Align 3-oxoadipyl-CoA thiolase; EC 2.3.1.174 (characterized, see rationale)
to candidate WP_011842153.1 RSPH17029_RS16020 3-oxoadipyl-CoA thiolase
Query= uniprot:A0A2Z5MFE9 (400 letters) >NCBI__GCF_000015985.1:WP_011842153.1 Length = 399 Score = 539 bits (1389), Expect = e-158 Identities = 277/400 (69%), Positives = 321/400 (80%), Gaps = 1/400 (0%) Query: 1 MNDAYICDAIRTPIGRYGGALKDVRADDLGAVPIKALIQRNPGVDWRAVDDVIYGCANQA 60 M +ICD IRTPIGRYGGAL VRADDLGAVP+KAL+ RN GVDW AVDDVI+GCANQA Sbjct: 1 MTQVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQA 60 Query: 61 GEDNRNVARMSALLAGLPADAPGATINRLCGSGMDAVGTAARAIKAGEAQLMIAGGVESM 120 GEDNRNVARMSALLAGLP G TINRLCGSGMDAV TAAR I AGEA L+IAGGVESM Sbjct: 61 GEDNRNVARMSALLAGLPVGVTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESM 120 Query: 121 TRAPFVMGKAASAFTRQAEIHDTTIGWRFVNPLMKRQYGVDSMPETAENVAEQFGISRAD 180 +RAPFV+ KA +AFTR AEIHDTTIGWRFVNP M+ YGVD MP+T +NVA FGISR Sbjct: 121 SRAPFVLPKAGTAFTRSAEIHDTTIGWRFVNPAMEAAYGVDPMPQTGQNVACDFGISREA 180 Query: 181 QDAFALASQQKAARAQRDGTLAQEIVGVEIAQKKGDAIRVTLDEHPRETSLESLARLKGV 240 QDA ALASQ KAA AQ +G LAQEI V + Q++G+ +RV DEHPR T+ E+LA+LK + Sbjct: 181 QDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVEQDEHPRATTPEALAKLKPL 240 Query: 241 VRPDGTVTAGNASGVNDGACALLIASQQAAEQYGLRRRARVVGMATAGVEPRIMGIGPAP 300 G+VTAGN+SGVNDGA AL++AS+ A ++GL ARV+G ATAGV PRIMGIGP P Sbjct: 241 F-AGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPIARVLGGATAGVPPRIMGIGPVP 299 Query: 301 ATQKLLRQLGMTLDQLDVIELNEAFASQGLAVLRMLGLRDDDPRVNPNGGAIALGHPLGA 360 A KL+ +LG+T + DVIELNEAFA+QG+A LR LG+ ++D RVNPNGGAIALGHPLG Sbjct: 300 AALKLMARLGLTTEAFDVIELNEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGM 359 Query: 361 SGARLVTTALHQLERSNGRFALCTMCIGVGQGIALVIERL 400 SGAR+ TA QL+R+ GR AL MCIGVGQGIA+ +ER+ Sbjct: 360 SGARITGTAALQLQRTGGRRALSAMCIGVGQGIAIALERV 399 Lambda K H 0.319 0.134 0.386 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 515 Number of extensions: 11 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 400 Length of database: 399 Length adjustment: 31 Effective length of query: 369 Effective length of database: 368 Effective search space: 135792 Effective search space used: 135792 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 50 (23.9 bits)
Align candidate WP_011842153.1 RSPH17029_RS16020 (3-oxoadipyl-CoA thiolase)
to HMM TIGR02430 (pcaF: 3-oxoadipyl-CoA thiolase (EC 2.3.1.174))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02430.hmm # target sequence database: /tmp/gapView.679082.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02430 [M=400] Accession: TIGR02430 Description: pcaF: 3-oxoadipyl-CoA thiolase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.5e-202 657.6 13.2 3.9e-202 657.5 13.2 1.0 1 NCBI__GCF_000015985.1:WP_011842153.1 Domain annotation for each sequence (and alignments): >> NCBI__GCF_000015985.1:WP_011842153.1 # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 657.5 13.2 3.9e-202 3.9e-202 2 400 .] 3 399 .] 2 399 .] 0.99 Alignments for each domain: == domain 1 score: 657.5 bits; conditional E-value: 3.9e-202 TIGR02430 2 evyivdairtpiGrygGslssvraddlaavplkallarnpsldaaaiddvilGcanqaGednrnvarmaalla 74 +v+i+d irtpiGrygG+ls vraddl+avplkal+arn +d++a+ddvi+GcanqaGednrnvarm+alla NCBI__GCF_000015985.1:WP_011842153.1 3 QVFICDYIRTPIGRYGGALSPVRADDLGAVPLKALMARNGGVDWEAVDDVIFGCANQAGEDNRNVARMSALLA 75 79*********************************************************************** PP TIGR02430 75 GlpvsvpgttvnrlcgsgldalglaaraikaGeadlviaGGvesmsrapfvlGkadsafsrsakledttiGwr 147 Glpv v+gtt+nrlcgsg+da+ +aar i+aGeadlviaGGvesmsrapfvl ka +af+rsa+++dttiGwr NCBI__GCF_000015985.1:WP_011842153.1 76 GLPVGVTGTTINRLCGSGMDAVLTAARQIAAGEADLVIAGGVESMSRAPFVLPKAGTAFTRSAEIHDTTIGWR 148 ************************************************************************* PP TIGR02430 148 fvnpklkalyGvdsmpetaenvaeefgvsredqdafalrsqqrtaaaqakGffaeeivpveikqkkGeetvvd 220 fvnp+++a yGvd mp+t +nva +fg+sre qda+al sq ++aaaqa+G +a+ei+pv ++q++Ge++ v+ NCBI__GCF_000015985.1:WP_011842153.1 149 FVNPAMEAAYGVDPMPQTGQNVACDFGISREAQDAMALASQTKAAAAQANGRLAQEITPVLVPQRRGEPVRVE 221 ************************************************************************* PP TIGR02430 221 kdehlraettlealaklkavvredgtvtaGnasGvndGaaalllaseeavkrhgltprarilaaasaGveprv 293 +deh+r tt ealaklk+++ ++g+vtaGn+sGvndGaaal+lase av+rhgltp ar+l+ a+aGv pr+ NCBI__GCF_000015985.1:WP_011842153.1 222 QDEHPR-ATTPEALAKLKPLF-AGGSVTAGNSSGVNDGAAALILASEAAVRRHGLTPIARVLGGATAGVPPRI 292 ******.7999********98.689************************************************ PP TIGR02430 294 mGlgpvpavkkllaraglsledldvielneafaaqalavlrelgladddarvnpnGGaialGhplGasGarlv 366 mG+gpvpa+ kl+ar+gl+ e +dvielneafaaq++a+lr+lg+a++darvnpnGGaialGhplG+sGar+ NCBI__GCF_000015985.1:WP_011842153.1 293 MGIGPVPAALKLMARLGLTTEAFDVIELNEAFAAQGVATLRQLGIAEEDARVNPNGGAIALGHPLGMSGARIT 365 ************************************************************************* PP TIGR02430 367 ltalkqleksggryalatlciGvGqGialvierv 400 ta ql+++ggr+al+++ciGvGqGia+++erv NCBI__GCF_000015985.1:WP_011842153.1 366 GTAALQLQRTGGRRALSAMCIGVGQGIAIALERV 399 *********************************8 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (400 nodes) Target sequences: 1 (399 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.00u 0.00s 00:00:00.00 Elapsed: 00:00:00.00 # Mc/sec: 28.97 // [ok]
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory