Alignments for a candidate for trpA in Methylobacterium sp. 4-46 Apr-46

Align Tryptophan synthase alpha chain; EC 4.2.1.20 (characterized, see rationale)
to candidate WP_012330458.1 M446_RS02330 tryptophan synthase subunit alpha

Query= uniprot:M4NLA4
         (266 letters)



>NCBI__GCF_000019365.1:WP_012330458.1
          Length = 275

 Score =  226 bits (577), Expect = 3e-64
 Identities = 119/244 (48%), Positives = 158/244 (64%), Gaps = 1/244 (0%)

Query: 2   SRIDRRFAALKAANRTGLIPFVTAGDPSPEHMVALMHALVDAGADLIELGVPFSDPMADG 61
           +R+D  FA  +A  R  L+ +V AGDP PE  + ++ AL +AGAD++E G+PF+DPMADG
Sbjct: 3   TRLDDTFARCRAEGRAALVTYVMAGDPDPETSLQVLRALPEAGADIVEFGLPFTDPMADG 62

Query: 62  PVIQHASERAIAKGVGLADVLGWVAAFRQHDADTPIVLMGYLNPIETHGYARFAGEAVQA 121
           P IQ A  RA+  G  ++  L  V  FR  +  TP++LMGY NP+  +G ARF  +AV A
Sbjct: 63  PAIQAAGLRALKAGQTVSGTLALVRRFRAENRTTPVILMGYYNPLYIYGVARFLADAVAA 122

Query: 122 GVDGVLLVDCPLEESAVL-QPLRDAGLQRILLAAPTTEPSRMAQLCGSAEGFLYYVSFAG 180
           GVDG+++VD P EE   L  P ++AGL  I LA PTT+  R+  +  +  GF+YYVS  G
Sbjct: 123 GVDGLIVVDLPPEEDDELCLPTQEAGLAFIRLATPTTDERRLPAVLANTSGFVYYVSITG 182

Query: 181 ITGAAHLSTGDIAARVADVRARAKAPVAVGFGIRDAASAKAIAGFADAVVIGSALVDRLA 240
           ITG+A    G + A VA +RAR   PV VGFG++  A A AIA  AD VV+GSALVD LA
Sbjct: 183 ITGSATPDFGQVEAAVARIRARTALPVVVGFGVKTGAHAAAIARGADGVVVGSALVDALA 242

Query: 241 GATD 244
            + D
Sbjct: 243 RSLD 246


Lambda     K      H
   0.322    0.136    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 240
Number of extensions: 12
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 266
Length of database: 275
Length adjustment: 25
Effective length of query: 241
Effective length of database: 250
Effective search space:    60250
Effective search space used:    60250
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 47 (22.7 bits)

Align candidate WP_012330458.1 M446_RS02330 (tryptophan synthase subunit alpha)
to HMM TIGR00262 (trpA: tryptophan synthase, alpha subunit (EC 4.2.1.20))

Software error:

../bin/blast/fastacmd -i /tmp/list.30526.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.30526.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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