASISA-SWITRLLLIAAALLMIEGGLWTDLTG----IALAVLAFVIQKQRKT 721
           GW   + A + + R     AALL++     T   G    +      F+  K+R+T
Sbjct: 623 GWVLGVGALAPVPRAFTAIAALLLLFLQPATIAAGSACLLGAVAFTFITDKRRRT 677


Lambda     K      H
   0.327    0.139    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1306
Number of extensions: 72
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 729
Length of database: 677
Length adjustment: 39
Effective length of query: 690
Effective length of database: 638
Effective search space:   440220
Effective search space used:   440220
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources