GapMind for Amino acid biosynthesis

 

Alignments for a candidate for leuD in Methylobacterium sp. 4-46 Apr-46

Align 3-isopropylmalate dehydratase small subunit; Alpha-IPM isomerase; IPMI; Isopropylmalate isomerase; EC 4.2.1.33 (characterized)
to candidate WP_012330046.1 M446_RS00200 3-isopropylmalate dehydratase small subunit

Query= SwissProt::Q1MA52
         (202 letters)



>NCBI__GCF_000019365.1:WP_012330046.1
          Length = 200

 Score =  301 bits (772), Expect = 4e-87
 Identities = 146/201 (72%), Positives = 168/201 (83%), Gaps = 1/201 (0%)

Query: 1   MDKFVKLTGVAAPLPVVNVDTDMIIPKDYLKTIKRTGLGTGLFAEARYNEDGSENPDFVL 60
           M+KF  L GVAAPL +VNVDTD IIPK YLKTIKRTGLG GLF+E RY +DGSENPDFVL
Sbjct: 1   MEKFTVLEGVAAPLRIVNVDTDRIIPKQYLKTIKRTGLGKGLFSEMRYRDDGSENPDFVL 60

Query: 61  NKPAYRDAKILVAGDNFGCGSSREHAPWALLDFGIRCVISTSFADIFYNNCFKNGILPIK 120
           N+PAYR+AKILV GDNFGCGSSREHAPWAL DFGIRC++STSFADIF+NN  KNGIL I 
Sbjct: 61  NQPAYRNAKILVVGDNFGCGSSREHAPWALADFGIRCIVSTSFADIFFNNSAKNGILLIT 120

Query: 121 VSQEDLDKLMDDASRGSNAILTVDLENLEITGPDGGLIKFDLDEFKRHCLLNGLDDIGLT 180
           V+ EDL+KL DDA RG+NA LTVDLE  EI GPDGG++ FD+D  ++H LLNGLD+IGLT
Sbjct: 121 VAPEDLEKLFDDAERGANATLTVDLERQEIRGPDGGVLHFDIDPARKHTLLNGLDEIGLT 180

Query: 181 LEKGKAIDSFEKKNAASHPWA 201
           LEK  AI+++E+   A  PWA
Sbjct: 181 LEKAPAIEAYERA-LADRPWA 200


Lambda     K      H
   0.319    0.140    0.419 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 202
Number of extensions: 6
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 202
Length of database: 200
Length adjustment: 21
Effective length of query: 181
Effective length of database: 179
Effective search space:    32399
Effective search space used:    32399
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)

Align candidate WP_012330046.1 M446_RS00200 (3-isopropylmalate dehydratase small subunit)
to HMM TIGR00171 (leuD: 3-isopropylmalate dehydratase, small subunit (EC 4.2.1.33))

Software error:

../bin/blast/fastacmd -i /tmp/list.26680.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.26680.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

For help, please send mail to the webmaster (help@microbesonline.org), giving this error message and the time and date of the error.