Align L-lactate permease (characterized, see rationale)
to candidate WP_011911672.1 PST_RS02255 L-lactate permease
Query= uniprot:L0GFN1 (564 letters) >NCBI__GCF_000013785.1:WP_011911672.1 Length = 567 Score = 774 bits (1998), Expect = 0.0 Identities = 373/564 (66%), Positives = 460/564 (81%), Gaps = 1/564 (0%) Query: 1 MSNGLLALFAFTPILLAAIMLIGLRWPASRAMPLVFLFTAAIGLFVWDMSVNRIIASTLQ 60 MS LL++ AF P++LA ++LIG RWPA AMPLVF+ TA IGL WDM++NR+IAST Q Sbjct: 1 MSQTLLSILAFVPLVLAGVLLIGFRWPAKYAMPLVFVLTALIGLLAWDMTLNRVIASTFQ 60 Query: 61 GLVITLGLLWIIFGAILLLNTLKHSGGITAIRAGFTTISPDRRIQAIIIAWLFGCFIEGA 120 GL++T +LWIIFGAILLLNTLKHSGGI++IR GF+ ISPDRR+Q +I+AWLFGCFIEGA Sbjct: 61 GLILTAAILWIIFGAILLLNTLKHSGGISSIRRGFSNISPDRRVQVLIVAWLFGCFIEGA 120 Query: 121 SGFGTPAAIAAPLLVAVGFPAMAAVLLGMLVQSTPVSFGAVGTPIVVGINSGLDTATIGA 180 SGFGTPAA+AAPL+VA+GFPA+AAV++GM+VQSTPVSFGAVGTPI+VG+ +GLD I Sbjct: 121 SGFGTPAAVAAPLMVALGFPALAAVVMGMMVQSTPVSFGAVGTPILVGVGAGLDKTGISE 180 Query: 181 QLVAQGSSWNAYLQQITSSVAITHAIVGTVMPLVMVLMLTRFFGKEKSWKAGFEVLPFAI 240 QL A GS+W + I S VAI HA+ G +MPL+MV ++TR+FG+ KSW G + PFA+ Sbjct: 181 QLAAVGSNWEVFFHLIFSRVAIIHALCGILMPLIMVSIMTRYFGRNKSWTEGLAIAPFAV 240 Query: 241 FAGLAFTLPYAATGIFLGPEFPSLLGGLVGLAIVTTAARFKFLTPKTTWDFADAKEWPAE 300 F GLAF +PYAA G+FLGPEFPS++G LVGLAIV AA+ FL PK +WDFA A EWP+E Sbjct: 241 FTGLAFVIPYAAAGVFLGPEFPSMIGALVGLAIVVPAAKAGFLLPKDSWDFAPASEWPSE 300 Query: 301 WLGTIEMKLDEMAAR-PMSAFRAWLPYVLVGAILVISRVFPQVTAALKSVSIAFANILGE 359 W+G IEMK++++A + P+S AW PY+++ +LV SRVFP A L S+S + +ILGE Sbjct: 301 WMGKIEMKIEDVAGKTPISVPMAWAPYLILAGLLVASRVFPDFKALLMSLSFGWKDILGE 360 Query: 360 TGINAGIEPLYLPGGILVMVVLITFFLHGMRVSELKAAVKESSGVLLSAGFVLLFTVPMV 419 TG++ +EPLYLPGGIL +VVLITFFLH M EL AA+ ESS LL AGFVL+FT+PMV Sbjct: 361 TGVSGTLEPLYLPGGILCIVVLITFFLHRMSARELGAAISESSKTLLGAGFVLIFTIPMV 420 Query: 420 RILINSGVNGAELASMPIVMARYVADSVGSIYPLLAPAVGALGAFLAGSNTVSNMMFSQF 479 RILINSGVNG++L SMP+ MA+ VA+SVG +YP APAVGALGAF+AGSNTVSN+M SQF Sbjct: 421 RILINSGVNGSDLMSMPVAMAQLVANSVGDVYPFFAPAVGALGAFIAGSNTVSNLMLSQF 480 Query: 480 QFGVAQSLGISGAMVVATQAVGAAAGNMVAIHNVVAASATVGLLGREGSTLRKTIWPTLY 539 QF A LG+SGA++VA Q+VGAAAGNM+AIHNVVAASATVGLLGREG TLRKT+ PTLY Sbjct: 481 QFNTAGLLGLSGALMVALQSVGAAAGNMIAIHNVVAASATVGLLGREGITLRKTMLPTLY 540 Query: 540 YVLFTGVIGLIAIYVLGVTDPLVG 563 Y++ G IGLI YV+G++DPLVG Sbjct: 541 YLILAGTIGLIGFYVMGISDPLVG 564 Lambda K H 0.326 0.140 0.415 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1156 Number of extensions: 54 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 564 Length of database: 567 Length adjustment: 36 Effective length of query: 528 Effective length of database: 531 Effective search space: 280368 Effective search space used: 280368 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 15 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory