Alignments for a candidate for paaK in Sphingomonas wittichii RW1

GapMind for catabolism of small carbon sources

Alignments for a candidate for paaK in Sphingomonas wittichii RW1

Align phenylacetate-CoA ligase (EC 6.2.1.30) (characterized)
to candidate WP_011952290.1 SWIT_RS07325 AMP-binding protein

Query= BRENDA::D3GE78
         (556 letters)



>NCBI__GCF_000016765.1:WP_011952290.1
          Length = 596

 Score =  168 bits (426), Expect = 5e-46
 Identities = 144/522 (27%), Positives = 229/522 (43%), Gaps = 42/522 (8%)

Query: 46  LTTHDLRLWSQRLAAGLRKSGLQRGDRVLLFSGNDLFFPVVFLGVIMAGGIFTGANPTFV 105
           L+  DL   +   AAGL   GL  GDR+ ++S N + + +       AG +    NP + 
Sbjct: 46  LSWRDLARRAGDFAAGLLALGLAPGDRIGIWSLNRVEWAITQFAAAKAGLVLVTINPNYR 105

Query: 106 ARELAYQLQDSGATYLLCASNSLETGLEAAKQAKLPQS---------------HIFAYDT 150
             EL Y L+  G   ++ AS    +      +  +P+                H+   D 
Sbjct: 106 LSELEYALRSVGCAAVVTASVFKSSDYVGMLRTLMPEMAAGPGFVAERLPALRHVIQMDP 165

Query: 151 SIYDGVTNPQKGCAYWSDLLASEEEGAAFTWDELSTPALSSTTLALNYSSGTTGRPKGVE 210
                   P  G   ++ + A   +        L     S   + + ++SGTTG PKGV 
Sbjct: 166 E-------PVAGAVAFATVEALGRDAGPARLRALEGVLGSRDPVNIQFTSGTTGLPKGVT 218

Query: 211 ISHRNYVANMLQYCHTASLHPDYKARLERSRWLCF-LPMYHAMAQNIF-IAAALYRATPV 268
           +SHRN + N              K +L     +C  +P+YH     +  +AA  + A  V
Sbjct: 219 LSHRNILNN--------GYFTGLKLKLTAQDRICIPVPLYHCFGMVMGNLAAVTHGAAMV 270

Query: 269 YIMSKFDFVKMLEYTQRFRITDFILVPPVVVALAKHPAVGQYDLSSVELVGSGAAPLGRE 328
           Y    FD +  L      R T    VP + +A  +HPA   +DLSS+       +P   E
Sbjct: 271 YPGEGFDPLATLAALSGERCTALYGVPTMFIAQLEHPAFAAHDLSSLRTGIMAGSPCPIE 330

Query: 329 VCEEVEKLWPPGKINIKQGWGMTEATC----SVTGWNPAEISTSASVGELNANCEAKIMF 384
           V  +        +I I   +GMTE +     S T  +P E+    +VG ++ + E KI+ 
Sbjct: 331 VMRKAIDRMNLREITIC--YGMTETSPVSFQSETD-DPVELRVG-TVGRVHPHLEVKIVD 386

Query: 385 DGVEVKERNSRGELWVRAPNVMKGYWRNEKATKETKTEDGWLLTGDIAFVDDDGKFHVVD 444
           D  E   R   GEL  R  +VM GYW + + T     + GW+ +GD+A +D+DG   +V 
Sbjct: 387 DAGETVPRGVAGELCTRGYSVMLGYWNDPEQTAAAIDDAGWMHSGDLATIDEDGYCRIVG 446

Query: 445 RMKELIKVKGNQVAPAELEALLLEHPAISDVAVIGVVINN-DERPRAYVVLRPGQSATAN 503
           R+K+++   G  + P E+E  L  HPAI DVAV GV   +  E   A+++   G      
Sbjct: 447 RIKDMVIRGGENLYPREIEEFLHTHPAIRDVAVFGVPDRHYGEELCAWIIRHDGAPIDET 506

Query: 504 EIAHYLDNKVSAFKRITGGVVFLEAIPKNPSGKILRMKLREQ 545
           E+  +    +S  ++I   + F++A P   +GK+ +  +REQ
Sbjct: 507 EVRAFCRGHIS-HQKIPRHIRFVDAFPLTVTGKVQKFVMREQ 547


Lambda     K      H
   0.319    0.134    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 672
Number of extensions: 41
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 556
Length of database: 596
Length adjustment: 36
Effective length of query: 520
Effective length of database: 560
Effective search space:   291200
Effective search space used:   291200
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources