Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_011781246.1 MVAN_RS20505 acetyl-CoA carboxylase subunit beta
Query= metacyc::MONOMER-17283 (535 letters) >NCBI__GCF_000015305.1:WP_011781246.1 Length = 516 Score = 695 bits (1794), Expect = 0.0 Identities = 342/514 (66%), Positives = 401/514 (78%), Gaps = 8/514 (1%) Query: 22 HQSLAADLRERLAQIRQGGGAEQRRRHEERGKLFVRDRIDTLIDPDSSFLEIGALAAYNV 81 H +L LR +LA GG R RH RGKL RDR+D L+DP S FLE+ ALAA + Sbjct: 11 HVALVDQLRTKLAAAALGGPERARERHVSRGKLLPRDRVDGLLDPGSPFLELAALAADGM 70 Query: 82 YDEEVPAAGIVCGIGRVAGRPVMIIANDATVKGGTYFPLTVKKHLRAQEIARENRLPCIY 141 YD+E P AG++ GIGRV+GR MI+ANDATVKGGTY+P+TVKKHLRAQEIA +N+LPCIY Sbjct: 71 YDDECPGAGMIAGIGRVSGRECMIVANDATVKGGTYYPVTVKKHLRAQEIALQNQLPCIY 130 Query: 142 LVDSGGAYLPLQSEVFPDRDHFGRIFYNQAQMSAEGIPQIACVMGSCTAGGAYVPAMSDE 201 LVDSGGA+LP Q EVFPDR+HFGRIFYNQA MSA+GI QIA V+GSCTAGGAYVPAMSDE Sbjct: 131 LVDSGGAFLPRQDEVFPDREHFGRIFYNQATMSAKGIAQIAAVLGSCTAGGAYVPAMSDE 190 Query: 202 VVIVKGNGTIFLGGPPLVKAATGEEVTAEELGGADVHTRISGVADYFANDDREALAIVRD 261 VIV+ GTIFLGGPPLVKAATGE VTAEELGG D+H+++SGV D+ A+DDR+AL IVR Sbjct: 191 AVIVRNQGTIFLGGPPLVKAATGEIVTAEELGGGDLHSKVSGVTDHLAHDDRDALRIVRR 250 Query: 262 IVAHLGPRQRANWELRDPEPPRYDPREIYGILPRDFRQSYDVREVIARIVDGSRLHEFKT 321 IV L P+ WE+ D E+Y ++P D R YDV EVI RIVDG EFK Sbjct: 251 IVGTLAPKADPPWEVAPTVDAVADQAELYDVVPVDSRVPYDVHEVITRIVDGGEFAEFKA 310 Query: 322 RYGTTLVCGFAHIEGFPVGILANNGILFSESALKGAHFIELCCARNIPLVFLQNITGFMV 381 YGTTLV GFA I G PVGI+ANNG+LF ESA+KGAHFIELC R +PL+FLQNI+GFMV Sbjct: 311 EYGTTLVTGFARIHGHPVGIVANNGVLFGESAVKGAHFIELCDKRVVPLLFLQNISGFMV 370 Query: 382 GKQYENGGIAKDGAKLVTAVSCANVPKFTVIIGGSFGAGNYGMCGRAYQPRQLWMWPNAR 441 G+ YE GGIAK GAK+VTAV+CA VPK TV+IGGS+GAGNY MCGRAY PR LWMWPNAR Sbjct: 371 GRDYEAGGIAKHGAKMVTAVACARVPKLTVVIGGSYGAGNYSMCGRAYSPRFLWMWPNAR 430 Query: 442 ISVMGGTQAANVLLTIRRDNLRARGQDMTPEEQERFMAPILAKYEQEGHPYYASARLWDD 501 ISVMGG QAA+VL T+R DMTP+E+E F API A+YE +G+PYY++ARLWDD Sbjct: 431 ISVMGGEQAASVLATVR--------GDMTPDEEEAFKAPIRAQYEDQGNPYYSTARLWDD 482 Query: 502 GVIDPVETRRVLALGLAAAAEAPVQPTRFGVFRM 535 GVIDP +TR V+ L L+ A+AP++P +GVFRM Sbjct: 483 GVIDPADTRTVVGLALSVTAQAPLEPVSYGVFRM 516 Lambda K H 0.322 0.139 0.423 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 981 Number of extensions: 52 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 535 Length of database: 516 Length adjustment: 35 Effective length of query: 500 Effective length of database: 481 Effective search space: 240500 Effective search space used: 240500 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory