Alignments for a candidate for ech in Burkholderia vietnamiensis G4

GapMind for catabolism of small carbon sources

Alignments for a candidate for ech in Burkholderia vietnamiensis G4

Align Peroxisomal bifunctional enzyme; PBE; PBFE; EC 4.2.1.17; EC 5.3.3.8; EC 1.1.1.35 (characterized)
to candidate WP_011886130.1 3-hydroxyacyl-CoA dehydrogenase

Query= SwissProt::Q08426
         (723 letters)



>NCBI__GCF_000016205.1:WP_011886130.1
          Length = 711

 Score =  500 bits (1288), Expect = e-146
 Identities = 295/703 (41%), Positives = 412/703 (58%), Gaps = 28/703 (3%)

Query: 9   NALALIRLRNPPVNAISTTLLRDIKEGLQKAVIDHTIKAIVICGAEGKFSAGADIRGFSA 68
           +AL ++ + +PPVNA+S  + R + + L  A  DH ++A++I GA   F AGADIR F  
Sbjct: 21  DALLVVTIDHPPVNALSADVRRGLADALDAAQADHAVRAVLIVGAGRNFIAGADIREFGK 80

Query: 69  PRTFGLTLGHVVDEIQRNEKPVVAAIQGMAFGGGLELALGCHYRIAHAEAQVGLPEVTLG 128
           P     +L  V + I+ + KPVV A+ G   GGGLE+AL  HYR+A   A++GLPEVTLG
Sbjct: 81  PPV-PPSLPDVCERIESSAKPVVVALHGATLGGGLEVALAAHYRLALPGAKLGLPEVTLG 139

Query: 129 LLPGARGTQLLPRLTGVPAALDLITSGRRILADEALKLGILDKVVNSDP-VEEAIRFAQR 187
           LLPGA GTQ  PRL G  AALDL+ +GR   A+EA  LG++D+V  SD  + E + +AQ 
Sbjct: 140 LLPGAGGTQRTPRLIGAKAALDLMLTGRHASAEEARALGLVDRVARSDDTLAEGLAYAQE 199

Query: 188 VSDQPLESRRLCNKPIQSLPNM---DSIFSEALLKMRRQHPGCLAQEACVRAVQAAVQYP 244
           +       RR   +  Q L       +  + A  ++ ++  G  +    V AV+AA+   
Sbjct: 200 LVSLGAPVRR--TRDAQGLAERTAAQAALAAARAELPKKSRGLFSPAKIVDAVEAALTLS 257

Query: 245 YEVGIKKEEELFLYLLQSGQARALQYAFFAERKANKWSTPSGASWKTASARPVSSVGVVG 304
           ++ G+K E  LFL  L S Q   L +AFFAER+A K         + ASAR V  VGVVG
Sbjct: 258 FDAGMKLERSLFLQCLDSPQRAGLVHAFFAEREAAK-----APEARRASARAVERVGVVG 312

Query: 305 LGTMGRGIVISFARARIPVIAVDSDKNQLATANKMITSVLEKEASKMQQSGHPWSGPKPR 364
            GTMG GI ++   A +PV  ++ D   LA     +  V +   +K + +    +    R
Sbjct: 313 GGTMGAGIAVAALDAGLPVTMIERDDASLARGRAHVEKVYDALVAKGRMTPDARAARVAR 372

Query: 365 LTSSVK--ELGGVDLVIEAVFEEMSLKKQVFAELSAVCKPEAFLCTNTSALDVDEIASST 422
           L  S +   L   D+VIEAVFE+M++K+ VFAEL+ VCKP A L TNTS LD+D +A+  
Sbjct: 373 LDGSTRYDALAQADVVIEAVFEDMAVKQAVFAELARVCKPGAVLATNTSYLDIDALAAGI 432

Query: 423 DRPHLVIGTHFFSPAHVMKLLEVIPSQYSSPTTIATVMNLSKKIKKIGVVVGNCFGFVGN 482
           DRP  VIG HFFSPA+VMKLLE++  +  S   +AT   L++++KK  V  G C GF+GN
Sbjct: 433 DRPTDVIGLHFFSPANVMKLLEIVVPKRVSAEVVATAFALARQLKKTPVRAGVCDGFIGN 492

Query: 483 RMLNPYYNQAYFLLEEGSKPEEVDQVLEEFGFKMGPFRVSDLAGLDVGWKSRKGQGLTGP 542
           R+L  Y   A +L+E+G+ P ++D+ +  FGF MGPF+V DLAG D+GW +RK +  T  
Sbjct: 493 RILAVYRTAADYLMEDGASPYQIDRAVRGFGFPMGPFQVVDLAGGDIGWATRKRRAAT-- 550

Query: 543 TLLPGTPARKRGNRRYCPIPDVLCELGRFGQKTGKGWYQYDKPLGRIHKPDPWLSKFLSR 602
                    +    RY  I D LCE G FGQK+G+G+Y Y     R+  PDP +   ++ 
Sbjct: 551 ---------RDPRARYVEISDRLCERGWFGQKSGRGYYLYPDG-ARVGTPDPEVDAIVAA 600

Query: 603 YRKTHHIEPRTISQDEILERCLYSLINEAFRILGEGIAASPEHIDVVYLHGYGWPRHKGG 662
            R    I PR+ S DEIL R L ++INE   ++ E IA  P  +D V+L+GYG+PRH+GG
Sbjct: 601 ERAKKGIVPRSFSDDEILRRYLAAMINEGANVVHERIALRPLDVDAVFLYGYGFPRHRGG 660

Query: 663 PMFYASTVGLPTVLEKLQKYYRQNPDIPQLEPSDYLKKLASQG 705
           PM YA TVGL  VL  ++ +  ++P      PS  L +L  +G
Sbjct: 661 PMHYADTVGLANVLADIRAFANEDPLF--WRPSPLLVELVERG 701


Lambda     K      H
   0.319    0.136    0.405 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1115
Number of extensions: 53
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 723
Length of database: 711
Length adjustment: 40
Effective length of query: 683
Effective length of database: 671
Effective search space:   458293
Effective search space used:   458293
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources