GapMind for catabolism of small carbon sources

 

Protein WP_012102195.1 in Clostridium kluyveri DSM 555

Annotation: NCBI__GCF_000016505.1:WP_012102195.1

Length: 352 amino acids

Source: GCF_000016505.1 in NCBI

Candidate for 62 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
putrescine catabolism potA med Spermidine/putrescine import ATP-binding protein PotA, component of The spermidine/putrescine uptake porter, PotABCD (characterized) 43% 85% 241.9 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism malK_Bb med ABC-type maltose transport, ATP binding protein (characterized, see rationale) 44% 71% 218.4 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 40% 86% 217.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 40% 86% 217.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism thuK med ThuK aka RB0314 aka SMB20328, component of Trehalose/maltose/sucrose porter (trehalose inducible) (characterized) 40% 86% 217.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
xylitol catabolism Dshi_0546 med ABC transporter for Xylitol, ATPase component (characterized) 40% 80% 210.3 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-fucose catabolism SM_b21106 med ABC transporter for L-Fucose, ATPase component (characterized) 42% 74% 207.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism malK1 lo MalK; aka Sugar ABC transporter, ATP-binding protein, component of The maltose, maltotriose, mannotetraose (MalE1)/maltose, maltotriose, trehalose (MalE2) porter (Nanavati et al., 2005). For MalG1 (823aas) and MalG2 (833aas), the C-terminal transmembrane domain with 6 putative TMSs is preceded by a single N-terminal TMS and a large (600 residue) hydrophilic region showing sequence similarity to MLP1 and 2 (9.A.14; e-12 & e-7) as well as other proteins (characterized) 49% 63% 225.7 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-mannitol catabolism mtlK lo SmoK aka POLK, component of Hexitol (glucitol; mannitol) porter (characterized) 38% 90% 214.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
N-acetyl-D-glucosamine catabolism SMc02869 lo N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 37% 91% 213.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucosamine (chitosamine) catabolism SMc02869 lo N-Acetyl-D-glucosamine ABC transport system, ATPase component (characterized) 37% 91% 213.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-cellobiose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-galactose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
lactose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-mannose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism glcV lo monosaccharide-transporting ATPase (EC 3.6.3.17) (characterized) 36% 99% 211.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism malK_Sm lo MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) 37% 94% 209.9 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism malK lo MalK, component of Maltose/Maltotriose/maltodextrin (up to 7 glucose units) transporters MalXFGK (MsmK (3.A.1.1.28) can probably substitute for MalK; Webb et al., 2008) (characterized) 37% 94% 209.9 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism treV lo TreV, component of Trehalose porter (characterized) 37% 99% 209.5 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-sorbitol (glucitol) catabolism mtlK lo ABC transporter for D-Sorbitol, ATPase component (characterized) 43% 67% 209.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
lactose catabolism lacK lo ABC transporter for Lactose, ATPase component (characterized) 35% 94% 208.4 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism malK lo Maltose/maltodextrin import ATP-binding protein MalK; EC 7.5.2.1 (characterized) 42% 63% 208.4 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism aglK lo ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) 42% 65% 206.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism aglK lo ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) 42% 65% 206.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism aglK lo ABC transporter for D-Maltose and D-Trehalose, ATPase component (characterized) 42% 65% 206.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-cellobiose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
lactose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-mannose catabolism TT_C0211 lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism gtsD lo Sugar-binding transport ATP-binding protein aka MalK1 aka TT_C0211, component of The trehalose/maltose/sucrose/palatinose porter (TTC1627-9) plus MalK1 (ABC protein, shared with 3.A.1.1.24) (Silva et al. 2005; Chevance et al., 2006). The receptor (TTC1627) binds disaccharide alpha-glycosides, namely trehalose (alpha-1,1), sucrose (alpha-1,2), maltose (alpha-1,4), palatinose (alpha-1,6) and glucose (characterized) 43% 63% 206.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-galactose catabolism PfGW456L13_1897 lo ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) 45% 61% 204.9 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-arabinose catabolism xacJ lo Xylose/arabinose import ATP-binding protein XacJ; EC 7.5.2.13 (characterized, see rationale) 45% 62% 203.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-xylose catabolism gtsD lo ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) 45% 61% 203.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-arabinose catabolism araV lo AraV, component of Arabinose, fructose, xylose porter (characterized) 37% 98% 202.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-fructose catabolism araV lo AraV, component of Arabinose, fructose, xylose porter (characterized) 37% 98% 202.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism araV lo AraV, component of Arabinose, fructose, xylose porter (characterized) 37% 98% 202.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-xylose catabolism araV lo AraV, component of Arabinose, fructose, xylose porter (characterized) 37% 98% 202.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-cellobiose catabolism SMc04256 lo ABC transporter for D-Cellobiose and D-Salicin, ATPase component (characterized) 35% 99% 199.1 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism malK_Aa lo ABC-type maltose transporter (EC 7.5.2.1) (characterized) 39% 74% 195.7 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucosamine (chitosamine) catabolism SM_b21216 lo ABC transporter for D-Glucosamine, ATPase component (characterized) 44% 65% 194.5 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-arabinose catabolism xacK lo Xylose/arabinose import ATP-binding protein XacK; EC 7.5.2.13 (characterized, see rationale) 39% 73% 193.4 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-cellobiose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
lactose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
sucrose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
trehalose catabolism aglK' lo Maltose/maltodextrin import ATP-binding protein; EC 3.6.3.19 (characterized, see rationale) 34% 91% 191.8 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-maltose catabolism musK lo ABC-type maltose transporter (EC 7.5.2.1) (characterized) 39% 74% 190.3 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
xylitol catabolism HSERO_RS17020 lo ABC-type sugar transport system, ATPase component protein (characterized, see rationale) 46% 53% 190.3 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-cellobiose catabolism msiK lo MsiK protein, component of The cellobiose/cellotriose (and possibly higher cellooligosaccharides), CebEFGMsiK [MsiK functions to energize several ABC transporters including those for maltose/maltotriose and trehalose] (characterized) 35% 86% 178.7 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
D-glucosamine (chitosamine) catabolism AO353_21725 lo ABC transporter for D-glucosamine, ATPase component (characterized) 38% 92% 157.5 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
glycerol catabolism glpT lo GlpT, component of Glycerol uptake porter, GlpSTPQV (characterized) 33% 85% 156 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
glycerol catabolism glpS lo GlpS, component of Glycerol uptake porter, GlpSTPQV (characterized) 36% 62% 141 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-isoleucine catabolism livG lo ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 31% 98% 135.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-leucine catabolism livG lo ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 31% 98% 135.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-valine catabolism livG lo ABC transporter ATP-binding protein-branched chain amino acid transport, component of The branched chain hydrophobic amino acid transporter, LivJFGHM (characterized) 31% 98% 135.6 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3
L-arabinose catabolism xylGsa lo Xylose/arabinose import ATP-binding protein XylG; EC 7.5.2.13 (characterized, see rationale) 33% 86% 120.2 CysA aka B2422, component of Sulfate/thiosulfate porter 43% 262.3

Sequence Analysis Tools

View WP_012102195.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MYVEVRNLNKNFGSYEASKNISFGIERGKLIGLLGPSGSGKTTILRIIAGLETADSGEIY
IDGKLVNDISPGKRGIGFVFQNYALFRNMTVYQNIAFGLSIKKEDKKYIKERVTELIELI
GLKGLEKRYPSQLSGGQKQRVAFARAIAPKPQLLLLDEPFAAIDAKVRKELRTWLKQMIH
KLGITSVFVTHDQEEAVEVADEIIITNLGSIEQIGSAVEIYKNPQTAFVATFVGESTIIK
DFSEFKAFKRLPSGAKAIIRPEFVEIGRKGEIQSELACDKGTITDISFAGSNWVVTVKIG
NTEISGVYSLEKDNLNLGEEVTVLVHRLYLFNDNKVEIIENNLKSDITSAYI

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory