Alignments for a candidate for lacA' in Azorhizobium caulinodans ORS 571

GapMind for catabolism of small carbon sources

Alignments for a candidate for lacA' in Azorhizobium caulinodans ORS 571

Align periplasmic glucoside 3-dehydrogenase (lacA subunit) (EC 1.1.99.13) (characterized)
to candidate WP_012169494.1 AZC_RS04935 GMC family oxidoreductase

Query= reanno::Pedo557:CA265_RS15345
         (567 letters)



>NCBI__GCF_000010525.1:WP_012169494.1
          Length = 522

 Score =  207 bits (526), Expect = 1e-57
 Identities = 165/572 (28%), Positives = 257/572 (44%), Gaps = 83/572 (14%)

Query: 17  IVIGSGISGGWAAKELTEKGLRVLMLERGMNIEHITGYETAMKNPWDFKHAGKLTEEQKR 76
           +++GSG  GG    EL +KG++V++LE G   E     +  + + W+       T+ +  
Sbjct: 13  VIVGSGAGGGTLGNELAQKGIKVVILEAGARNE----IQDFVNDEWESFAQLAWTDMRVA 68

Query: 77  THPVQ-KRDYPYQEANEKWWVNDLECPYTEDKRFDWYRGFHVGGKSLMWGRQSYRLSDHN 135
           +   +  RD+P   A   W V                    VGG ++ W   S R   H 
Sbjct: 69  SGSWRVARDFPNLPA---WIVKS------------------VGGSTVHWAGASLRFEQHE 107

Query: 136 FEDNARDGHGS-------DWPVRYAELSPWYDYAERFAGISGSKENWPTCPDGQFLPPMD 188
           F+  A   +G        DWP+  AEL P+Y  AE   G++ +            +P + 
Sbjct: 108 FK--AASAYGGIKGANLLDWPITKAELDPYYAKAEDKMGVTRTNN----------IPGLP 155

Query: 189 LNIVEKSVKARIEEHYKRERIMMIGRVANLTVPHKGRGNCQYRNLCSRGCPFGAYFSTQS 248
            N  EK ++A   +   ++     GR+A  + P  GRG+CQ    C +GC  GA +ST  
Sbjct: 156 GNNNEKVLEAGARKIGYKD--FSTGRMAINSQPRDGRGSCQQIGFCFQGCKSGAKWSTLY 213

Query: 249 STLPAAMATKRLTLRPYSIVNHIIYDKDTKKAKGVMVIDAETNKTMEFYAKIVFVNGSTL 308
           + +P   AT  L +RP   V  I +D + K    V+  D ET  T    A++V V G+++
Sbjct: 214 TEIPKGEATGNLEVRPNCHVAKIEHDANGK-VTAVVYFD-ETGATQRQKARVVAVAGNSI 271

Query: 309 GSTFVLLNSTSEAHPNGLGNGSGQLGHNLMDHHFRCGASGEAEGFDDKYTYGRRANGIYI 368
            S  +LLNS S   PNGL N SGQ+G N M H      +G      +K  +  R   +  
Sbjct: 272 ESPRLLLNSASSLFPNGLANSSGQVGRNYMRH-----VTGSVYAAFEKPVHMYRGTTM-- 324

Query: 369 PRYQNIGNDKRDYLRGFGYQGGASRANWQGDVAELSFGADLKQKMTTPGKWSM------- 421
                I  D+  +  G G+ GG        ++  LS G         PG W         
Sbjct: 325 ---AGIIRDEARHDPGRGFAGGY-------EMETLSLGLPFMAAFLNPGAWGRSFTSAME 374

Query: 422 ------GLGGFGEMLPYYENKVYIDKTKKDKWGQPVLAIDCEYKENEKKMRVDMMNDAAE 475
                 G+   GE +P  +N + +DK  KDK+G PV  +  +   N+  MR       A 
Sbjct: 375 NYPRMAGMWLVGEDMPQEKNAITLDKEAKDKFGLPVANVHYDDHSNDVAMRNHGYQQGAA 434

Query: 476 MLEKAGMKNIKTFDNGCYPGMAIHEMGTARMGNDPKTSVLNKWNQMHEVNNVFVTDGSCM 535
           + E  G   ++T+    YP  + H +GT RM   P+  V+NK+ Q H++ N+FV+DGS  
Sbjct: 435 LYEAVGA--VQTYPTTPYP--STHNLGTNRMSEKPRDGVVNKFGQSHDIKNLFVSDGSQF 490

Query: 536 PSIACQNPSLTFMALTARACDYAVKELKKKNI 567
            + A  NP+LT +AL  R  D+  + +++++I
Sbjct: 491 TTSAACNPTLTIVALAIRQADHMAELMRRRDI 522


Lambda     K      H
   0.317    0.134    0.421 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 771
Number of extensions: 46
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 567
Length of database: 522
Length adjustment: 35
Effective length of query: 532
Effective length of database: 487
Effective search space:   259084
Effective search space used:   259084
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources