Alignments for a candidate for ech in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Cereibacter sphaeroides ATCC 17029

Align 3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35); short-chain-enoyl-CoA hydratase (EC 4.2.1.150) (characterized)
to candidate WP_011840656.1 RSPH17029_RS04435 enoyl-CoA hydratase/isomerase family protein

Query= BRENDA::A4YDS4
         (651 letters)



>NCBI__GCF_000015985.1:WP_011840656.1
          Length = 733

 Score =  189 bits (481), Expect = 3e-52
 Identities = 129/396 (32%), Positives = 200/396 (50%), Gaps = 24/396 (6%)

Query: 2   KVTVIGSGVMGHGIAELAAIAGNEVWMNDISTEILQQAMERIKWSLSKLRESGSLK-EGV 60
           K+ VIG+G+MG GIA +AA AG EV + D + E   +     +  L K  + G +  E  
Sbjct: 329 KLGVIGAGMMGAGIAHVAANAGIEVVLIDATQEAADRGKAHSEGLLDKGMKRGKVSAEKK 388

Query: 61  EQVLARIHPETDQAQALKGSDFVIEAVKEDLELKRTIFRNAEAHASPSAVLATNTSSLPI 120
            +VL RI   TD A AL G D ++EAV ED  +K  + +  EA   P  + ATNTS+LPI
Sbjct: 389 AEVLGRIAATTDYA-ALSGCDLIVEAVFEDPAVKAEVTQKVEAAVGPDCIFATNTSTLPI 447

Query: 121 SEIASVLKSPQRVVGMHFFNPPVLMPLVEIVRGKDTSDEVVKTTAEMAKSMNKETIVVKD 180
           S +A   + P + +G+HFF+P   M LVEI+RGK T D  V    +  + + K  IVV D
Sbjct: 448 SGLAKASRDPAQFIGIHFFSPVDKMMLVEIIRGKATGDRAVAKALDFVRQIRKTPIVVHD 507

Query: 181 VPGFFVNRVLLRIMEAGCYLVEKGIASIQEVDSSAIEELGFPMGVFLLADYTGLDIGYSV 240
              F+ NR ++  +  G  +V +G+A    +  +A + +G P+G   L D T +D+G  +
Sbjct: 508 ARFFYANRCIIPYLNEGIRMVAEGVA--PALIENAAKLVGMPLGPLQLVDETSIDLGVKI 565

Query: 241 WKAVTARGFKAFPCSSTE----KLVSQGKLGVKSGSGYYQYPSPGKF------------V 284
            KA  A   +A+P ++ +     L  +G+LG K+G+G+Y Y + GK             +
Sbjct: 566 AKATKAALGEAYPDAAVDAVIFPLADEGRLGRKAGAGFYAYDAAGKRDGLWPGLADRWPL 625

Query: 285 RPTLPSTSKKLGRYLISPAVNEVSYLLREGIVGKDDAEKGCVLGLGLPK---GILSYADE 341
             T P  ++   R L +  +  V  L    +    + + G +LG G      G  S+ D 
Sbjct: 626 AETQPELAEVQHRLLFAQVLEAVRALEEGVLTDIREGDVGAILGWGFAPWSGGPFSWLDM 685

Query: 342 IGIDVVVNTLEEMRQTSGMDHYSPDPLLLSMVKEGK 377
           IG    V   E + +  G   ++P  LL+ M  +G+
Sbjct: 686 IGAPRAVEICEGLAERHG-TRFAPPKLLVEMAGKGE 720



 Score = 64.3 bits (155), Expect = 2e-14
 Identities = 49/177 (27%), Positives = 88/177 (49%), Gaps = 19/177 (10%)

Query: 428 EINQALDSLEEREDVRVIAITGQGRVFSAGADVT------EFGSLTPVKAMIAS-RKFHE 480
           E++  + +  +   V+ + +T   + F+AG D+       E G   P +A+     + H 
Sbjct: 35  ELDGLIAAALDDPSVKGVILTSGKKDFAAGMDLNVIARMKEEGGDDPARAVFEGVMEMHA 94

Query: 481 VFMKIQFLT---------KPVIAVINGLALGGGMELALSADFRVASKT--AEMGQPEINL 529
           V  +I+            KP++A + G ALG G+EL L+    +A+    A++G PEI +
Sbjct: 95  VLRRIERAGMDPKTLKGGKPIVAALPGTALGLGLELPLACHRIIAADNPKAKIGLPEIMV 154

Query: 530 GLIPGGGGTQRL-SRLSGRKGLELVLTGRRVKAEEAYRLGIVEFLAEPEELESEVRK 585
           G+ PGGGGT RL  RL       L+L G+    + A  +G+V+ +   ++L +  ++
Sbjct: 155 GIFPGGGGTTRLVRRLGAMMAAPLLLEGKLNDPKGAKAMGVVDEVVPADQLLARAKE 211



 Score = 42.4 bits (98), Expect = 8e-08
 Identities = 29/94 (30%), Positives = 48/94 (51%), Gaps = 8/94 (8%)

Query: 300 ISPAVNEVSYLLREGIVGK--DDAEKGCVLGLGLPKGILSYADEIGIDVVVNTLEEMRQT 357
           I P +NE   ++ EG+     ++A K     +G+P G L   DE  ID+ V   +  +  
Sbjct: 517 IIPYLNEGIRMVAEGVAPALIENAAKL----VGMPLGPLQLVDETSIDLGVKIAKATKAA 572

Query: 358 SGMDH--YSPDPLLLSMVKEGKLGRKSGQGFHTY 389
            G  +   + D ++  +  EG+LGRK+G GF+ Y
Sbjct: 573 LGEAYPDAAVDAVIFPLADEGRLGRKAGAGFYAY 606


Lambda     K      H
   0.316    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1016
Number of extensions: 63
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 3
Length of query: 651
Length of database: 733
Length adjustment: 39
Effective length of query: 612
Effective length of database: 694
Effective search space:   424728
Effective search space used:   424728
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources