Align Methylcrotonoyl-CoA carboxylase subunit alpha, mitochondrial; MCCase subunit alpha; 3-methylcrotonyl-CoA carboxylase 1; 3-methylcrotonyl-CoA carboxylase biotin-containing subunit; 3-methylcrotonyl-CoA:carbon dioxide ligase subunit alpha; EC 6.4.1.4 (characterized)
to candidate WP_011840651.1 RSPH17029_RS04405 acetyl/propionyl/methylcrotonyl-CoA carboxylase subunit alpha
Query= SwissProt::Q99MR8 (717 letters) >NCBI__GCF_000015985.1:WP_011840651.1 Length = 666 Score = 533 bits (1372), Expect = e-155 Identities = 297/686 (43%), Positives = 407/686 (59%), Gaps = 44/686 (6%) Query: 47 KVLIANRGEIACRVIRTAKKMGVQSVAVYSEADRNSMHVDMADEAYSIGPAPSQQSYLAM 106 K+LIANRGEIACRVI+TA+KMG+Q+VAVYS+ADRN++HV MADEA IGP P+ QSY+ + Sbjct: 4 KILIANRGEIACRVIKTARKMGIQTVAVYSDADRNALHVSMADEAIHIGPPPANQSYIVI 63 Query: 107 EKIIQVAKSSAAQAIHPGYGFLSENMEFAELCKQEGIIFIGPPSSAIRDMGIKSTSKSIM 166 +KI++ K+S A+A+HPGYGFLSE M+FA + G++FIGPPS AI MG K TSK + Sbjct: 64 DKIMEAIKASGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPSGAIEAMGDKITSKKLA 123 Query: 167 AAAGVPVVEGYHGKDQSDQCLREHAGKIGYPVMIKAVRGGGGKGMRIVRSEREFQEQLES 226 AGV V GY G + + +IGYPVMIKA GGGGKGMRI SE E +E ES Sbjct: 124 KEAGVSTVPGYMGLIADADEAVKISNEIGYPVMIKASAGGGGKGMRIAWSEAEVKEGFES 183 Query: 227 ARREAKKSFNDDAMLIEKFVDTPRHVEVQVFGDHHGNAVYLFERDCSVQRRHQKIIEEAP 286 ++ EA SF DD + IEKFV PRH+E+QV D+HGN VYL ER+CS+QRR+QK+IEEAP Sbjct: 184 SKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADNHGNCVYLHERECSIQRRNQKVIEEAP 243 Query: 287 APGINPEVRRKLGEAAVRAAKAVKYVGAGTVEFIMDSRHNFYFMEMNTRLQVEHPVTEMI 346 +P ++ R+ +GE A AKAV Y AGTVEFI+D + NFYF+EMNTRLQVEHPVTE+I Sbjct: 244 SPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQKNFYFLEMNTRLQVEHPVTELI 303 Query: 347 TGTDLVEWQLRIAAGEKIPLSQEEIPLQGHAFEARIYAEDPDNNFMPGAGPLVHLSTPSA 406 TG DLVE +R+AAGEK+P Q ++ + G A E+R+YAEDP NF+P G L P Sbjct: 304 TGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRLTRYRPPVE 363 Query: 407 DMS----TRIETGVRQGDEVSVHYDPMIAKLVVWASDRQSALSKLRYCLHQYNIVGLRSN 462 ++ R +TGV +G E+S++YDPMIAKL WA R++A+ ++R L + + G+ N Sbjct: 364 SVTPTSVVRNDTGVYEGGEISMYYDPMIAKLCTWAPTREAAIEEMRLALDTFEVEGIGHN 423 Query: 463 VDFLLRLSGHPEFEAGNVHTDFIPQHHKD----LLPSHSTIAKESVCQAALGLILKEKEM 518 + F+ + HP F G++ T FI + + D + T+ + + AA+ + Sbjct: 424 LPFVGAVMDHPRFVKGDITTAFIAEEYPDGFQGAVLDEPTLRRVAAAAAAMNRV------ 477 Query: 519 TSAFKLHTQDQFSPFSFSSGRRLNISYTRNMTLRSGKSDIVIA---------VTYNRDGS 569 + RR IS T N R D V+A + +R+GS Sbjct: 478 -----------------AEIRRTRISGTMNNHERHVGVDWVVALQGESYHVSIAADREGS 520 Query: 570 YDMQIDNKSFRVLGDLSSEDGCTYLKSSINGVARKSKFILLDNTVHLFSMEGSIEVGIPV 629 D S RV D + L + K I + + L + + V P Sbjct: 521 TVSFSDGSSLRVTSDWTPGQPLASLMVDGKPLVMKVGKIPMGFRLRLRGADLKVNVRTPR 580 Query: 630 PKYLSPVSAEGAQGGT----IAPMTGTIEKVFVKAGDRVKAGDSLMVMIAMKMEHTIKAP 685 L+ + E T + PM G + K+ V GD V+ G +L + AMKME+ ++A Sbjct: 581 QAELALLMPEKLPPDTSKYLLCPMPGLVVKINVAEGDEVQEGQALATVEAMKMENILRAE 640 Query: 686 KDGRIKKVFFSEGAQANRHAPLVEFE 711 + G +KK+ + GA ++EFE Sbjct: 641 RRGTVKKIAAAPGASLRVDDVIMEFE 666 Lambda K H 0.318 0.133 0.382 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1087 Number of extensions: 39 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 717 Length of database: 666 Length adjustment: 39 Effective length of query: 678 Effective length of database: 627 Effective search space: 425106 Effective search space used: 425106 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory