Alignments for a candidate for xacD in Xanthobacter autotrophicus Py2

GapMind for catabolism of small carbon sources

Alignments for a candidate for xacD in Xanthobacter autotrophicus Py2

Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_011995749.1 XAUT_RS00355 dihydroxy-acid dehydratase

Query= reanno::Smeli:SM_b20890
         (579 letters)



>NCBI__GCF_000017645.1:WP_011995749.1
          Length = 617

 Score =  256 bits (655), Expect = 1e-72
 Identities = 192/573 (33%), Positives = 293/573 (51%), Gaps = 71/573 (12%)

Query: 21  GKNAIMHRSWMKNQGLPADTFDGRPIIGICNTWSELTPCNAHLRDLAERVKRGVYEAGGF 80
           G+N    R   +  G+    F G+PII + N++++  P + HL+DL + V R + +AGG 
Sbjct: 12  GRNMAGARGLWRATGMKDGDF-GKPIIAVVNSFTQFVPGHVHLKDLGQLVAREIEKAGGV 70

Query: 81  PVEFPVFSTGES-TLRPTAMMF----RNLAAMDVEESIRGNPVDGVVLLGGCDKTTPSLL 135
             EF   +  +   +    M++    R + A  VE  +  +  D +V +  CDK TP +L
Sbjct: 71  AKEFNTIAVDDGIAMGHDGMLYSLPSREIIADSVEYMVNAHCADAMVCISNCDKITPGML 130

Query: 136 MGAASVDIPAIVVSGGPMLNGKWRGKDVGSGTA-IWQFSEMVKSGEMSLEEFMDAE-QGM 193
           M A  ++IPA+ VSGGPM  GK     + +G   +     M+ + +   ++  DA+ Q M
Sbjct: 131 MAAMRLNIPAVFVSGGPMEAGKVL---LSTGEKKVDLIDAMIAAAD---DKVTDADVQVM 184

Query: 194 ARSA----GSCMTMGTASTMASMAEALGMTLSGNAAIPAVDARRRVISQLTGRRIVEMVK 249
            RSA    GSC  M TA++M  + EALG+ L GN +  A  A R  +    G  IV++ +
Sbjct: 185 ERSACPTCGSCSGMFTANSMNCLTEALGLALPGNGSTLATHADRERLFVEAGHLIVDLAR 244

Query: 250 E-------DLKPSDILTKEAFENAIRVNGAVGGSTNAVLHLLALAGRVGVDLSLDDWDRL 302
                    + P  + + +AFENA+ ++ ++GGSTN VLHLLA A    V  ++ D DRL
Sbjct: 245 RYYEQDDASVLPRSVASFKAFENAMTLDISMGGSTNTVLHLLAAANEGEVPFTMADIDRL 304

Query: 303 GRDVPTIVNLQPS-GKYLMEEFYYAGGLPVVIKAVAEMGLLHNDAITVSGDTI------W 355
            R VP +  + P+     ME+ + AGG+  ++  +   GL+H D  TV   T+      W
Sbjct: 305 SRRVPVLCKVAPAVANIHMEDVHRAGGIMAILGELDRGGLIHTDLPTVHAATMADALDRW 364

Query: 356 NDVKGVVNYN------------------------EDVILPRE--------KALTKSGGIA 383
            DVK   + +                        +D+ L RE         A +K GG+A
Sbjct: 365 -DVKRTKSESVATFFRAAPGGVPTQVAFSQSRRWDDLDLDRETGVIRDVAHAYSKDGGLA 423

Query: 384 VLRGNLAPRGAVLKPSAASPHLMQHKGRAVVFESIEDYHARINREDLDIDETCIMVLKYC 443
           VL GN+A  G ++K +     +++  G+A +FES +D           I    +++++Y 
Sbjct: 424 VLYGNIALDGCIVKTAGVDASILKFTGKARIFES-QDAAVEAILSTGRIQAGDVVLIRYE 482

Query: 444 GPKGYPGMAEVGNMGLPPKVLK-KGITDMIR-ISDARMSGTAYGTVILHTAPEAAEGGPL 501
           GP+G PGM E   M  P   LK KG+      ++D R SG + G  I H +PEAAEGG +
Sbjct: 483 GPRGGPGMQE---MLYPTSYLKSKGLGKACALVTDGRFSGGSSGLSIGHVSPEAAEGGAI 539

Query: 502 ALVENGDLIEVDIPNRTLHLHVSDEELARRRAA 534
            LVE GD IE+DIPNR + + VSDE L++RRAA
Sbjct: 540 GLVEEGDTIEIDIPNRIIRVAVSDEVLSQRRAA 572


Lambda     K      H
   0.318    0.135    0.402 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 883
Number of extensions: 52
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 579
Length of database: 617
Length adjustment: 37
Effective length of query: 542
Effective length of database: 580
Effective search space:   314360
Effective search space used:   314360
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources