Align GlpV, component of Glycerol uptake porter, GlpSTPQV (characterized)
to candidate WP_011840943.1 RSPH17029_RS06695 ABC transporter substrate-binding protein
Query= TCDB::G3LHZ3 (569 letters) >NCBI__GCF_000015985.1:WP_011840943.1 Length = 574 Score = 923 bits (2386), Expect = 0.0 Identities = 441/571 (77%), Positives = 496/571 (86%), Gaps = 9/571 (1%) Query: 2 TTTAGLLLAMTASAYAGMDEAKTFLDKEVGDLSTLPRADQEKEMQWFVDAAKPFAGMDIK 61 T A L+A+ A A+A M+ AK FLD E+GDLS+L RA+QE EMQWF+DAA+PFAGM+IK Sbjct: 8 TAMALCLMAVAAPAWADMEAAKKFLDAEIGDLSSLSRAEQEAEMQWFIDAAQPFAGMEIK 67 Query: 62 VVSETITTHEYESKVLAPAFTAITGIKITHDLIGEGDVVEKLQTQMQSGENIYDAYINDS 121 VVSETITTHEYESKVLAPAF+AITGIKI+HDLIGEGDVVEKLQTQMQSGENIYDAYINDS Sbjct: 68 VVSETITTHEYESKVLAPAFSAITGIKISHDLIGEGDVVEKLQTQMQSGENIYDAYINDS 127 Query: 122 DLIGTHWRYQQARSLTDWMANEGKDVTNPGLDIDDFIGKSFTTAPDGKLYQLPDQQFANL 181 DLIGTHWRY+QARSLTDWMAN GKDVTNPGLD+ D+IG FTTAPDG+LYQLPDQQFANL Sbjct: 128 DLIGTHWRYKQARSLTDWMANAGKDVTNPGLDLQDYIGLKFTTAPDGELYQLPDQQFANL 187 Query: 182 YWFRYDWFNDEKNKADFKAKYGYDLGVPVNWSAYEDIAEFFTGREIDG----KKVFGHMD 237 YWFR DWF+D KA FK KYGY+LGVP+NWSAYEDIAEFFTGR++ K FG MD Sbjct: 188 YWFRADWFDDADTKAAFKEKYGYELGVPLNWSAYEDIAEFFTGRDMSALGGPKSAFGSMD 247 Query: 238 YGKKDPSLGWRFTDAWLSMAGNGDKGIPNGKPVDEWGIKVDENSRPVGSCVARGGDTNGP 297 YGKKDPSLGWR+TDAW+SMAG GDKG PNG PVDEWGI+VDENSRPVGSCVARGG TN Sbjct: 248 YGKKDPSLGWRYTDAWMSMAGMGDKGDPNGLPVDEWGIRVDENSRPVGSCVARGGATNDA 307 Query: 298 AAVYSIQKYLDWMKAYAPAAAQGMTFSESGPVPSQGEVAQQMFTYTAFTADFVKEGLPVV 357 AAVY+I K ++W++ YAP A GMTFSESGPVP+QGEVAQQ+F YTAFTAD VKEGLPV+ Sbjct: 308 AAVYAITKSIEWLQKYAPPQAAGMTFSESGPVPAQGEVAQQIFWYTAFTADMVKEGLPVM 367 Query: 358 NADGTPKWRFAPSPHGVYWKDGMKLGYQDAGSWTLMKSTPDDRAKAAWLYAQFVTSKTVD 417 N DGTPKWR APSPHG YW +G K+GYQD GSWTL+KSTPD+RAKAAWLYAQFV+SKTVD Sbjct: 368 NEDGTPKWRMAPSPHGAYWSEGTKVGYQDVGSWTLLKSTPDERAKAAWLYAQFVSSKTVD 427 Query: 418 VKKSHMGLTFIRQSTLDHKSFTDRAPKLGGLIEFYRSPARLQWSPTGTNVPDYPKLAQLW 477 VKKSH+GLTF+R+ST+ H+SFTDRAPKLGGL+EFYRSPAR+QWSPTGTNVPDYPKLAQLW Sbjct: 428 VKKSHVGLTFVRESTIQHQSFTDRAPKLGGLVEFYRSPARVQWSPTGTNVPDYPKLAQLW 487 Query: 478 WQAIGDASSGAKTAQEAMDSLCSEQEKVLQRLERAKVQGDIGPKLAEEHDLAYWNADAVK 537 WQ IGDA SGAK+ QEA+D+LC+EQEKV+ RLERA VQGD+GPKL EE D W DA Sbjct: 488 WQNIGDAMSGAKSPQEALDALCAEQEKVMARLERAGVQGDLGPKLNEEKDPQEW-LDAPG 546 Query: 538 NGNLAPQLKIENEKDKPVTVNYDELVKSWQK 568 AP K+ENEK + T++YDEL+KSWQK Sbjct: 547 ----APVAKLENEKPQGETISYDELIKSWQK 573 Lambda K H 0.315 0.132 0.409 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1372 Number of extensions: 47 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 569 Length of database: 574 Length adjustment: 36 Effective length of query: 533 Effective length of database: 538 Effective search space: 286754 Effective search space used: 286754 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory