Align L-glutamate gamma-semialdehyde dehydrogenase (EC 1.2.1.88); Proline dehydrogenase (EC 1.5.5.2) (characterized)
to candidate WP_011840638.1 RSPH17029_RS04265 bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA
Query= reanno::Phaeo:GFF1160 (1158 letters) >NCBI__GCF_000015985.1:WP_011840638.1 Length = 1146 Score = 1408 bits (3645), Expect = 0.0 Identities = 759/1138 (66%), Positives = 876/1138 (76%), Gaps = 29/1138 (2%) Query: 34 VDQAQMRDQLFALANLDATDRSTISANAAALVRDIRGHSSPGLMEVFLAEYGLSTDEGVA 93 +D+A + +L A A+LD + R+ I A A LVR IR S PGLME FLAEYGLSTDEG+A Sbjct: 14 LDEAGLVARLIAEADLDPSARARIVARGADLVRRIRQGSRPGLMEGFLAEYGLSTDEGIA 73 Query: 94 LMCLAEALLRVPDADTIDALIEDKIAPSEWGKHLGKSTSSLVNASTWALMLTGKVLDEKR 153 LMCLAEALLRVPDA+T+DALIEDKIAPS+W +HLG+S SSLVNASTWALMLTGKVLD++ Sbjct: 74 LMCLAEALLRVPDAETMDALIEDKIAPSDWNRHLGRSASSLVNASTWALMLTGKVLDDRE 133 Query: 154 SPVSA-LRGAMKRLGEPVIRTAVSRAMKEMGRQFVLGETIEGAMKRAAGMEAKGYTYSYD 212 V+ LRG ++RLGEPVIR AV+RAMKEMGRQFVLGETIEGAM+RAA +EAKGY+YSYD Sbjct: 134 PGVAGHLRGLVRRLGEPVIRKAVARAMKEMGRQFVLGETIEGAMERAAELEAKGYSYSYD 193 Query: 213 MLGEAARTEADAARYHLAYSRAISAIAAACNSADIRQNPGISVKLSALHPRYELAQETSV 272 MLGEAARTEADA RYHLAYSRAI+AIAAA S DIR NPGISVKLSALHPRYE+A+ V Sbjct: 194 MLGEAARTEADARRYHLAYSRAITAIAAAAKSRDIRDNPGISVKLSALHPRYEVAKRARV 253 Query: 273 KEQLVPRLQALALLAKAAGMGLNVDAEEADRLSLSLEVIEEVISDPALAGWDGFGVVVQA 332 +LVPR+ ALA LA+AAG+G N+DAEEADRL LSLEVI +++P+LAGWDGFGVVVQA Sbjct: 254 LAELVPRVTALAGLARAAGLGFNIDAEEADRLDLSLEVIAAALAEPSLAGWDGFGVVVQA 313 Query: 333 YGPRTGAALDALYDMANRYDRRLMVRLVKGAYWDTEVKRAQVEGVDGFPVFTHKSLTDVS 392 YG R G LD LY MA R DR+LMVRLVKGAYWD EVKRAQV G+ FPVFT K TDVS Sbjct: 314 YGRRAGEVLDRLYGMAVRLDRKLMVRLVKGAYWDGEVKRAQVLGLADFPVFTRKMATDVS 373 Query: 393 YIANARKLLSITDRIYPQFATHNAHTVSAILHMAKDTDKGAYEFQRLHGMGETLHNMVLE 452 YIA ARKL S++DRIYPQFATHNAHTV+AIL +A+ +EFQRLHGMGE LH++VL Sbjct: 374 YIAQARKLFSMSDRIYPQFATHNAHTVAAILELAEGR---PFEFQRLHGMGERLHDLVLT 430 Query: 453 QNQTHCRIYAPVGAHRDLLAYLVRRLLENGANSSFVNQIVDENVPPELVAADPFAQVEDL 512 + +T CRIYAPVGAHRDLLAYLVRRLLENGANSSFVNQIVDE+V PE VAA P +E Sbjct: 431 EEKTRCRIYAPVGAHRDLLAYLVRRLLENGANSSFVNQIVDESVSPEAVAACPLTAMERT 490 Query: 513 ----TANLRKGPDLFQPERPNSIGFDLGHAPTLAAIDAARAPWKSHSWAAEPLLAKAPET 568 LR G DLF P R NS GFDL A LA I++AR+P ++ + A P+LA++ Sbjct: 491 EPVRNPVLRAGTDLF-PGRRNSRGFDLAAAEDLALIESARSPHRTALFDARPILAES--L 547 Query: 569 ATTTDEPVRNPADLTTVGRVQTAGQAEIETALSAATPWNASAETRAEVLNRAADLYEANY 628 A V +PA VG V A ++ETAL A PW A E RAEVL RAADLYEA++ Sbjct: 548 AGGQRAEVTDPATGARVGNVLPASLPDVETALHHAVPWEARPEDRAEVLRRAADLYEASF 607 Query: 629 GELFALLTREAGKTLPDCVAELREAVDFLRYYAARISA--EPPVGVFTCISPWNFPLAIF 686 G +FALL REAGKTLPD V ELREAVDFLRYYAA A PP+G CISPWNFPLAIF Sbjct: 608 GPIFALLAREAGKTLPDAVGELREAVDFLRYYAAEGEALTAPPLGTVVCISPWNFPLAIF 667 Query: 687 SGQIAAALAVGNAVLAKPAEQTPLIAHRAISLLHEAGVPRSALQLLPG-AGAVGGALTSD 745 +GQIAAAL GNAVLAKPAEQTPLIA A+ LLH AGVP +ALQLLPG G VG ALT D Sbjct: 668 TGQIAAALMAGNAVLAKPAEQTPLIAALAVELLHRAGVPATALQLLPGEGGTVGAALTRD 727 Query: 746 ARVGGVAFTGSTATALKIRAAMAEHLRPGAPLIAETGGLNAMIVDSTALPEQAVQSIIES 805 RVGGV FTGST TA IR +MA+HL P APLIAETGGLNAMIVDSTALPEQAV+ I+ S Sbjct: 728 PRVGGVVFTGSTETARAIRQSMADHLDPAAPLIAETGGLNAMIVDSTALPEQAVRDILAS 787 Query: 806 AFQSAGQRCSALRCLYLQEDIADNVLKMLKGAMDALHLGDPWNLSTDSGPVIDETARAGI 865 AFQSAGQRCSALRCLYLQED+A++VL+ML GAMD L G+PW+L+TD GPVID A+AGI Sbjct: 788 AFQSAGQRCSALRCLYLQEDVAESVLEMLCGAMDELQAGEPWDLATDLGPVIDAEAQAGI 847 Query: 866 LAHIDAARAEGRVLKEMTAPQGGTFVAPTLIEITGIQALEQEIFGPVLHVVRFKSQDLDQ 925 I AARAEGRVLK+M P GTFV PT+I + I+ +++EIFGPVLHV F++ ++D+ Sbjct: 848 RDWIAAARAEGRVLKQMAVPARGTFVGPTVIRVGSIREMDREIFGPVLHVATFRATEIDR 907 Query: 926 IIRDINATGYGLTFGLHTRIDDRVQYICDRIHAGNLYVNRNQIGAIVGSQPFGGEGLSGT 985 +I +I+ATGYGLTFGLH+RIDDRVQ + R+ GN+YVNRNQIGA+VGSQPFGGEGLSGT Sbjct: 908 VIAEIDATGYGLTFGLHSRIDDRVQALVGRLRVGNIYVNRNQIGAVVGSQPFGGEGLSGT 967 Query: 986 GPKAGGPFYMMRFCAPDRQKSVDSWPSDAP---------AMTMLPAPTGQPMQEITTSLP 1036 GPKAGGP Y+ R + + + D S+AP A+ L G+ + T +LP Sbjct: 968 GPKAGGPAYLARLVS---RPAPDCAGSEAPAQPAAEVQAALDRLAPKAGELIG--TRALP 1022 Query: 1037 GPTGESNRLSQLARPPLLCLGPGPQAVVAQARAVHALGGTAIEATGPLDMRQLLTMEGTS 1096 GPTGESNRLS L RPPLLCLGPGP A AQA AV ALGG A+EA G L L T+ G + Sbjct: 1023 GPTGESNRLSLLRRPPLLCLGPGPAAARAQAEAVQALGGLAVEAPG-LAPEALATLRGFA 1081 Query: 1097 GVIWWGDETTAREIESWLARRNGPILPLIPGLPDKARVQAERHVCVDTTAAGGNAALL 1154 G ++WG AR LA R G ILPLI GLPD+A V ERH+C+DTTA+GGNA LL Sbjct: 1082 GALYWGGAGDARPRAQALASREGAILPLIGGLPDEAHVAVERHLCIDTTASGGNAELL 1139 Lambda K H 0.317 0.132 0.387 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 3159 Number of extensions: 147 Number of successful extensions: 11 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 1158 Length of database: 1146 Length adjustment: 47 Effective length of query: 1111 Effective length of database: 1099 Effective search space: 1220989 Effective search space used: 1220989 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)
Align candidate WP_011840638.1 RSPH17029_RS04265 (bifunctional proline dehydrogenase/L-glutamate gamma-semialdehyde dehydrogenase PutA)
to HMM TIGR01238 (delta-1-pyrroline-5-carboxylate dehydrogenase (EC 1.2.1.88))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01238.hmm # target sequence database: /tmp/gapView.452784.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01238 [M=500] Accession: TIGR01238 Description: D1pyr5carbox3: delta-1-pyrroline-5-carboxylate dehydrogenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 5.8e-188 611.4 0.1 1.1e-187 610.5 0.1 1.4 1 NCBI__GCF_000015985.1:WP_011840638.1 Domain annotation for each sequence (and alignments): >> NCBI__GCF_000015985.1:WP_011840638.1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 610.5 0.1 1.1e-187 1.1e-187 3 497 .. 505 982 .. 503 985 .. 0.96 Alignments for each domain: == domain 1 score: 610.5 bits; conditional E-value: 1.1e-187 TIGR01238 3 ygegrknslGvdlaneselksleeqllkaaakkfqaapivgekakaegeaqpvknpadrkdivGqvseadaae 75 + gr+ns G dla + l+ +e+ + f+a pi++e + a g+ v++pa vG+v a++ + NCBI__GCF_000015985.1:WP_011840638.1 505 FP-GRRNSRGFDLAAAEDLALIESARSPHRTALFDARPILAE-SLAGGQRAEVTDPATG-ARVGNVLPASLPD 574 54.5************************************55.567888888****975.68*********** PP TIGR01238 76 vqeavdsavaafaewsatdakeraailerladlleshmpelvallvreaGktlsnaiaevreavdflryyakq 148 v+ a++ av w+a + ++ra +l+r+adl e + all reaGktl +a+ e+reavdflryya + NCBI__GCF_000015985.1:WP_011840638.1 575 VETALHHAV----PWEARP-EDRAEVLRRAADLYEASFGPIFALLAREAGKTLPDAVGELREAVDFLRYYAAE 642 999988776....699975.79*************************************************** PP TIGR01238 149 vedvldeesakalGavvcispwnfplaiftGqiaaalaaGntviakpaeqtsliaaravellqeaGvpagviq 221 + +a +lG+vvcispwnfplaiftGqiaaal+aGn+v+akpaeqt+liaa avell+ aGvpa+++q NCBI__GCF_000015985.1:WP_011840638.1 643 G-EA---LTAPPLGTVVCISPWNFPLAIFTGQIAAALMAGNAVLAKPAEQTPLIAALAVELLHRAGVPATALQ 711 9.44...46779************************************************************* PP TIGR01238 222 llpGrGedvGaaltsderiaGviftGstevarlinkalakredapvpliaetGGqnamivdstalaeqvvadv 294 llpG G +vGaalt d+r++Gv+ftGste+ar i++++a+ d+ +pliaetGG namivdstal+eq v+d+ NCBI__GCF_000015985.1:WP_011840638.1 712 LLPGEGGTVGAALTRDPRVGGVVFTGSTETARAIRQSMADHLDPAAPLIAETGGLNAMIVDSTALPEQAVRDI 784 ************************************************************************* PP TIGR01238 295 lasafdsaGqrcsalrvlcvqedvadrvltlikGamdelkvgkpirlttdvGpvidaeakqnllahiekmkak 367 lasaf+saGqrcsalr l++qedva+ vl+++ Gamdel+ g+p l td+Gpvidaea+ + i + +a NCBI__GCF_000015985.1:WP_011840638.1 785 LASAFQSAGQRCSALRCLYLQEDVAESVLEMLCGAMDELQAGEPWDLATDLGPVIDAEAQAGIRDWIAAARAE 857 ************************************************************************* PP TIGR01238 368 akkvaqvkleddvesekgtfvaptlfelddldelkkevfGpvlhvvrykadeldkvvdkinakGygltlGvhs 440 ++ ++q+ + +gtfv pt++ + ++ e+++e+fGpvlhv ++a e+d+v+ +i+a+Gyglt+G+hs NCBI__GCF_000015985.1:WP_011840638.1 858 GRVLKQMAVPA-----RGTFVGPTVIRVGSIREMDREIFGPVLHVATFRATEIDRVIAEIDATGYGLTFGLHS 925 *******9998.....9******************************************************** PP TIGR01238 441 rieetvrqiekrakvGnvyvnrnlvGavvGvqpfGGeGlsGtGpkaGGplylyrltr 497 ri++ v+ + +r +vGn+yvnrn++GavvG qpfGGeGlsGtGpkaGGp yl rl++ NCBI__GCF_000015985.1:WP_011840638.1 926 RIDDRVQALVGRLRVGNIYVNRNQIGAVVGSQPFGGEGLSGTGPKAGGPAYLARLVS 982 ******************************************************987 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (500 nodes) Target sequences: 1 (1146 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 30.81 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory