Align Putative alpha-glucosidase AglA (characterized, see rationale)
to candidate WP_011840128.1 RSPH17029_RS00200 DUF3459 domain-containing protein
Query= uniprot:I7EUW4 (552 letters) >NCBI__GCF_000015985.1:WP_011840128.1 Length = 536 Score = 596 bits (1536), Expect = e-175 Identities = 285/502 (56%), Positives = 352/502 (70%), Gaps = 9/502 (1%) Query: 18 DWWRGAVIYQIYPRSYQDSNGDGIGDLRGITQRLPHIASLGVDAIWISPFFTSPMKDFGY 77 DWWRG+V YQIYPRS+QDS+GDG+GDLRGI +RL H+A LG DAIW+SP F SPM+D GY Sbjct: 3 DWWRGSVTYQIYPRSFQDSDGDGVGDLRGIIERLDHVAWLGADAIWLSPIFPSPMEDMGY 62 Query: 78 DVSDYCDVDPMFGSLSNFDQLVAAAHRLGLRVMIDLVLSHTSDQHAWFGESRQSRDNARA 137 DVSDY + P+FGS+ +FD L+A AH LGL+V+ID VLSH+S H +F ESR+SRDN +A Sbjct: 63 DVSDYTAIHPLFGSMEDFDALLARAHELGLKVIIDQVLSHSSSAHPFFRESRRSRDNPKA 122 Query: 138 DWYVWADPQPDGTPPNNWLSIFGGSAWQWDPRREQYYLHNFLVSQPDLNFHSPAVQDALL 197 DWYVWAD QPDG+PPNNWLS+FGGSAW+WD +R QYYLHNFL+SQPD NFH+P VQD LL Sbjct: 123 DWYVWADAQPDGSPPNNWLSVFGGSAWEWDAQRRQYYLHNFLISQPDFNFHNPEVQDWLL 182 Query: 198 DVTRFWLERGVDGFRLDTINFYYHDAELRSNPALPPEQRNATIAPSVNPYNHQEHLYSKN 257 D RFWL+RGVDGFRLDT+NFY+HDAELRSN P +N I P VN Y+ Q+H +SK+ Sbjct: 183 DQMRFWLDRGVDGFRLDTVNFYFHDAELRSN---RPNPQNGPI-PPVNAYDMQDHAFSKS 238 Query: 258 QPENLAFLGRFRALLDEYPAKAAVGEVGDAQRGLEIMGSYTAANTGVHMCYAFELLAKDV 317 + EN+AFL R R LLD+YP +A VGEV D RGL IM YT+ +HM Y FE+L++ Sbjct: 239 RIENIAFLLRMRKLLDDYPDRAMVGEVADGLRGLAIMAEYTSGADRLHMAYTFEMLSR-T 297 Query: 318 LTASRLAEVFAEVDRVAANGWACWAFSNHDVIRHSSRW---GLNPAAQRLFTTMMMCLRG 374 TA E A +GW CWAFSNHDV+RH++RW G RL M++ G Sbjct: 298 FTAGHFRSRIEEFFATAPHGWPCWAFSNHDVVRHATRWAGHGAEDDVARLAAAMLLSFEG 357 Query: 375 TTCIYQGEELGLPEADIAFEDLQDPYGIEFWPEFKGRDGCRTPMVWEPSNGSGGFSAGKP 434 + +YQGEELG E ++ +E+L DP G+ FWPE KGRDGCRTPMVW+ +GGF+ G P Sbjct: 358 SVSLYQGEELGQTETELLYEELTDPPGLRFWPEEKGRDGCRTPMVWD-DGPAGGFTTGTP 416 Query: 435 WLPVSPEHLNLSVASQEADPDAMLHHYRRAIALRKAHPALAVGTHDQLRAEGNVAFFTRQ 494 WLPV P L +VASQ+ P ++L YR + R+ PAL G + FTR Sbjct: 417 WLPVKPPQLARNVASQKGIPGSVLETYRALLQFRRTQPALIRGRSRFFDLPEPMLGFTRT 476 Query: 495 DRDEVIFCAFNLGDIPAEITLP 516 + + C FNLG P TLP Sbjct: 477 LDGQSLACFFNLGIEPVSATLP 498 Lambda K H 0.321 0.137 0.444 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1071 Number of extensions: 60 Number of successful extensions: 6 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 552 Length of database: 536 Length adjustment: 35 Effective length of query: 517 Effective length of database: 501 Effective search space: 259017 Effective search space used: 259017 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory