Alignments for a candidate for fadA in Azorhizobium caulinodans ORS 571

GapMind for catabolism of small carbon sources

Alignments for a candidate for fadA in Azorhizobium caulinodans ORS 571

Align acetyl-CoA acetyltransferase (EC 2.3.1.16) (characterized)
to candidate WP_012168837.1 AZC_RS01555 acetyl-CoA C-acetyltransferase

Query= metacyc::MONOMER-13585
         (391 letters)



>NCBI__GCF_000010525.1:WP_012168837.1
          Length = 392

 Score =  568 bits (1463), Expect = e-166
 Identities = 283/389 (72%), Positives = 329/389 (84%)

Query: 3   NVVIVSAARTAVGSFNGAFASTPAHDLGAAVIEAVVARAGIDKADVSETILGQVLTAGQG 62
           +VVIVSAART VGSFNGA ++ PAH LGA  I+A + RAG+  ++VSE ILGQVLTA QG
Sbjct: 4   DVVIVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTAAQG 63

Query: 63  QNPARQAHIKAGLPQESAAWSINQVCGSGLRAVALAAQHVQLGDASIVVAGGQENMSLSP 122
           QNPARQA I AG+P ES AW +NQVCGSGLR+VAL  Q ++ GD+S+VVAGGQE+MS S 
Sbjct: 64  QNPARQASIAAGVPIESPAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMSQST 123

Query: 123 HVAHLRAGQKMGDLSFIDSMIKDGLWDAFNGYHMGQTAENVAAKWQISRDMQDEFAVASQ 182
           H AHLR G +MG L  +D+MIKDGLWDAFNGYHMG TAENVA ++QI+R  QDEFAVASQ
Sbjct: 124 HAAHLRNGTRMGSLDLVDTMIKDGLWDAFNGYHMGNTAENVATQFQITRAEQDEFAVASQ 183

Query: 183 NKAEAAQKAGRFADEIVPFVIKTRKGDVTVDADEYIRHGATLDAMAKLRPAFIKDGTVTA 242
           +KAEAAQKAGRF +EIVP  I +RKGDV VD DEY RHGAT++AM KL+PAF+KDGTVTA
Sbjct: 184 HKAEAAQKAGRFKEEIVPVTISSRKGDVVVDTDEYPRHGATIEAMQKLKPAFVKDGTVTA 243

Query: 243 ANASGINDGAAAVLVMSAEEAEKRGLSPLARIASYATAGLDPSIMGVGPIHASRKALEKA 302
           ANASGINDG AAV++MSAE A K G +PLARI S+A AG+DP++MG GPI AS+ ALEKA
Sbjct: 244 ANASGINDGGAAVVLMSAERAAKEGKTPLARIVSWAQAGVDPAVMGTGPIPASKLALEKA 303

Query: 303 GWKVGDLDLVEANEAFAAQACAVNKDMGWDPSIVNVNGGAIAIGHPIGASGARVLNTLLF 362
           GW V DLDL+EANEAFAAQA AVNK +GWD + VNVNGGAIAIGHPIGASG R+L TLL 
Sbjct: 304 GWTVDDLDLIEANEAFAAQAIAVNKGLGWDTAKVNVNGGAIAIGHPIGASGTRILVTLLH 363

Query: 363 EMQRRNAKKGLATLCIGGGMGVAMCLERP 391
           EMQ+R+AKKGLATLCIGGGMG+A+C+ERP
Sbjct: 364 EMQKRDAKKGLATLCIGGGMGIALCVERP 392


Lambda     K      H
   0.317    0.130    0.375 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 501
Number of extensions: 15
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 391
Length of database: 392
Length adjustment: 31
Effective length of query: 360
Effective length of database: 361
Effective search space:   129960
Effective search space used:   129960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 50 (23.9 bits)

Align candidate WP_012168837.1 AZC_RS01555 (acetyl-CoA C-acetyltransferase)
to HMM TIGR01930 (acetyl-CoA C-acyltransferase (EC 2.3.1.16))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01930.hmm
# target sequence database:        /tmp/gapView.7511.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01930  [M=385]
Accession:   TIGR01930
Description: AcCoA-C-Actrans: acetyl-CoA C-acyltransferase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   8.7e-146  471.7  12.3   9.8e-146  471.5  12.3    1.0  1  lcl|NCBI__GCF_000010525.1:WP_012168837.1  AZC_RS01555 acetyl-CoA C-acetylt


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000010525.1:WP_012168837.1  AZC_RS01555 acetyl-CoA C-acetyltransferase
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  471.5  12.3  9.8e-146  9.8e-146       1     385 []       7     390 ..       7     390 .. 0.98

  Alignments for each domain:
  == domain 1  score: 471.5 bits;  conditional E-value: 9.8e-146
                                 TIGR01930   1 ivdavRtpigklggslkelsaedLlaavikelleragldpekidevilGnvlqageqaniaReaalaag 69 
                                               iv+a+Rtp+g+++g+l++l+a++L+a +ik++lerag+ ++++ evilG+vl+a++++n+aR+a +aag
  lcl|NCBI__GCF_000010525.1:WP_012168837.1   7 IVSAARTPVGSFNGALSTLPAHQLGAIAIKAALERAGVAASEVSEVILGQVLTAAQGQNPARQASIAAG 75 
                                               8******************************************************************** PP

                                 TIGR01930  70 lpesvpaltvnrvCaSglqAvalaaqkikaGeadvvvaGGvEsmSrvpillkaslrreslklgkakled 138
                                               +p + pa++vn+vC+Sgl++val++q+ik G+ ++vvaGG EsmS++ +  +    r++ ++g+ +l d
  lcl|NCBI__GCF_000010525.1:WP_012168837.1  76 VPIESPAWQVNQVCGSGLRSVALGYQAIKNGDSSLVVAGGQESMSQSTHAAHL---RNGTRMGSLDLVD 141
                                               ***********************************************865554...79*********** PP

                                 TIGR01930 139 qllkdl...vktklsmgetAenlakkygisReeqDeyalrShqkaakAieegkfkdeivpvevkgk..k 202
                                               +++kd+   + ++++mg+tAen+a++++i+R eqDe+a++S++ka++A+++g+fk+eivpv++  +  +
  lcl|NCBI__GCF_000010525.1:WP_012168837.1 142 TMIKDGlwdAFNGYHMGNTAENVATQFQITRAEQDEFAVASQHKAEAAQKAGRFKEEIVPVTISSRkgD 210
                                               *********99****************************************************999999 PP

                                 TIGR01930 203 kvvskDegirpnttlekLakLkpafkekkgstvtAgNssqlnDGAaalllmseevakelgltplarivs 271
                                               +vv++De +r + t+e+++kLkpaf  k+g tvtA+N+s++nDG aa++lms e+a++ g tplarivs
  lcl|NCBI__GCF_000010525.1:WP_012168837.1 211 VVVDTDEYPRHGATIEAMQKLKPAFV-KDG-TVTAANASGINDGGAAVVLMSAERAAKEGKTPLARIVS 277
                                               *************************9.59*.6************************************* PP

                                 TIGR01930 272 aavagvdpeemglgpvpAiekaLkkaglsisdidlvEinEAFAaqvlavekelgsldlekvNvnGGAiA 340
                                               +a+agvdp++mg+gp+pA++ aL+kag++++d+dl+E nEAFAaq++av+k lg  d++kvNvnGGAiA
  lcl|NCBI__GCF_000010525.1:WP_012168837.1 278 WAQAGVDPAVMGTGPIPASKLALEKAGWTVDDLDLIEANEAFAAQAIAVNKGLG-WDTAKVNVNGGAIA 345
                                               ******************************************************.88************ PP

                                 TIGR01930 341 lGHPlGasGarivltllkeLkergkkyGlatlCvggGqGaAvile 385
                                               +GHP+GasG+ri++tll+e+++r++k GlatlC+ggG+G+A+ +e
  lcl|NCBI__GCF_000010525.1:WP_012168837.1 346 IGHPIGASGTRILVTLLHEMQKRDAKKGLATLCIGGGMGIALCVE 390
                                               *****************************************9886 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (385 nodes)
Target sequences:                          1  (392 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.01s 00:00:00.02 Elapsed: 00:00:00.01
# Mc/sec: 8.30
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources