Align 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27) (characterized)
to candidate WP_011912059.1 PST_RS04445 4-hydroxyphenylpyruvate dioxygenase
Query= reanno::psRCH2:GFF3449 (361 letters) >NCBI__GCF_000013785.1:WP_011912059.1 Length = 361 Score = 711 bits (1836), Expect = 0.0 Identities = 349/361 (96%), Positives = 352/361 (97%) Query: 1 MNAVNKIEQHNPIGTDGFEFVEFTAPNAEGIEQLRTLFTQMGFTETAKHRSKEVWLFQQH 60 MNAVNKIEQHNPIGTDGFEFVEFTAPNAEGIEQLRTLFTQMGFTETAKHRSKEVWLFQQH Sbjct: 1 MNAVNKIEQHNPIGTDGFEFVEFTAPNAEGIEQLRTLFTQMGFTETAKHRSKEVWLFQQH 60 Query: 61 DINIVLNGSPTGHVHAFAEKHGPSACAMAFRVKNAAQAAAYVESQGAKLVGSHANFGELN 120 DINIVLNGSPTGHV F EKHGPSACAMAFRVKNAAQAAAYVESQGAKLVGSHANFGELN Sbjct: 61 DINIVLNGSPTGHVREFGEKHGPSACAMAFRVKNAAQAAAYVESQGAKLVGSHANFGELN 120 Query: 121 IPCVEGIGGSLLYLVDRYGDKSIYDVDFEYIEGRTPNDNAVGLMCIDHLTHNVMRGQMDV 180 IPCVEGIGGSLLYLVDRYGDKSIYDVDFEYIEGRTPNDNAVGLMCIDHLTHNV RGQMDV Sbjct: 121 IPCVEGIGGSLLYLVDRYGDKSIYDVDFEYIEGRTPNDNAVGLMCIDHLTHNVRRGQMDV 180 Query: 181 WSGFYERIANFREIRYFDIEGKLTGLFSRAMTAPCGKIRIPINESADDKSQIEEFIREYH 240 WSGFYERIANFREIRYFDIEGKLTGLFSRAMTAPCGKIRIPINESADDKSQIEEFIREYH Sbjct: 181 WSGFYERIANFREIRYFDIEGKLTGLFSRAMTAPCGKIRIPINESADDKSQIEEFIREYH 240 Query: 241 GEGIQHIALSTDDIYATVRQLRANGVDFMTTPDTYYEKVDTRVAGHGEPTDVLRELNILI 300 GEGIQHIALSTDDIY TVR+LRANGVDFMTTP TYYEKVDTRVAGHGEP D LRELN+LI Sbjct: 241 GEGIQHIALSTDDIYETVRRLRANGVDFMTTPGTYYEKVDTRVAGHGEPLDQLRELNLLI 300 Query: 301 DGAPGDDGILLQIFTNTVIGPIFFEIIQRKGNQGFGEGNFKALFESIEEDQLRRGVIKAD 360 DGAPGDDGILLQIFTNTVIGPIFFEIIQRKGNQGFGEGNFKALFESIEEDQ+RRGVIKA Sbjct: 301 DGAPGDDGILLQIFTNTVIGPIFFEIIQRKGNQGFGEGNFKALFESIEEDQVRRGVIKAG 360 Query: 361 E 361 E Sbjct: 361 E 361 Lambda K H 0.320 0.139 0.412 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 698 Number of extensions: 15 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 361 Length of database: 361 Length adjustment: 29 Effective length of query: 332 Effective length of database: 332 Effective search space: 110224 Effective search space used: 110224 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 49 (23.5 bits)
Align candidate WP_011912059.1 PST_RS04445 (4-hydroxyphenylpyruvate dioxygenase)
to HMM TIGR01263 (hppD: 4-hydroxyphenylpyruvate dioxygenase (EC 1.13.11.27))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR01263.hmm # target sequence database: /tmp/gapView.15506.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR01263 [M=353] Accession: TIGR01263 Description: 4HPPD: 4-hydroxyphenylpyruvate dioxygenase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 1e-126 409.1 0.0 1.2e-126 409.0 0.0 1.0 1 lcl|NCBI__GCF_000013785.1:WP_011912059.1 PST_RS04445 4-hydroxyphenylpyruv Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000013785.1:WP_011912059.1 PST_RS04445 4-hydroxyphenylpyruvate dioxygenase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 409.0 0.0 1.2e-126 1.2e-126 2 352 .. 17 356 .. 16 357 .. 0.97 Alignments for each domain: == domain 1 score: 409.0 bits; conditional E-value: 1.2e-126 TIGR01263 2 gfdfvefavgdak..qaakalveklGfeavaketgsrekastvlrqgeitlvltaelsssseaaaflak 68 gf+fvef++++a+ +++++l++++Gf++ ak +r+k++++++q +i++vl+ +++ + + +f +k lcl|NCBI__GCF_000013785.1:WP_011912059.1 17 GFEFVEFTAPNAEgiEQLRTLFTQMGFTETAK---HRSKEVWLFQQHDINIVLNGSPTGH--VREFGEK 80 8***********988899*************9...************************9..******* PP TIGR01263 69 HGdgvkdvafevedveaafeaavergaeavsapeeedekevklaaikgiGdvvltlveregekgsilpg 137 HG++++++af+v+++++a + ++ga+ v ++ + +e++++ ++giG+++l+lv+r+g+k si+++ lcl|NCBI__GCF_000013785.1:WP_011912059.1 81 HGPSACAMAFRVKNAAQAAAYVESQGAKLVGSHAN--FGELNIPCVEGIGGSLLYLVDRYGDK-SIYDV 146 *****************************999985..799*********************99.9**** PP TIGR01263 138 feevsekaalkekledvgleaiDHvvgnvergelekvaefyekilgfkeiksfdikteasaLkSkvlas 206 ++e e + ++ + vgl iDH+++nv+rg+++ ++ fye+i +f+ei++fdi+++ ++L S+++++ lcl|NCBI__GCF_000013785.1:WP_011912059.1 147 DFEYIEGRTPND--NAVGLMCIDHLTHNVRRGQMDVWSGFYERIANFREIRYFDIEGKLTGLFSRAMTA 213 **9999865555..89***************************************************** PP TIGR01263 207 aegkvklplnepaskkkksQIeeyleeyeGaGvQHlAlntedivktveelrargveflkipetYYdnlk 275 ++gk+++p+ne s ++ksQIee+++ey+G+G+QH+Al+t+di++tv++lra+gv+f+++p tYY++++ lcl|NCBI__GCF_000013785.1:WP_011912059.1 214 PCGKIRIPINE--SADDKSQIEEFIREYHGEGIQHIALSTDDIYETVRRLRANGVDFMTTPGTYYEKVD 280 ***********..89****************************************************** PP TIGR01263 276 ervkklvkedleelkelkiLvDrd..eeGlLLQiFtkpvvdrgtlFfEiIqRkgakGFGegNfkaLfea 342 rv + e+l++l+el++L+D++ ++G+LLQiFt++v+ g++FfEiIqRkg++GFGegNfkaLfe+ lcl|NCBI__GCF_000013785.1:WP_011912059.1 281 TRVAG-HGEPLDQLRELNLLIDGApgDDGILLQIFTNTVI--GPIFFEIIQRKGNQGFGEGNFKALFES 346 ****7.**********************************..*************************** PP TIGR01263 343 iEreqekrgv 352 iE++q++rgv lcl|NCBI__GCF_000013785.1:WP_011912059.1 347 IEEDQVRRGV 356 ********98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (353 nodes) Target sequences: 1 (361 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01 # Mc/sec: 11.55 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory