Alignments for a candidate for etoh-dh-nad in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for etoh-dh-nad in Cereibacter sphaeroides ATCC 17029

Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate WP_011840648.1 RSPH17029_RS04355 choline dehydrogenase

Query= BRENDA::Q76HN6
         (526 letters)



>NCBI__GCF_000015985.1:WP_011840648.1
          Length = 548

 Score =  377 bits (967), Expect = e-109
 Identities = 223/528 (42%), Positives = 306/528 (57%), Gaps = 12/528 (2%)

Query: 3   FDYLIVGAGSAGCVLANRLSADPSVTVCLLEAGPEDRSPLIHTPLGLAAILPTRHVNWAF 62
           FDY+IVGAGSAGC +A RL  +   +V ++E G  D  P I  P  L+  +     +W  
Sbjct: 2   FDYIIVGAGSAGCAMAYRLG-EAGRSVLVVEHGGTDAGPFIQMPAALSYPMNMGIYDWGL 60

Query: 63  KTTPQPGLGGRVGYQPRGKVLGGSSSINGMIYIRGHQDDFNDWQALGNEGWGFDDVLPYF 122
           KT P+P L GRV   PRGKV+GGSSSINGM+Y+RGH  DF+ W   G  GWGF DVLPYF
Sbjct: 61  KTEPEPHLDGRVLATPRGKVIGGSSSINGMVYVRGHARDFDHWAESGATGWGFADVLPYF 120

Query: 123 RKSE-MHHGGSSEYHGGDGELYVSPANR-HAASEAFVESALRAGHSYNPDFNGATQEGAG 180
           ++ E  H  G  E+ G DG L+V+   R +    AF+E+  +AG+    D+NGA QEG G
Sbjct: 121 KRMENWHVPGDVEWRGHDGPLHVTRGPRSNPLFNAFIEAGRQAGYPVTDDYNGAAQEGFG 180

Query: 181 YYDVTIRDGRRWSTATAFLKPVRHRSNLTVLTHTHVESIVLLGKQATGVQALIKGSRVHL 240
             + TI  GRRWS A A+L+P   R  +  LT      +V+   +A GV+   +G R  +
Sbjct: 181 PMEATIWQGRRWSAANAYLRPAMKRFGVQ-LTRALALKVVIEEGRAVGVEVQRRGGREVI 239

Query: 241 RARKEVILSAGAFGSPHLLMLSGIGSAAELEPQGIAPRHELPGVGQNLQDHADVVLCYKS 300
           RA +EVIL+A +  +P LLMLSGIGSAA L   GI    + PGVG+NLQDH +V + + S
Sbjct: 240 RAGREVILAASSLNTPKLLMLSGIGSAAHLAEHGIPVVADRPGVGRNLQDHLEVYMQFAS 299

Query: 301 ND--TSLLGFSLSGGVKMGKAMFDYARHRNGPVASNCAEAGAFLKTDPGLERPDIQLHSV 358
               T    ++L G V +G       R   G  ASN  EA AF+++ PG++ PDIQ H +
Sbjct: 300 LQPVTLFKHWNLRGKVSIGAQWLFTGR---GLGASNQFEACAFIRSKPGVDYPDIQYHFL 356

Query: 359 IGTVDDHNRKLHWGHGFSCHVCVLRPKSIGSVGLASPDPRKAPRIDPNFLAHDDDVATLL 418
              V    +    GHGF  HV  +R  S GSV L S DP   P I  N+++ ++D     
Sbjct: 357 PIAVRYDGKAAAEGHGFQVHVGPMRSPSRGSVTLRSADPEAPPVIRFNYMSTEEDWQDFR 416

Query: 419 KGYRITRDIIAQTPMASFGLRDMY-SAGLHNDEQLIELLRKRTDTIYHPIGTCKMGQ--D 475
           +  R+TR+I  Q     F   ++   A   +D ++   +R   ++ YHP GT ++G+  D
Sbjct: 417 RCVRLTREIFGQEAFKPFVRHEIQPGAACASDAEIDAFIRAHVESAYHPCGTARIGRADD 476

Query: 476 EMAVVDSQLRVHGIEGLRVVDASIMPTLVGGNTNAAAIMIAERAAEWI 523
            MAVVD + RV G+EGLRV D+SI P +  GN NA +IM+ E+A++ I
Sbjct: 477 PMAVVDPECRVIGVEGLRVADSSIFPRVTNGNLNAPSIMVGEKASDHI 524


Lambda     K      H
   0.319    0.137    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 890
Number of extensions: 47
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 526
Length of database: 548
Length adjustment: 35
Effective length of query: 491
Effective length of database: 513
Effective search space:   251883
Effective search space used:   251883
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources