Alignments for a candidate for acdH in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for acdH in Cereibacter sphaeroides ATCC 17029

Align 2-methylbutanoyl-CoA dehydrogenase (EC 1.3.8.5) (characterized)
to candidate WP_011840308.1 RSPH17029_RS01585 acyl-CoA dehydrogenase family protein

Query= reanno::pseudo6_N2E2:Pf6N2E2_1146
         (375 letters)



>NCBI__GCF_000015985.1:WP_011840308.1
          Length = 555

 Score =  233 bits (595), Expect = 7e-66
 Identities = 144/383 (37%), Positives = 210/383 (54%), Gaps = 14/383 (3%)

Query: 5   EEQTQIRDMARQFAEERLKPFAAEWD-REHRFPREAIDEMAELGFFGMLVPEQWGGCDTG 63
           EE   IRD  R+FA+ER+ P A  W  R+   P E ++ +AE+G FG+ +PE++GG    
Sbjct: 173 EELEMIRDQFRRFADERVAPHAHGWHMRDELIPMEIVEALAEMGVFGLTIPEEFGGFGLS 232

Query: 64  YLAYAMTLEEIAAGDGACSTIMSVHNSVGCVPILKFGNDEQKAKFLTPLASGAMLGAFAL 123
             +  +  EE++ G     ++    + +    IL  G D QKA +L  LASG +L     
Sbjct: 233 KASMVVVSEELSRGYIGVGSL-GTRSEIAAELILCGGTDAQKAAWLPKLASGEILPTAVF 291

Query: 124 TEPQAGSDASSLKTRARLEGDHYVLNGCKQFITSGQNAGVVIVFAVTDP-SAGKRGISAF 182
           TEP  GSD  SL+TRA  +GD +V+NG K +IT      V+ + A TDP +   RG+S F
Sbjct: 292 TEPNTGSDLGSLRTRAVKDGDEWVVNGNKTWITHAARTHVMTLLARTDPETTDYRGLSMF 351

Query: 183 IV---------PTDSPGYSVARVEDKLGQHASDTCQILFEDLKVPVGNRLGE-EGEGYKI 232
           +          P  +PG +   +E  LG       +I F+  +V   N LG  EG+G+K 
Sbjct: 352 LAEKMPGTDADPFPTPGMTGGEIE-VLGYRGMKEYEIGFDGFRVKGENLLGGVEGQGFKQ 410

Query: 233 ALANLEGGRVGIAAQAVGMARAAFEAARDYARERSSFGKPIIEHQAVAFRLADMATQIAV 292
            +   E  R+  AA+A+G+A+ A E    YA ER  FGK +IE   VA +LA MA +I V
Sbjct: 411 LMQTFESARIQTAARAIGVAQNALEVGMQYAEERKQFGKALIEFPRVAGKLAMMAVEIMV 470

Query: 293 ARQMVHYAAALRDSGQPALVEASMAKLFASEMAEKVCSMALQTLGGYGYLNDFPLERIYR 352
           ARQ+ +++A  +D GQ   +EA MAKL  + +A      ALQ  GG G+  ++ + RI  
Sbjct: 471 ARQLTYHSAWEKDHGQRCDLEAGMAKLLGARVAWAAADNALQIHGGNGFALEYQISRILC 530

Query: 353 DVRVCQIYEGTSDIQRMVISRNL 375
           D R+  I+EG ++IQ  VI+R L
Sbjct: 531 DARILNIFEGAAEIQAQVIARRL 553



 Score = 34.7 bits (78), Expect = 7e-06
 Identities = 27/97 (27%), Positives = 45/97 (46%), Gaps = 5/97 (5%)

Query: 237 LEGGRVGIAAQAVGMARAAFEAARDYARERSSF-----GKPIIEHQAVAFRLADMATQIA 291
           L G  + +A+ A+    A FE AR   +ER +       K + E Q  A  L+ +AT + 
Sbjct: 13  LLGDLLTLASDALPEVEALFETARSALKERVTTDGKVSSKALEEEQFAAHALSWLATYVE 72

Query: 292 VARQMVHYAAALRDSGQPALVEASMAKLFASEMAEKV 328
             RQM  +A  L   G+   +EA + ++   E   ++
Sbjct: 73  SLRQMRAWAGRLETEGRFGEMEALILQIAFGEYLAQI 109


Lambda     K      H
   0.320    0.134    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 474
Number of extensions: 27
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 375
Length of database: 555
Length adjustment: 33
Effective length of query: 342
Effective length of database: 522
Effective search space:   178524
Effective search space used:   178524
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources