Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_012172275.1 AZC_RS19330 dihydroxy-acid dehydratase
Query= reanno::BFirm:BPHYT_RS19730 (577 letters) >NCBI__GCF_000010525.1:WP_012172275.1 Length = 604 Score = 490 bits (1262), Expect = e-143 Identities = 272/594 (45%), Positives = 378/594 (63%), Gaps = 27/594 (4%) Query: 9 KPLRSQAWFGLKDRDGF--LHRSWMKNQGIPHDEFD-GRPVIGICNTWSELTPCNAHFRE 65 + LRSQ WF D G L+ N G+ +E G+P+IGI T S+L+PCN H + Sbjct: 13 RKLRSQLWFDNPDNPGMTALYLERYLNFGLTREELQSGKPIIGIAQTGSDLSPCNRHHLD 72 Query: 66 LAEYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNPMDGVILLV 125 LA+ V++G+ AGG+P EFPV + ET RPTA L RNLA + + E + G P+DGV+L Sbjct: 73 LAKRVREGIAAAGGIPFEFPVHPIQETGKRPTAALDRNLAYLGLVEVLYGYPLDGVVLTT 132 Query: 126 GCDKTTPALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVRAGTMTQEE 185 GCDKTTPA +M AA+ N+PA+ +SGGPMLNG + G+ GSGT VW+ + AG + + Sbjct: 133 GCDKTTPACIMAAATVNIPAIVLSGGPMLNGWWNGERTGSGTVVWKARAQHAAGEIDYDT 192 Query: 186 FTEAESCMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLAHLAGRRIV 245 F E + S GHC TMGTASTM + E+LGM LP AAIPA R +++ G+RIV Sbjct: 193 FMEIVASSAPSVGHCNTMGTASTMNGLAEALGMSLPGCAAIPAPYRERGQISYETGKRIV 252 Query: 246 DMVREDLTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLEDWE-LGSN 304 DMV EDL ILTR+AFENAIR N+AIGGSTNA +HL A+A+ +GV L+++DW+ +G Sbjct: 253 DMVWEDLKPSDILTREAFENAIRVNSAIGGSTNAPIHLNAIARHVGVSLTVDDWQAVGHK 312 Query: 305 VPCLVNLQPSGEYLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDNVRNAANYD 364 +P LVNLQP+GEYL E+F+ AGG+PAV+ +L + G +H+ ALTVNG+++ +N R ++D Sbjct: 313 IPLLVNLQPAGEYLGEEFHRAGGIPAVVAELIQAGQIHEGALTVNGRSIGENCRGKFSWD 372 Query: 365 EKVITTFAEPFKPKAGIAVLKGNLAPNGAVIKPSAATASLLKH-----------RGRAVV 413 VI + P K AG VL+GNL N A++K S + G AVV Sbjct: 373 RDVIRAYDAPMKADAGFIVLRGNLFEN-AIMKTSVISEEFRARFLSDPKDPNAFEGPAVV 431 Query: 414 FENIEELHAKIDDESLDIDEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQKGITDMVRI 473 FE E+ H +IDD +L IDEH ++ ++G GP GYPG AEV NM P ++++G+ + I Sbjct: 432 FEGPEDYHHRIDDPALGIDEHTLLFIRGTGPIGYPGGAEVVNMQPPAALIKRGVNALPCI 491 Query: 474 SDGRMSGTAYGAVVLHVSPEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDEELARRRAA 533 DGR SGT+ +L+ SPEAAAGG LA ++TGD + +D+E+ + ++DEELA+RRAA Sbjct: 492 GDGRQSGTSGSPSILNASPEAAAGGGLALLKTGDRVRIDLEKGTADILISDEELAQRRAA 551 Query: 534 WQA------PEAPKRGYYKLYVEHVLQADQGADLDFLVG----SSGAPVPRDSH 577 +A P++ + + ++ V Q D+G L V + VPRD+H Sbjct: 552 LEANGGFPIPQS-QTPWQEIQRSMVAQFDEGMVLKPAVKYQRVAQTMGVPRDNH 604 Lambda K H 0.318 0.135 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1013 Number of extensions: 62 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 577 Length of database: 604 Length adjustment: 37 Effective length of query: 540 Effective length of database: 567 Effective search space: 306180 Effective search space used: 306180 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (25.0 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory