Alignments for a candidate for pccA in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for pccA in Cereibacter sphaeroides ATCC 17029

Align propionyl-CoA carboxylase α subunit (EC 6.4.1.3) (characterized)
to candidate WP_011840850.1 RSPH17029_RS05950 biotin/lipoyl-binding protein

Query= metacyc::MONOMER-13589
         (666 letters)



>NCBI__GCF_000015985.1:WP_011840850.1
          Length = 646

 Score =  488 bits (1256), Expect = e-142
 Identities = 291/676 (43%), Positives = 391/676 (57%), Gaps = 44/676 (6%)

Query: 1   MFKKILIANRGEIACRVIKTARKMGIQTVAVYSDADRNALHVSMADEAIHIGPPPANQSY 60
           MF+KILIANRGEIA R+  TAR++G+ TVAV+SDAD  ALHV++ADEA+ IG P    SY
Sbjct: 1   MFRKILIANRGEIAVRIAATARRLGVATVAVHSDADAEALHVAVADEAVPIGGPQPADSY 60

Query: 61  IVIDKIMEAIKASGAEAVHPGYGFLSERMDFAAALEAAGVVFIGPPSGAIEAMGDKITSK 120
           +  ++++EA  A+GAEA+HPGYGFLSE  +F  A+EAAG+VFIGP + AI AMG K  +K
Sbjct: 61  LRGERLIEAALATGAEAIHPGYGFLSENPEFVEAVEAAGLVFIGPSAAAIRAMGLKDAAK 120

Query: 121 KLAKEAGVSTVPGYMGLIADADEAVKISNEIGYPVMIKASAGGGGKGMRIAWSEAEVKEG 180
            L  EAGV  VPGY G   + +    ++ +IGYPV+IKA AGGGGKGMR      E    
Sbjct: 121 ALMAEAGVPVVPGYHGADQEPEHLALMAQDIGYPVLIKAVAGGGGKGMRRVERAEEFAAA 180

Query: 181 FESSKNEAANSFGDDRIFIEKFVTQPRHIEIQVLADKHGNCVYLHERECSIQRRNQKVIE 240
             S++ EA  +FG++ + IEK++  PRHIE+QV  D   + V+L+ER+CS+QRR+QKVIE
Sbjct: 181 LASARAEARTAFGNEAVLIEKWIDCPRHIELQVFGD-GTDAVHLYERDCSLQRRHQKVIE 239

Query: 241 EAPSPFLDEATRKAMGEQACALAKAVGYASAGTVEFIVDGQ-----KNFYFLEMNTRLQV 295
           EAP+P +    R AMGE A   A+A+GY  AGTVEFIVDG        F+F+EMNTRLQV
Sbjct: 240 EAPAPGMTPGMRAAMGEAAVRAARAIGYRGAGTVEFIVDGSAGLSPDRFWFMEMNTRLQV 299

Query: 296 EHPVTELITGIDLVEQMIRVAAGEKLPFQQSDLKINGWAMESRLYAEDPYRNFLPSIGRL 355
           EHPVTE +TGIDLVE  +RVAAGE LP  Q D+ + G A E+RLYAED    FLP+ GRL
Sbjct: 300 EHPVTEAVTGIDLVEWQLRVAAGEPLPLAQKDIPLRGHAFEARLYAEDVPAGFLPATGRL 359

Query: 356 TRYRPPVESVTPTSVVRNDTGVYEGGEISMYYDPMIAKLCTWAPTREAAIEEMRLALDTF 415
              R           +R DTGV  G  IS +YDPMIAK+    P+R +A+  +  AL+  
Sbjct: 360 DHLR-------FADGIRADTGVRTGDRISPWYDPMIAKIVAHGPSRASALNRLARALEET 412

Query: 416 EVEGIGHNLPFVGAVMDHPRFVKGDITTAFIAEEYPDGFQGAVLDEPTLRRVAAAAAAMN 475
           EV G   NL F+  +  HP F  G++ T  I  +       A L   T+      A    
Sbjct: 413 EVAGTVTNLAFLCRLAQHPGFAAGEVDTGLIGRDLAPLIAPATLAPETVAAALLVAELGE 472

Query: 476 RVAEIRRTRISGTMNNHERHVGVDWVVALQGESYHVSIAADREGSTVSFSDGSSLRVTSD 535
             A ++   +   +  H         V L+  S  ++ A   E    +  +    R+ + 
Sbjct: 473 TPAPLQGFSLWAPLRRH---------VRLRHGSEKIAAALTVEAPGRATVELGEARLAAR 523

Query: 536 WTPGQPLASLMVDGRPLVMKVGKIPMGFRLRLRGADLKVNVRTPRQAELALLMPEKLPPD 595
             P        V+GRP            R+   GA + V  RT     ++  +P+ L   
Sbjct: 524 LRP----EGWAVEGRPPA----------RVLRIGAQVHVFART---GTVSFEIPDPLDRG 566

Query: 596 TSK-----YLLCPMPGLVVKINVAEGDEVQEGQALATVEAMKMENILRAERRGTVKKIAA 650
           T +       L PMPGLV +++V  GD V++G  LA +EAMKME++L A R GTV ++  
Sbjct: 567 TEEAGQGGLTLSPMPGLVREVSVRAGDRVEKGDRLAVIEAMKMEHVLTAARDGTVAEVLV 626

Query: 651 APGASLRVDDVIMEFE 666
             GA +     ++  E
Sbjct: 627 EAGAQVEAGAALVRLE 642


Lambda     K      H
   0.318    0.134    0.386 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1164
Number of extensions: 53
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 666
Length of database: 646
Length adjustment: 38
Effective length of query: 628
Effective length of database: 608
Effective search space:   381824
Effective search space used:   381824
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources