Alignments for a candidate for mcm-large in Mycolicibacterium vanbaalenii PYR-1

GapMind for catabolism of small carbon sources

Alignments for a candidate for mcm-large in Mycolicibacterium vanbaalenii PYR-1

Align methylmalonyl-CoA mutase (subunit 2/2) (EC 5.4.99.2) (characterized)
to candidate WP_011781416.1 MVAN_RS21370 methylmalonyl-CoA mutase

Query= BRENDA::O74009
         (563 letters)



>NCBI__GCF_000015305.1:WP_011781416.1
          Length = 529

 Score =  555 bits (1431), Expect = e-162
 Identities = 281/526 (53%), Positives = 378/526 (71%), Gaps = 9/526 (1%)

Query: 40  TDDGFEIKRIYTPADLGEDWNYMEKLGFPGEYPFTRGVYATMYRGRIWTMRQYAGYATAE 99
           T  G  ++ +Y P D   D         PGEYPFTRG +A+ YRG+ WT RQY+G+ TAE
Sbjct: 11  TPSGIPLEPVYGPGDRAADPPP------PGEYPFTRGNFASGYRGKTWTFRQYSGFGTAE 64

Query: 100 ESNKRYKYLLSQGQTGLSVAFDLPTQLGYDSDHPLAEGEVGKVGVAIDSLWDMRILFDGI 159
           ESN+RY+YLL QG TGLSVA DLPTQ GYDSD      EVG+VGVA+D+L D  ILFDGI
Sbjct: 65  ESNRRYRYLLEQGGTGLSVALDLPTQCGYDSDDAEYGEEVGRVGVAVDTLADAEILFDGI 124

Query: 160 PLDKVSTSMTINSTAANLLAMYILVAEEQGVSQEKLRGTVQNDILKEYIARGTYIFPPQP 219
           PLDK+STS TIN TAA LLA Y+  AE++GV +EKL GT+QNDILKEY +RGT+I+PP+P
Sbjct: 125 PLDKISTSFTINGTAAILLAFYVAAAEKKGVPREKLTGTIQNDILKEYASRGTWIWPPEP 184

Query: 220 SMRLTTDIIMYCAENVPKWNPISISGYHIREAGANAVQEVAFTLADGIEYVKAVIERG-M 278
           S+RL  D I +CA  VPK+N IS++G H R+AGANAVQE+AFTLADG+ Y   V+ERG M
Sbjct: 185 SLRLIADTIEFCAAEVPKFNAISVAGAHFRDAGANAVQEMAFTLADGVTYCDTVVERGRM 244

Query: 279 DVDKFAPRLSFFFAAHNNFLEEIAKFRAARRLWAYIMKEWFNAKNPRSMMLRFHTQTAGS 338
            +DKFAP++SFFF  H +F EEIAK+RA RR WA I++E + A   ++ M RF     G+
Sbjct: 245 TIDKFAPQISFFFYTHGDFFEEIAKYRAGRRRWATIVRERYGATTDKASMFRFGCVAGGA 304

Query: 339 TLTAQQPENNIVRVAIQALAAVLGGTQSLHTNSYDEALSLPTEKSVRIALRTQQIIAYES 398
           +L A Q +NN+VRVA +A+AAVLGG QS+ T ++DE  +LP+E+S  +ALRTQQI+AYE+
Sbjct: 305 SLYAPQAQNNLVRVAYEAMAAVLGGVQSMFTAAWDEPFALPSEESATLALRTQQILAYET 364

Query: 399 GVVDTVDPLGGAYYIEWLTDHIYEEALKYIEKIQKMGGMMRAIERGYVQKEIAEAAYKYQ 458
           GV    DPLGG+Y++E LTD   E+ ++ +  ++  GGM+R IE GY+Q  IA+ A+K  
Sbjct: 365 GVTKVADPLGGSYFVEALTDATEEKIIEIMADLEAHGGMVRCIEDGYLQGLIADEAFKIH 424

Query: 459 KEIEEGKRIIVGVNAFVTDE-PIEVEILKVDPSIREKQIERLKKLRSERDNKKVQEALDK 517
           +E+E G R +VGVN FV DE P ++   ++D   R+KQ++RL K++++RD+  V+EAL  
Sbjct: 425 QEVESGVRPVVGVNKFVVDEPPPDLATYELDAEGRDKQLKRLAKVKADRDDVAVKEALAA 484

Query: 518 LRNAAEKEDENLMPYIIEAHRHLATLQEVTDVLREIWGEYRAPLIF 563
           L  AAE  D+NLM  +I+      T+ E+   L+ +WGE++ P++F
Sbjct: 485 LARAAE-GDDNLMHKLIDCANAYCTVGEMVSTLKSVWGEFQQPVVF 529


Lambda     K      H
   0.318    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 782
Number of extensions: 27
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 563
Length of database: 529
Length adjustment: 35
Effective length of query: 528
Effective length of database: 494
Effective search space:   260832
Effective search space used:   260832
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources