Align Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized)
to candidate WP_011842384.1 RSPH17029_RS17490 aldehyde dehydrogenase family protein
Query= SwissProt::Q9I6M5 (483 letters) >NCBI__GCF_000015985.1:WP_011842384.1 Length = 791 Score = 298 bits (764), Expect = 4e-85 Identities = 171/462 (37%), Positives = 259/462 (56%), Gaps = 16/462 (3%) Query: 14 YVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKALPAWRALTAKERA 73 Y+DGA+ A+ I+V NPATGEI+ +P G AE A+ AA A W L ERA Sbjct: 36 YIDGAFTGAETA-AIEVENPATGEILARIPAAGEAEIEAAVAAARAAFSGWSQLPGFERA 94 Query: 74 NKLRRWFDLMIENQDDLARLMTIEQGKPLAEAK-GEIAYAAS-FLEWFGEEAKRIYGDTI 131 L + + + + L T++ GK + E + ++ A F G A + G+ Sbjct: 95 RYLYAIARGLQKRERFFSVLETLDNGKAIRETRTADVPLAIRHFYHHAGWAA--VLGEEF 152 Query: 132 PGHQPDKRIIVIKQPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKPASQTPYSALA 191 PGH+P +GV + PWNFP M+ K PALAAG T+VLKPA TP +A+A Sbjct: 153 PGHEP----------LGVCGQVIPWNFPMLMLAWKIAPALAAGNTVVLKPADLTPLTAVA 202 Query: 192 LAELAERAGIPKGVFSVVTGSAGEVGGELTSNPIVRKLTFTGSTEIGRQLMAECAQDIKK 251 AE+ + G+P+GV ++V G A E G L +P V K+ FTGST +GR++ A K Sbjct: 203 FAEMLDEIGLPRGVVNIVHGGA-ETGALLVRHPGVAKVAFTGSTAVGREIRRATAGSGKS 261 Query: 252 VSLELGGNAPFIVFDDADLDAAVEGALISKYRNNGQTCVCANRLYVQDGVYDAFVDKLKA 311 ++LELGG +PF+V DADLDAAVEG + + N G+ C +RL +Q+G+ + F+ KL+A Sbjct: 262 LTLELGGKSPFVVCADADLDAAVEGVVEGVWFNQGEVCCAGSRLLLQEGIAERFLAKLRA 321 Query: 312 AVAKLNIGNGLEAGVTTGPLIDAKAVAKVEEHIADAVSKGAKVVSGGKPHALGGTFFEPT 371 + K+ +G+ L+ G ++ A+ A++EE IA A +G ++ P G F P Sbjct: 322 RMEKIRVGDPLDKSTDMGAIVSARQKARIEELIAGAAREGYRLEQAACPLPAAGHFVAPG 381 Query: 372 ILVDVPKNALVSKDETFGPLAPVFRFKDEAEVIAMSNDTEFGLASYFYARDLARVFRVAE 431 D A V++ E FGP+A F+ E +A++N+T +GLA+ ++ ++ +A Sbjct: 382 FFADTEPAATVAQVEIFGPIAVTTTFRTVDEAVALANNTPYGLAASVWSENINAATELAA 441 Query: 432 QLEYGMVGINTGLISNEVAPFGGIKASGLGREGSKYGIEDYL 473 ++ G+V IN + + A FGG+K SG GREG++ G+ YL Sbjct: 442 RIRAGVVWINASNLFDAGASFGGMKESGFGREGAREGLGAYL 483 Score = 76.6 bits (187), Expect = 3e-18 Identities = 65/239 (27%), Positives = 109/239 (45%), Gaps = 5/239 (2%) Query: 14 YVDGAWVDADNGQTIKVNNPATGEIIGSVPKMGAAETRRAIEAADKALPAWRALTAKERA 73 Y+ GA V D G + V P GE +G P G + R A+EAA KA W A A RA Sbjct: 521 YIGGAQVRPDGGASYVVRGPK-GEALGLAPVSGRKDIRNAVEAALKA-KGWAA-NAHGRA 577 Query: 74 NKLRRWFDLMIENQDDLARLMTIEQGKPLAEAKGEIAYAASFLEWFGEEAKRIYGDTIPG 133 L + + +DLA + ++ G +EA E+ + ++ A + G I Sbjct: 578 QVLFFLAENIAARAEDLAAAL-VQGGAGRSEAAAEVRSLIERVFFYAGMADKDDG-RIHA 635 Query: 134 HQPDKRIIVIKQPIGVTAAITPWNFPSAMITRKAGPALAAGCTMVLKPASQTPYSALALA 193 +P + +K+P+GV + P P + P +AAG +V P+ A L Sbjct: 636 TKPRHLTLSVKEPLGVVGVLAPDEAPLLSLMSLILPLIAAGNRVVAVPSPAQALLAQPLT 695 Query: 194 ELAERAGIPKGVFSVVTGSAGEVGGELTSNPIVRKLTFTGSTEIGRQLMAECAQDIKKV 252 ++ + + +P GV ++VTG + L + V + + GS + ++ A A ++K+V Sbjct: 696 QIFDTSDLPGGVVNLVTGDRNLLARTLAEHDAVDGIWYHGSAKGAAEVEALSAGNLKQV 754 Lambda K H 0.317 0.135 0.391 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 910 Number of extensions: 50 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 483 Length of database: 791 Length adjustment: 37 Effective length of query: 446 Effective length of database: 754 Effective search space: 336284 Effective search space used: 336284 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Apr 10 2024. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory