Alignments for a candidate for liuB in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for liuB in Cereibacter sphaeroides ATCC 17029

Align Methylcrotonoyl-CoA carboxylase (EC 6.4.1.4) (characterized)
to candidate WP_011840850.1 RSPH17029_RS05950 biotin/lipoyl-binding protein

Query= reanno::Smeli:SM_b21124
         (662 letters)



>NCBI__GCF_000015985.1:WP_011840850.1
          Length = 646

 Score =  672 bits (1735), Expect = 0.0
 Identities = 370/658 (56%), Positives = 453/658 (68%), Gaps = 15/658 (2%)

Query: 1   MFSKLLIANRGEIACRIIRTARRLGIRTVAVYSDADGDALHVALADEAIRIGGAPAAESY 60
           MF K+LIANRGEIA RI  TARRLG+ TVAV+SDAD +ALHVA+ADEA+ IGG   A+SY
Sbjct: 1   MFRKILIANRGEIAVRIAATARRLGVATVAVHSDADAEALHVAVADEAVPIGGPQPADSY 60

Query: 61  LASAPIVQAARSVGAQAIHPGYGFLSENADFAEAVAEAGMIFVGPPPAAIRAMGLKDAAK 120
           L    +++AA + GA+AIHPGYGFLSEN +F EAV  AG++F+GP  AAIRAMGLKDAAK
Sbjct: 61  LRGERLIEAALATGAEAIHPGYGFLSENPEFVEAVEAAGLVFIGPSAAAIRAMGLKDAAK 120

Query: 121 ALMERSGVPVVPGYHGEEQDASFLADRAREIGYPVLIKARAGGGGKGMRRVERQEDFGPA 180
           ALM  +GVPVVPGYHG +Q+   LA  A++IGYPVLIKA AGGGGKGMRRVER E+F  A
Sbjct: 121 ALMAEAGVPVVPGYHGADQEPEHLALMAQDIGYPVLIKAVAGGGGKGMRRVERAEEFAAA 180

Query: 181 LEAARREAESAFGDGSVLLERYLTKPRHIEMQVFGDRHGNIVHLFERDCSLQRRHQKVIE 240
           L +AR EA +AFG+ +VL+E+++  PRHIE+QVFGD   + VHL+ERDCSLQRRHQKVIE
Sbjct: 181 LASARAEARTAFGNEAVLIEKWIDCPRHIELQVFGD-GTDAVHLYERDCSLQRRHQKVIE 239

Query: 241 EAPAPGMTAEVRRAMGDAAVRAAQAIGYVGAGTVEFIADVTNGLWPDHFYFMEMNTRLQV 300
           EAPAPGMT  +R AMG+AAVRAA+AIGY GAGTVEFI D + GL PD F+FMEMNTRLQV
Sbjct: 240 EAPAPGMTPGMRAAMGEAAVRAARAIGYRGAGTVEFIVDGSAGLSPDRFWFMEMNTRLQV 299

Query: 301 EHPVTEAITGIDLVEWQLRVASGEPLPKKQADISMNGWAFEARLYAEDPARGFLPATGRL 360
           EHPVTEA+TGIDLVEWQLRVA+GEPLP  Q DI + G AFEARLYAED   GFLPATGRL
Sbjct: 300 EHPVTEAVTGIDLVEWQLRVAAGEPLPLAQKDIPLRGHAFEARLYAEDVPAGFLPATGRL 359

Query: 361 TELSFPEGTSRVDSGVRQGDTITPYYDPLIAKLIVHGQNRSAALGRLQDALKECRIGGTV 420
             L F +G  R D+GVR GD I+P+YDP+IAK++ HG +R++AL RL  AL+E  + GTV
Sbjct: 360 DHLRFADGI-RADTGVRTGDRISPWYDPMIAKIVAHGPSRASALNRLARALEETEVAGTV 418

Query: 421 TNRDFLIRLTEEHDFRSGHPDTGLIDREIERLTAPVAPGDEALALAAIFSTGALDPNRST 480
           TN  FL RL +   F +G  DTGLI R++  L AP     E +A A + +     P    
Sbjct: 419 TNLAFLCRLAQHPGFAAGEVDTGLIGRDLAPLIAPATLAPETVAAALLVAELGETP---- 474

Query: 481 DPWSSLGSWQIWGDAHRMVVIEHADVRATVTLASRGRDQFAVRAGASTLPVLVLDRFEGG 540
              + L  + +W    R V + H   +    L      +  V  G + L   +  R EG 
Sbjct: 475 ---APLQGFSLWAPLRRHVRLRHGSEKIAAALTVEAPGRATVELGEARLAARL--RPEGW 529

Query: 541 ARLEVAGQKRLIRFSRDREALTLFHGGRNLVFHVPDGLTGGQSSEIADDELVAPMPGLVK 600
           A +E     R++R       + +F     + F +PD L  G          ++PMPGLV+
Sbjct: 530 A-VEGRPPARVLRIGAQ---VHVFARTGTVSFEIPDPLDRGTEEAGQGGLTLSPMPGLVR 585

Query: 601 LVRVGAGDAVTKGQALVVMEAMKMELTLSASREGTIANVHVAEGAQVSEGTVLVTLME 658
            V V AGD V KG  L V+EAMKME  L+A+R+GT+A V V  GAQV  G  LV L E
Sbjct: 586 EVSVRAGDRVEKGDRLAVIEAMKMEHVLTAARDGTVAEVLVEAGAQVEAGAALVRLEE 643


Lambda     K      H
   0.319    0.135    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1250
Number of extensions: 48
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 662
Length of database: 646
Length adjustment: 38
Effective length of query: 624
Effective length of database: 608
Effective search space:   379392
Effective search space used:   379392
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources