Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_012112925.1 XAUT_RS04535 3-hydroxyacyl-CoA dehydrogenase
Query= reanno::BFirm:BPHYT_RS13545 (706 letters) >NCBI__GCF_000017645.1:WP_012112925.1 Length = 704 Score = 610 bits (1573), Expect = e-179 Identities = 338/686 (49%), Positives = 442/686 (64%), Gaps = 10/686 (1%) Query: 15 RGKVLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAGADIREFG 74 +GKV ++ ID APVNALS VR+GLL A+ +AD V A++I G FIAGADIRE Sbjct: 17 QGKVGVIAIDFAPVNALSKAVRQGLLDAVRTLEADPDVAALVISGGPGRFIAGADIREMS 76 Query: 75 KPPVPPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLGLPEVQLG 134 +PP P LPDV ++AC KPVVAAI GAALGGG E+ALA R+ A +GL E +LG Sbjct: 77 QPPDAPFLPDVVAAVDACAKPVVAAIAGAALGGGCEIALACDLRLGGPKALVGLTETRLG 136 Query: 135 LLPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRLGSSDDILAEGLAYVHE 194 L+PGAGGTQR P L+G A+ LI GR A +ALA GL+D + D +LA +A Sbjct: 137 LIPGAGGTQRLPCLVGVAQAIALICEGRVLKAGDALALGLLDEVVEGD-LLATAIA---- 191 Query: 195 LLAAHAPVRRTRDAAALSDRAASLAAVATARAETAKKSRGLFSPLKIVDAVEAAIEQPFD 254 AP R +AL A AA A A K ++G+ + + + + AA E F Sbjct: 192 ----RAPGMPKRRLSALPVPARDAAAEEAAAARARKAAKGVPAIDEAIRVIRAAGEGDFA 247 Query: 255 EGLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAAKPRTLNTIGVVGGGTMGAG 314 GL +ER FL +S + L H FFAERE K P PR + + V+G GTMGAG Sbjct: 248 SGLALERAAFLALRESAEAKALRHLFFAEREAGKVPGLEGVAPRPVEAVAVIGAGTMGAG 307 Query: 315 IAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALMSRWSGSTSY 374 IA+A+ DAGL VT+IERD + A G + +Y + GRL+ + A +R + + + Sbjct: 308 IAMALADAGLAVTLIERDAEAAAAGFQRVSDLYARQVKSGRLTDAQGAERRARVTATADW 367 Query: 375 DALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALASSVSRPADVI 434 L+ DLV+EA FED+AVK +F LD V K GAVLATNTSYLD+DA+A++ SRP DV+ Sbjct: 368 GRLSAVDLVVEAAFEDMAVKADIFRRLDAVAKPGAVLATNTSYLDLDAIAAATSRPQDVV 427 Query: 435 GLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGNRVLAVYR 494 GLHFF+PAN+MKLLEVV P + + DV+ATA LAK++ K PV AG CDGFIGNR+ A+YR Sbjct: 428 GLHFFAPANVMKLLEVVRPARSAPDVIATALALAKRMGKQPVVAGNCDGFIGNRIYALYR 487 Query: 495 SAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAATRNPAARYVQ 554 A+ ++EDG SP ++DAA+ A+GF MG F V D++G DI +A RKRRAATR+PA RYV Sbjct: 488 RHAEVLVEDGTSPQEVDAALEAYGFAMGLFAVSDMSGLDIAYAMRKRRAATRDPAERYVA 547 Query: 555 IADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPRSFTDEEIIR 614 IADRL E+G G+K+G G+Y Y + + + T DP VEA+I RA GITPR+F+ +I R Sbjct: 548 IADRLVEQGRLGRKTGAGWYAY-DAAGAKTCDPVVEAVIAEARAEKGITPRAFSAGQIQR 606 Query: 615 RYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYADMVGLPKILADIRE 674 R +A M NEGA + E IALR D+D+ F++GYGFPR +GGPM AD +GL +L ++ Sbjct: 607 RLLAVMANEGAKALAEGIALRASDIDLAFVHGYGFPRTKGGPMWAADQMGLRAVLTEVEA 666 Query: 675 FAKEDPLFWKPSPLLIELVERGADFA 700 +P+PLL+ L G FA Sbjct: 667 AHAAGGAGSEPAPLLVALARDGHSFA 692 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1148 Number of extensions: 52 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 704 Length adjustment: 39 Effective length of query: 667 Effective length of database: 665 Effective search space: 443555 Effective search space used: 443555 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory