Align protein-Npi-phosphohistidine-D-mannitol phosphotransferase (EC 2.7.1.197) (characterized)
to candidate WP_011782706.1 MVAN_RS28100 PTS mannitol transporter subunit IICBA
Query= BRENDA::P00550 (637 letters) >NCBI__GCF_000015305.1:WP_011782706.1 Length = 658 Score = 623 bits (1607), Expect = 0.0 Identities = 329/649 (50%), Positives = 431/649 (66%), Gaps = 30/649 (4%) Query: 5 IKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWL---------PNETLAKL---- 51 ++++VQ G LSNMVMPNIGAFIAWG+ITALFI GWL P+ +AK+ Sbjct: 15 VRVRVQKLGTALSNMVMPNIGAFIAWGLITALFIEQGWLQGIFAELKNPDGWVAKIGGWG 74 Query: 52 -------VGPMITYLLPLLIGYTGGKLVGGERGGVVGAITTMGVIVGADMPMFLGSMIAG 104 VGPMITYLLP+LIGYTGG++V G RG VVGAI TMGV+ GAD+PMFLG+MI G Sbjct: 75 AYDGAGIVGPMITYLLPVLIGYTGGRMVHGNRGAVVGAIATMGVVTGADVPMFLGAMIMG 134 Query: 105 PLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKMLAAG 164 PLGGWC+K D +GKI+ GFEMLV+NFSAGI+G+ILA+ F GIGPIV + ++ Sbjct: 135 PLGGWCMKKLDALWEGKIRPGFEMLVDNFSAGILGLILAVFGFFGIGPIVSSFTRAAGNA 194 Query: 165 VNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIEANPGPG 224 V+F+V +D+LPL SI +EPAK+LFLNNAINHG+ +PLG Q+ E GKSI FL+EANPGPG Sbjct: 195 VDFLVENDLLPLTSILIEPAKVLFLNNAINHGVLTPLGTTQALETGKSILFLLEANPGPG 254 Query: 225 MGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILGGMTGVFTL 284 +G+LLAYM FGRG A+ SA GAAII F GGIHEIYFPYVLM P+LI+A ILGGMTGVF Sbjct: 255 LGLLLAYMAFGRGLARASAPGAAIIQFFGGIHEIYFPYVLMKPKLIVATILGGMTGVFIN 314 Query: 285 TILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILLKTSKVKEEDD 344 + G GL +PA+PGSI+AV A T G++ V + AVSF V+A+LLKT + +E D Sbjct: 315 VLFGSGLRAPAAPGSIIAVYAQTASGSFLGVTLSVFGSAAVSFAVAALLLKTDRATDESD 374 Query: 345 IEAATRRMQDMKAESKGASPLSAGDVTNDLSHVRKIIVACDAGMGSSAMGAGVLRKKIQD 404 + AAT M+ +K + S ++ V +R I+ ACDAGMGSSAMGA VLR+KIQ Sbjct: 375 LAAATAEMESLKGKK---SSVAGALVATSTGPIRSIVFACDAGMGSSAMGASVLRRKIQQ 431 Query: 405 AGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLTNFLDSGLYTSLTER 464 AG + VTNSAI+NL DLVI+HRDLT RA ++ A H+S+ +F+ S Y + ER Sbjct: 432 AGFGDVKVTNSAISNLSDTYDLVISHRDLTARARQRTGSAVHVSVDDFMGSPRYDEIVER 491 Query: 465 LVAAQRHTANEEKVKDSLKDSFDDSSANLFKLGAENIFLGRKAATKEEAIRFAGEQLVKG 524 L Q +T + ++++ + + ++ L +I L A T +AI AG LV Sbjct: 492 L--KQTNTGGGDGAPAAVEEPAEAPADDVLPL--SSIVLDGAATTAADAITEAGRLLVTA 547 Query: 525 GYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKTGVVFCQYPEGVRFGEEED 584 G VEP Y+ AM RE TY+G +A+PHGT EAKD + +TG+ F +Y E + + + Sbjct: 548 GAVEPAYIDAMHQRESSISTYMGNGLAIPHGTNEAKDTIRRTGLSFVRYAEPIDWNGKP- 606 Query: 585 DIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTTSVDEVLELL 633 A V+GIA +H+ ++T + + RL T+ +EV +L Sbjct: 607 --AEFVVGIAGAGKDHMALLTKIAQVFLKADDVARLREATTTEEVKAIL 653 Lambda K H 0.321 0.138 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 983 Number of extensions: 47 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 637 Length of database: 658 Length adjustment: 38 Effective length of query: 599 Effective length of database: 620 Effective search space: 371380 Effective search space used: 371380 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory