Alignments for a candidate for tctA in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for tctA in Cereibacter sphaeroides ATCC 17029

Align Tricarboxylic transport TctA (characterized, see rationale)
to candidate WP_011842050.1 RSPH17029_RS15390 tripartite tricarboxylate transporter permease

Query= uniprot:E4PJQ9
         (508 letters)



>NCBI__GCF_000015985.1:WP_011842050.1
          Length = 498

 Score =  313 bits (801), Expect = 1e-89
 Identities = 188/506 (37%), Positives = 270/506 (53%), Gaps = 14/506 (2%)

Query: 1   METLGFLMDGFAVALTPYNLMFALFGAFVGTLIGCLPGLGPANGVAILIPLAFTLGLPPE 60
           METL +L+  F     P  L     G   G +IG LPGL    GVA+LIPL F  G+ P 
Sbjct: 1   METLSYLLPLFE----PATLGAIALGTLGGLIIGALPGLTATMGVALLIPLTF--GMSPV 54

Query: 61  TAMILLTAVYAGAMYGGRISSILLNIPGDEPAMMTCLDGYPMAQKGRAADALAVSAIASF 120
             + +L  +Y G +YGG +S+ILL  PG   +  T LDGYPMA +G A  AL ++ IAS 
Sbjct: 55  MGLNMLIGIYIGGIYGGCVSAILLRTPGTPSSAATVLDGYPMALRGEAGKALGLATIAST 114

Query: 121 AGGLIGTIGLIMLAPVLAKFALTFGPAEYFALFLLAFATLGGITGKNPVKTVVAATLGIM 180
            GGL   + L  LAP LA  AL FG AEYFAL       +  ++G + +K V+A  LGI+
Sbjct: 115 LGGLFAAVVLATLAPQLAGIALKFGAAEYFALAFFGLTIIASLSG-DILKGVIAGMLGIL 173

Query: 181 ISTVGIDISTGTQRYTFGVLELYEGIDFILAIVGLFAISELLFFVESR-MGRGRDKMNVG 239
           IS VG D  TG  RYTFGV     G  F  A++GLFA+SE+   +E    G G     VG
Sbjct: 174 ISCVGADPITGMLRYTFGVSGFASGFAFTPALIGLFALSEVFTQLERLGAGDGAQVQKVG 233

Query: 240 KLTLTMKELVMTIPTQLRGGVLGFISGVLPGAGASLGSFISYTLEKQVVGKKGKFGEGDI 299
                  +L  +    +RG ++G + G++PG G+   S+I+Y   ++      KFG G  
Sbjct: 234 GRWPAWADLRESRGALVRGSIIGTLIGIVPGTGSGTASWIAYNEARRASKTPEKFGTGHA 293

Query: 300 RGVVAPEAGNNGASSGALVPMLTLGVPGSGTTAVLLAMLISLNITPGPLMFTQNADIVWG 359
            G+ A E+ NN   + AL+P+L LGVPG   TAVL+  L+   + PGP++F  N D+V G
Sbjct: 294 PGICATESANNAVCAAALIPLLALGVPGDVVTAVLMGGLMIQGLAPGPMLFQTNPDVVVG 353

Query: 360 VIAALLIGNVLLLVLNIPLVGFFVKLLSVPPMYLLPIVTMVAFVGIYSISHSTFDLYFMV 419
           +     +  + + +  + L+  F ++L +P   L   + +  F+G Y+I+ +  DLY MV
Sbjct: 354 IFGGTFLATIFMFLFGMALIPVFSRILMIPRHILAVAIIVFCFIGAYAINLNPVDLYTMV 413

Query: 420 AFGVAGYFLRKLEIPLVPIILGLLLGPEMEKNLGHALVLSDGEWSVLWASPLAMGLWIVA 479
            FGV G+ + K       + + L+LGP +E NL   L+ +  +     + P+ +      
Sbjct: 414 GFGVLGWGMHKFGFSQAALCIALILGPMLESNLRRGLLQAGDDLVTFLSGPITL-----L 468

Query: 480 GLGLILPYLVGPLLRRRMNAAMKESP 505
            LGL L  L+ P +  R  AA K SP
Sbjct: 469 FLGLSLLSLLWPWIAAR-RAAGKPSP 493


Lambda     K      H
   0.325    0.144    0.425 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 836
Number of extensions: 56
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 508
Length of database: 498
Length adjustment: 34
Effective length of query: 474
Effective length of database: 464
Effective search space:   219936
Effective search space used:   219936
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources