Alignments for a candidate for leuA in Methylobacterium sp. 4-46 Apr-46

Align 2-isopropylmalate synthase (EC 2.3.3.13) (characterized)
to candidate WP_012331377.1 M446_RS06860 2-isopropylmalate synthase

Query= BRENDA::Q9JZG1
         (517 letters)



>NCBI__GCF_000019365.1:WP_012331377.1
          Length = 519

 Score =  515 bits (1326), Expect = e-150
 Identities = 266/511 (52%), Positives = 358/511 (70%), Gaps = 2/511 (0%)

Query: 2   TQTNRVIIFDTTLRDGEQSPGAAMTKEEKIRVARQLEKLGVDIIEAGFAAASPGDFEAVN 61
           +   RV+IFDTTLRDGEQ PGA MT EEK+ VA  L+ +GVDIIEAGF  AS GDFEAV 
Sbjct: 6   SSAERVLIFDTTLRDGEQCPGATMTFEEKLAVAEMLDGMGVDIIEAGFPIASNGDFEAVT 65

Query: 62  AIAKTITKSTVCSLSRAIERDIRQAGEAVAPAPKKRIHTFIATSPIHMEYKLKMKPKQVI 121
            IA+   ++ V  L+RAI  DI +AGEAV PA + RIHTF++TSPIH+ ++++   ++V+
Sbjct: 66  EIARRTKRAVVAGLARAIPADIARAGEAVRPAARGRIHTFVSTSPIHLAHQMRKSEEEVL 125

Query: 122 EAAVKAVKIAREYTDDVEFSCEDALRSEIDFLAEICGAVIEAGATTINIPDTVGYSIPYK 181
           E   + V  AR+  +DVE+S  DA R+ +D+L       I  GATTIN+PDTVGY+ P +
Sbjct: 126 EIIQRTVAQARDLVEDVEWSAMDATRTPLDYLCRCVETAIRMGATTINLPDTVGYATPDE 185

Query: 182 TEEFFRELIAKTPNGGKVVWSAHCHNDLGLAVANSLAALKGGARQVECTVNGLGERAGNA 241
               FR +  + PN  K V+S HCHNDLGLA+ANSLA ++GGARQ+ECT+NG+GERAGNA
Sbjct: 186 YRAMFRAVRERVPNADKAVFSVHCHNDLGLAIANSLAGVEGGARQIECTINGIGERAGNA 245

Query: 242 SVEEIVMALKVRHDLFGLETGIDTTQIVPSSKLVSTITGYPVQPNKAIVGANAFSHESGI 301
           ++EE+VMA++ R D+    TGIDTTQI+ +SKLV+  T +PVQ NKAIVG NAF+HESGI
Sbjct: 246 ALEEVVMAIRTRGDVLPYATGIDTTQIMRASKLVAAATHFPVQYNKAIVGRNAFAHESGI 305

Query: 302 HQDGVLKHRETYEIMSAESVGWATNRLSLGKLSGRNAFKTKLADLGIELESEEALNAAFA 361
           HQDG+LK++ TYEIM+ ESVG     L +GK SGR AF++KL ++G  L S+     AF 
Sbjct: 306 HQDGMLKNQSTYEIMTPESVGVQKTSLVMGKHSGRAAFRSKLEEMGYRL-SDNQFQDAFE 364

Query: 362 RFKELADKKREIFDEDLHALVSDEMGSMNAESYKFISQKISTETGEEPRADIVFSIKGEE 421
           RFK LAD+K+ ++DED+ ALV + + + + +  + +S  +   T    RA +   I+G  
Sbjct: 365 RFKALADRKKNVYDEDIEALVDENLATAH-DRIRLVSLTVIAGTRGPQRATMRLDIEGRL 423

Query: 422 KRASATGSGPVDAIFKAIESVAQSGAALQIYSVNAVTQGTESQGETSVRLARGNRVVNGQ 481
               A G+GPVDA+F AI ++    A L++Y V+AVT+GT++Q E SVRL +G R V  +
Sbjct: 424 ATEEADGNGPVDAVFNAIHALVPHEAKLELYDVHAVTEGTDAQAEVSVRLRQGERSVTAR 483

Query: 482 GADTDVLVATAKAYLSALSKLEFSAAKPKAQ 512
           GAD D LVA+AKAYL+AL+KL   A +  AQ
Sbjct: 484 GADPDTLVASAKAYLAALNKLLAGANRLHAQ 514


Lambda     K      H
   0.313    0.129    0.352 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 617
Number of extensions: 19
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 517
Length of database: 519
Length adjustment: 35
Effective length of query: 482
Effective length of database: 484
Effective search space:   233288
Effective search space used:   233288
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 52 (24.6 bits)

Align candidate WP_012331377.1 M446_RS06860 (2-isopropylmalate synthase)
to HMM TIGR00973 (leuA: 2-isopropylmalate synthase (EC 2.3.3.13))

Software error:

../bin/blast/fastacmd -i /tmp/list.7842.in -d ../tmp/orgsDef_201/orgs.faa -p T > /tmp/gapView.7842.genome.faa failed: Inappropriate ioctl for device at ../lib/pbutils.pm line 379.

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