Align Phenylacetate-coenzyme A ligase; Phenylacetyl-CoA ligase; PA-CoA ligase; EC 6.2.1.30 (characterized)
to candidate WP_012043018.1 BBTA_RS13375 phenylacetate--CoA ligase
Query= SwissProt::Q9L9C1 (440 letters) >NCBI__GCF_000015165.1:WP_012043018.1 Length = 442 Score = 578 bits (1490), Expect = e-169 Identities = 284/434 (65%), Positives = 340/434 (78%), Gaps = 13/434 (2%) Query: 10 DLEPIEKASQDELRALQLERLKWSVRHAYENVPHYRKAFDAKGVHPDDLKSLADLAKFPF 69 D++ E+AS+DE+ ALQ +RL WS++HAY+NV HYR+AFD GVHP D + LADLAKFPF Sbjct: 15 DMDATERASRDEIMALQTKRLAWSLKHAYDNVAHYRRAFDTAGVHPSDFRELADLAKFPF 74 Query: 70 TAKGDLRDNYPFGMFAVPREKVARVHASSGTTGKPTVVGYTLKDIDTWATVVARSIRASG 129 T K DLRDNYPF MFAVPRE++ RVHASSGTTGKP VVGYT DI TW+ V+ARSIRA+G Sbjct: 75 TVKTDLRDNYPFNMFAVPREQLVRVHASSGTTGKPIVVGYTRNDIATWSHVMARSIRAAG 134 Query: 130 GRAGDMVHIAYGYGLFTGGLGAHYGAEKLGCTVVPMSGGQTEKQIQLIQDFKPDIIMVTP 189 GR+G ++H AYGYGLFTGGLG HYGAE+LGCTVVP+SGG TE+Q+QLI DF+PDII VTP Sbjct: 135 GRSGMIIHNAYGYGLFTGGLGVHYGAEELGCTVVPISGGMTERQVQLINDFRPDIITVTP 194 Query: 190 SYMLTVLDEMERMGIDPHQTSLKVGIFGAEPWTQAMRAAMEARAGIDAVDIYGLSEVMGP 249 SYML +LDE +R G+DP Q+SLK+GIFGAEPWT AMRA +E +DA DIYGLSEV+GP Sbjct: 195 SYMLAILDEFKRQGLDPRQSSLKIGIFGAEPWTNAMRAEIEDAFAMDATDIYGLSEVIGP 254 Query: 250 GVANECIEAKDGPVIWEDHFYPEIIDPHTGEVLPDGSEGELVFTTLTKEAMPVIRYRTRD 309 GVA EC+E KDG IWEDHFYPEI+DP TG+VLPDG GELVFT+LTKEA PVIRYRTRD Sbjct: 255 GVAQECLETKDGLHIWEDHFYPEIVDPDTGKVLPDGEMGELVFTSLTKEAFPVIRYRTRD 314 Query: 310 LTRLLPPTAR-SMRRMAKITGRSDDMLIIRGVNLFPTQVEELICKNPKLAPQYLLEVDKD 368 LTRLLP TAR MRRM K+TGRSDDM+I+RGVNLFP+Q+EE++ ++LE+ ++ Sbjct: 315 LTRLLPGTARPGMRRMEKVTGRSDDMIILRGVNLFPSQIEEILLTTEWCGGHFMLELTRE 374 Query: 369 GHMDTLTVKVEINP----EANVGRHPEQKEALAKELQHDIKTFIGVSAKVHVCEPFAIER 424 G MD LT+ E P E +G H A+ + IK IGV+AKV P +ER Sbjct: 375 GRMDELTIYAESRPEHWDETGLGAH-------AERISIHIKNTIGVTAKVKAVPPDTLER 427 Query: 425 VTIGKAKRVVDRRP 438 + GKAKR+ DRRP Sbjct: 428 -SAGKAKRIKDRRP 440 Lambda K H 0.318 0.137 0.406 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 700 Number of extensions: 18 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 440 Length of database: 442 Length adjustment: 32 Effective length of query: 408 Effective length of database: 410 Effective search space: 167280 Effective search space used: 167280 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 51 (24.3 bits)
Align candidate WP_012043018.1 BBTA_RS13375 (phenylacetate--CoA ligase)
to HMM TIGR02155 (paaF: phenylacetate-CoA ligase (EC 6.2.1.30))
# hmmsearch :: search profile(s) against a sequence database # HMMER 3.3.1 (Jul 2020); http://hmmer.org/ # Copyright (C) 2020 Howard Hughes Medical Institute. # Freely distributed under the BSD open source license. # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - # query HMM file: ../tmp/path.carbon/TIGR02155.hmm # target sequence database: /tmp/gapView.28462.genome.faa # - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - Query: TIGR02155 [M=422] Accession: TIGR02155 Description: PA_CoA_ligase: phenylacetate-CoA ligase Scores for complete sequences (score includes all domains): --- full sequence --- --- best 1 domain --- -#dom- E-value score bias E-value score bias exp N Sequence Description ------- ------ ----- ------- ------ ----- ---- -- -------- ----------- 3.9e-230 749.5 0.0 4.5e-230 749.3 0.0 1.0 1 lcl|NCBI__GCF_000015165.1:WP_012043018.1 BBTA_RS13375 phenylacetate--CoA Domain annotation for each sequence (and alignments): >> lcl|NCBI__GCF_000015165.1:WP_012043018.1 BBTA_RS13375 phenylacetate--CoA ligase # score bias c-Evalue i-Evalue hmmfrom hmm to alifrom ali to envfrom env to acc --- ------ ----- --------- --------- ------- ------- ------- ------- ------- ------- ---- 1 ! 749.3 0.0 4.5e-230 4.5e-230 1 422 [] 20 439 .. 20 439 .. 0.99 Alignments for each domain: == domain 1 score: 749.3 bits; conditional E-value: 4.5e-230 TIGR02155 1 eklsldelralqlerlkvsvkrayenvpayrkafdaagvkpddlkelsdlakfpltaksdlrdnypfdl 69 e++s+de++alq++rl +s+k+ay+nv++yr+afd agv+p+d++el+dlakfp+t+k+dlrdnypf++ lcl|NCBI__GCF_000015165.1:WP_012043018.1 20 ERASRDEIMALQTKRLAWSLKHAYDNVAHYRRAFDTAGVHPSDFRELADLAKFPFTVKTDLRDNYPFNM 88 799****************************************************************** PP TIGR02155 70 lavprekvvrvhassGttGkptvvaytqkdldtwsevvarslraaGGrkgdllhnayGyGlftGGlGvh 138 +avpre++vrvhassGttGkp+vv+yt++d+ tws+v+ars+raaGGr+g+++hnayGyGlftGGlGvh lcl|NCBI__GCF_000015165.1:WP_012043018.1 89 FAVPREQLVRVHASSGTTGKPIVVGYTRNDIATWSHVMARSIRAAGGRSGMIIHNAYGYGLFTGGLGVH 157 ********************************************************************* PP TIGR02155 139 yGaeklGatvvpisGGqtekqvqliqdfkpdiiavtpsyilalleelkrlgidpedislkvailGaepw 207 yGae lG+tvvpisGG+te+qvqli+df+pdii+vtpsy+la+l+e+kr+g+dp++ slk++i+Gaepw lcl|NCBI__GCF_000015165.1:WP_012043018.1 158 YGAEELGCTVVPISGGMTERQVQLINDFRPDIITVTPSYMLAILDEFKRQGLDPRQSSLKIGIFGAEPW 226 ********************************************************************* PP TIGR02155 208 teamrkelearlgikaldiyGlseviGpGvanecvetkdGlviwedhfypeiidpetgevlpdGeeGel 276 t+amr+e+e+++ ++a+diyGlseviGpGva+ec+etkdGl+iwedhfypei+dp+tg+vlpdGe Gel lcl|NCBI__GCF_000015165.1:WP_012043018.1 227 TNAMRAEIEDAFAMDATDIYGLSEVIGPGVAQECLETKDGLHIWEDHFYPEIVDPDTGKVLPDGEMGEL 295 ********************************************************************* PP TIGR02155 277 vfttltkealpviryrtrdltrllpgtart.mrrmdkikGrsddllilrGvnvfptqleevllkldkls 344 vft+ltkea+pviryrtrdltrllpgtar+ mrrm+k++Grsdd++ilrGvn+fp+q+ee+ll++++++ lcl|NCBI__GCF_000015165.1:WP_012043018.1 296 VFTSLTKEAFPVIRYRTRDLTRLLPGTARPgMRRMEKVTGRSDDMIILRGVNLFPSQIEEILLTTEWCG 364 *****************************99************************************** PP TIGR02155 345 phyqleltreGaldeltlkvelkdesaalrlleqksllakkiekkikaevgvsvdvelvepgslerseG 413 +h++leltreG++delt+ +e ++e ++ + l + a++i+ +ik+++gv+++v+ v p +lers G lcl|NCBI__GCF_000015165.1:WP_012043018.1 365 GHFMLELTREGRMDELTIYAESRPEHWDETGLGAH---AERISIHIKNTIGVTAKVKAVPPDTLERSAG 430 ****************************9988777...9****************************** PP TIGR02155 414 kakrvvdkr 422 kakr+ d+r lcl|NCBI__GCF_000015165.1:WP_012043018.1 431 KAKRIKDRR 439 *******98 PP Internal pipeline statistics summary: ------------------------------------- Query model(s): 1 (422 nodes) Target sequences: 1 (442 residues searched) Passed MSV filter: 1 (1); expected 0.0 (0.02) Passed bias filter: 1 (1); expected 0.0 (0.02) Passed Vit filter: 1 (1); expected 0.0 (0.001) Passed Fwd filter: 1 (1); expected 0.0 (1e-05) Initial search space (Z): 1 [actual number of targets] Domain search space (domZ): 1 [number of targets reported over threshold] # CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01 # Mc/sec: 10.59 // [ok]
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory