Align Propionate--CoA ligase (EC 6.2.1.17) (characterized)
to candidate WP_011781912.1 MVAN_RS23940 propionyl-CoA synthetase
Query= reanno::Phaeo:GFF1175 (629 letters) >NCBI__GCF_000015305.1:WP_011781912.1 Length = 623 Score = 808 bits (2088), Expect = 0.0 Identities = 381/622 (61%), Positives = 471/622 (75%), Gaps = 2/622 (0%) Query: 3 YSEVYEGWKANPEQFWMEAAEAISWDSAPTKALTDKGDGLYEWFADARVNTCYNAVDRHV 62 Y ++++ +P FW AA+A++W P + L D Y WF DA +NTC N +DRHV Sbjct: 4 YRDLFDSSITDPVAFWGAAADAVTWTRPPGRILDDSNPPFYRWFPDAELNTCANTLDRHV 63 Query: 63 EQGRGEQTAIIYDSPITHTKREISYVELRNRVATLAGALRAKGVEKGDRVIIYMPMIPEA 122 + GRG+Q A++YDS +T TKR +Y EL A AGALR GV KGDRV+IYMPMIPEA Sbjct: 64 DAGRGDQPALVYDSAVTDTKRTYTYAELLEATARFAGALRKLGVGKGDRVVIYMPMIPEA 123 Query: 123 LEAMLACARLGAVHSVVFGGFAANELAVRIDDATPKAIIAASCGLEPGRTVHYKPLLDGA 182 + AMLACARLGAVHSVVFGGFA +ELAVRIDD P I++ASCG+EPGR V YKP+LD A Sbjct: 124 VIAMLACARLGAVHSVVFGGFAPHELAVRIDDVRPTVIVSASCGIEPGRVVEYKPMLDAA 183 Query: 183 IDLATHKPDFCVIFQREQEVAELIEGRDVNWHGFQYGVEPAECVPVEGNHPAYILYTSGT 242 + + H P CV+ QR++ +L+EGRD++W G EP + VPV P Y+LYTSGT Sbjct: 184 VGMVEHPPRHCVVVQRDRHRCDLVEGRDLDWADVMAGAEPVDPVPVAATDPLYVLYTSGT 243 Query: 243 TGQPKGVIRHTAGQLVALNWTMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHGNTT 302 TG+PKG++R G VAL W+M++IY+++PGDVFWAASDVGWVVGHSYI Y PL+ G TT Sbjct: 244 TGKPKGIVRDNGGHAVALLWSMRHIYDIEPGDVFWAASDVGWVVGHSYIVYAPLLLGATT 303 Query: 303 IVFEGKPIGTPDAGTFWRVISEHKVKSFFTAPTAFRAVKREDPKGEFVKKYDLSCLKQVY 362 +++EGKP+GTPD G FWRV SEH VK+ FTAPTA RA+++EDP+G++V YDLS LK ++ Sbjct: 304 VLYEGKPVGTPDPGAFWRVASEHGVKALFTAPTAIRAIRKEDPEGKYVGDYDLSGLKYLF 363 Query: 363 LAGERADPDTITWAQEQLKVPVIDHWWQTETGWSIAANPLGIEELPTKLGSPAVPMPGYT 422 LAGER DPDT WA E+L +PVIDHWWQTETGW+IAANP+G E LP K GSP VPMPGY Sbjct: 364 LAGERLDPDTYHWASEKLGIPVIDHWWQTETGWAIAANPMGTEALPLKAGSPTVPMPGYD 423 Query: 423 VDILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEDRFKKSYLTTFPGYYETGDAGMK 482 V IL + GH G+ GAI ++LPLPPGTLPTLWNA+DR++ SYLT PGYY TGD G Sbjct: 424 VRILHDHGHECETGQEGAICIRLPLPPGTLPTLWNADDRYQASYLTEHPGYYLTGDGGRF 483 Query: 483 DEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVSDSLKGQAPVGFLC 542 DEDGYL++M R DDVINVAGHRLSTGA+EEVLA HP VAECAVIGV+D +KGQAP G + Sbjct: 484 DEDGYLFVMGRIDDVINVAGHRLSTGAIEEVLATHPAVAECAVIGVADEIKGQAPRGLVV 543 Query: 543 LNAGCDTPHEDVVAQVVKLVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVNIADGTD 602 + AG T + + ++VKLVR++IG VA+FKL VV LPKTRSGKILR TM IA G D Sbjct: 544 VKAGAST--DGLADELVKLVRDEIGAVASFKLVDVVPALPKTRSGKILRKTMRGIAAGRD 601 Query: 603 WKMPATIDDPAILDEITTALQG 624 +P+TI+DPA+LD ++ L+G Sbjct: 602 EPIPSTIEDPAVLDALSPILRG 623 Lambda K H 0.318 0.137 0.428 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1407 Number of extensions: 52 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 629 Length of database: 623 Length adjustment: 38 Effective length of query: 591 Effective length of database: 585 Effective search space: 345735 Effective search space used: 345735 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory