Alignments for a candidate for gabD in Cereibacter sphaeroides ATCC 17029

GapMind for catabolism of small carbon sources

Alignments for a candidate for gabD in Cereibacter sphaeroides ATCC 17029

Align 4-(gamma-glutamylamino)butanal dehydrogenase (EC 1.2.1.99) (characterized)
to candidate WP_011842384.1 RSPH17029_RS17490 aldehyde dehydrogenase family protein

Query= BRENDA::P23883
         (495 letters)



>NCBI__GCF_000015985.1:WP_011842384.1
          Length = 791

 Score =  293 bits (751), Expect = 1e-83
 Identities = 175/469 (37%), Positives = 264/469 (56%), Gaps = 20/469 (4%)

Query: 23  FINGEYTAAAENETFETVDPVTQAPLAKIARGKSVDIDRAMSAARGVFERGDWSLSSPAK 82
           +I+G +T A E    E  +P T   LA+I      +I+ A++AAR  F    WS     +
Sbjct: 36  YIDGAFTGA-ETAAIEVENPATGEILARIPAAGEAEIEAAVAAARAAFS--GWSQLPGFE 92

Query: 83  RKAVLNKLADLMEAHAEELALLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEV 142
           R   L  +A  ++      ++LETLD GK IR +   D+P A R    +A     V GE 
Sbjct: 93  RARYLYAIARGLQKRERFFSVLETLDNGKAIRETRTADVPLAIRHFYHHA-GWAAVLGEE 151

Query: 143 ATTSSHELAMIVREPVGVIAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIR 202
                        EP+GV   ++PWNFP+L+  WK+ PALAAGN+V+LKP++ +PL+A+ 
Sbjct: 152 FPG---------HEPLGVCGQVIPWNFPMLMLAWKIAPALAAGNTVVLKPADLTPLTAVA 202

Query: 203 LAGLAKEAGLPDGVLNVVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMK 262
            A +  E GLP GV+N+V G G E G  L RH  +  +AFTGST  G+++ +    S  K
Sbjct: 203 FAEMLDEIGLPRGVVNIVHG-GAETGALLVRHPGVAKVAFTGSTAVGREIRRATAGSG-K 260

Query: 263 RVWLEAGGKSANIVFADCPDLQQAASATAAGIFYNQGQVCIAGTRLLLEESIADEFLALL 322
            + LE GGKS  +V AD  DL  A      G+++NQG+VC AG+RLLL+E IA+ FLA L
Sbjct: 261 SLTLELGGKSPFVVCADA-DLDAAVEGVVEGVWFNQGEVCCAGSRLLLQEGIAERFLAKL 319

Query: 323 KQQAQNWQPGHPLDPATTMGTLIDCAHADSVHSFIREGESKGQLLLDGRNAGLAAA---I 379
           + + +  + G PLD +T MG ++       +   I  G ++    L+     L AA   +
Sbjct: 320 RARMEKIRVGDPLDKSTDMGAIVSARQKARIEELIA-GAAREGYRLEQAACPLPAAGHFV 378

Query: 380 GPTIFVDVDPNASLSREEIFGPVLVVTRFTSEEQALQLANDSQYGLGAAVWTRDLSRAHR 439
            P  F D +P A++++ EIFGP+ V T F + ++A+ LAN++ YGL A+VW+ +++ A  
Sbjct: 379 APGFFADTEPAATVAQVEIFGPIAVTTTFRTVDEAVALANNTPYGLAASVWSENINAATE 438

Query: 440 MSRRLKAGSVFVNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKT 488
           ++ R++AG V++N  N  D    FGG K+SG GR+ +   L  +   +T
Sbjct: 439 LAARIRAGVVWINASNLFDAGASFGGMKESGFGREGAREGLGAYLRPRT 487



 Score = 89.7 bits (221), Expect = 3e-22
 Identities = 66/212 (31%), Positives = 102/212 (48%), Gaps = 7/212 (3%)

Query: 58  DIDRAMSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELALLETLDTGKPIRHSL 117
           DI  A+ AA    +   W+ ++   R  VL  LA+ + A AE+LA    L  G   R   
Sbjct: 555 DIRNAVEAA---LKAKGWAANAHG-RAQVLFFLAENIAARAEDLAA--ALVQGGAGRSEA 608

Query: 118 RDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGVIAAIVPWNFPLLLTCWK 177
             ++      + +YA   DK  G +  T    L + V+EP+GV+  + P   PLL     
Sbjct: 609 AAEVRSLIERVFFYAGMADKDDGRIHATKPRHLTLSVKEPLGVVGVLAPDEAPLLSLMSL 668

Query: 178 LGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLNVVTGFGHEAGQALSRHNDI 237
           + P +AAGN V+  PS    L A  L  +   + LP GV+N+VTG  +   + L+ H+ +
Sbjct: 669 ILPLIAAGNRVVAVPSPAQALLAQPLTQIFDTSDLPGGVVNLVTGDRNLLARTLAEHDAV 728

Query: 238 DAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAG 269
           D I + GS + G   ++     N+K+VW   G
Sbjct: 729 DGIWYHGSAK-GAAEVEALSAGNLKQVWTNGG 759


Lambda     K      H
   0.317    0.133    0.389 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 943
Number of extensions: 54
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 495
Length of database: 791
Length adjustment: 37
Effective length of query: 458
Effective length of database: 754
Effective search space:   345332
Effective search space used:   345332
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources