Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_011842152.1 RSPH17029_RS16015 enoyl-CoA hydratase/isomerase family protein
Query= reanno::BFirm:BPHYT_RS13545 (706 letters) >NCBI__GCF_000015985.1:WP_011842152.1 Length = 655 Score = 499 bits (1285), Expect = e-145 Identities = 292/653 (44%), Positives = 391/653 (59%), Gaps = 12/653 (1%) Query: 8 DVVSRELRGKVLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAG 67 D++ R + L+T+ + PVNAL VR+ L A +AD++V AV++ GR F+ G Sbjct: 4 DLIRRSDFDGIALLTLANPPVNALGLAVRQRLTALTAELEADESVRAVVLTAEGRVFVGG 63 Query: 68 ADIREFGKPPVPPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLG 127 ADI EF +PP P LPDV IEA KP +AA++GAALGGG E+AL HYRI D A+LG Sbjct: 64 ADIAEFDRPPEAPHLPDVIAAIEASRKPWIAALNGAALGGGAELALGCHYRIFADTARLG 123 Query: 128 LPEVQLGLLPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRLGSSDDILAE 187 LPE LGL+PGAGGTQR PR IG A+++I +GR SA EA GL DR+ + + ++ E Sbjct: 124 LPETSLGLIPGAGGTQRLPRRIGLAPAIEVITAGRTLSAAEAREAGLADRIAAGE-LIPE 182 Query: 188 GLAYVHELLAAHAPVRRTRDAAALSDRAASLAAVATARAETAKKSRGLFSPLKIVDAVEA 247 LA+ L A + R AA L+D A ARA A+ +RG +P ++A+ A Sbjct: 183 ALAFARTLDGA---LPRPASAAPLADPGH--AFWDEARARIARAARGNPAPTAALEAIRA 237 Query: 248 AIEQPFDEGLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAAKPRTLNTIGVVG 307 + + F GLR ER+ FL S + A L H FFAER L+ R +P L GV+G Sbjct: 238 GVAEGFAAGLRAERETFLRLRASDEAAALRHLFFAERAALRPAALRGIEPVPLTRAGVIG 297 Query: 308 GGTMGAGIAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALMSR 367 GGTMG+GIA A+ AGL V + E +LA G +E +++ + +G M+R Sbjct: 298 GGTMGSGIAAALAAAGLEVRLTETGPEALAAGLERVEAIFEAQVKRGLTDRAGATERMAR 357 Query: 368 WSGSTSYDALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALASSV 427 +G+ ALA DLVIEAVFEDLAVK+ VF EL R+C A+LATNTSYLD + + + Sbjct: 358 VTGTVGLGALADCDLVIEAVFEDLAVKRRVFEELVRLCGPEAILATNTSYLDPERIVEGL 417 Query: 428 SRPADVIGLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGN 487 P I LHFFSPA +MKLLE+V + ++AT LA +L K PV+AG +GFIGN Sbjct: 418 PHPHRFIALHFFSPAQVMKLLEIVPLAATAPRILATGVALAARLGKIPVQAGNGEGFIGN 477 Query: 488 RVLAVYRSAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAATRN 547 R+L YR+ A+A++ GA+P +ID A+RAFG MGPF++ D+AG DI + AR+ A Sbjct: 478 RILKRYRAEAEALLLAGATPTEIDEAMRAFGLGMGPFEMQDMAGLDIAFRAREAARALGQ 537 Query: 548 PAARYVQIADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPRSF 607 DRL G G+KSG G+Y Y GSR P PE A+I A G P Sbjct: 538 DLPE--GPGDRLVRAGRLGRKSGGGWYDYAPGSRLPQPSPEAAALI-APLVTPGARP--- 591 Query: 608 TDEEIIRRYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYA 660 + EI R +AAM EG + E +A P D+D+ ++GYGFPR++GGPM +A Sbjct: 592 SGTEIADRLIAAMAEEGQRICDEGLAQSPSDIDLVEVHGYGFPRHKGGPMFHA 644 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1144 Number of extensions: 49 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 655 Length adjustment: 39 Effective length of query: 667 Effective length of database: 616 Effective search space: 410872 Effective search space used: 410872 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory