Alignments for a candidate for glpD in Beijerinckia indica ATCC 9039

GapMind for catabolism of small carbon sources

Alignments for a candidate for glpD in Beijerinckia indica ATCC 9039

Align glycerol-3-phosphate dehydrogenase (EC 1.1.5.3) (characterized)
to candidate WP_012383389.1 BIND_RS01920 FAD-dependent oxidoreductase

Query= BRENDA::Q06B39
         (650 letters)



>NCBI__GCF_000019845.1:WP_012383389.1
          Length = 539

 Score =  412 bits (1058), Expect = e-119
 Identities = 225/546 (41%), Positives = 321/546 (58%), Gaps = 29/546 (5%)

Query: 67  FDVLVIGGGATGAGCAFDAQTRGLQTALIEREDFASGTSSRSTKLVHGGVRYLEKAVFNL 126
           FD+LV+GGGATG G A DA +RGL+ AL+ER DFA GTSS+STKLVHGG RYLE A+  L
Sbjct: 16  FDLLVVGGGATGCGVALDAASRGLKVALVERNDFAEGTSSKSTKLVHGGARYLELAIRRL 75

Query: 127 DYDQLKLVYEALHERDRLLSNASHLAHPLPILTPCYNWWEVPYYWAGMKMYDLVAGTSTL 186
           D  Q  LV + L ER   L NA HLAH L +L+P Y W++VPY +AG+ +YD +AG   L
Sbjct: 76  DRVQFNLVRDGLRERAIFLKNAPHLAHRLALLSPLYRWFDVPYVFAGLYLYDRLAGKLGL 135

Query: 187 VPSKFLTSLETLTYLPTMSKNIGGKSLKGSIMYYDGQFNDSRMNVALACSSAAAGATVMN 246
             S+ L+  E L   P +      + LK +++YYDGQFND+RM V LA ++   GA +  
Sbjct: 136 GGSRLLSRAEALRRFPMLK----AEGLKAAVLYYDGQFNDARMAVTLALTAVEQGAVIAT 191

Query: 247 YTECKQLIKDDQGKVIGARCRDRATGAETDVYARVLINATGAFADDVRRYSEPDAQQTVM 306
             +   L  + +GK+ GA   DR +    ++ AR +INA G FAD +R  ++P+    + 
Sbjct: 192 RLDVVGLTHE-KGKLRGATVHDRESRKSFEIKARAIINAGGPFADSLRLMADPETAPILT 250

Query: 307 ASSGAHVTLPDFYGSARTGMIIPKTKDGRVIFMLPFQGHVIAGTTDTPIKATSRPRSSEE 366
           ASSG H+ L   +    TG++IP+T+DGR++F+LP+QGH + GTTD P +    P++ +E
Sbjct: 251 ASSGIHIVLDRRFAPTETGLMIPETEDGRLLFVLPWQGHALIGTTDEPARIEENPQARQE 310

Query: 367 DIDFILATLSDFLDVRVSRGDVLSTWCGIRPLPAPKKNTTSENVVRDHVIFEDKDGLLNV 426
            ID++L  +  + ++ V+  D+LS WCG+RPL +  K   +  + RDH+I   K GL+ +
Sbjct: 311 AIDYLLRHVRKYFNMNVTEADLLSVWCGLRPLVSDPKAADTARLARDHIIEVSKSGLVTI 370

Query: 427 TGGKWTTYRAMAEQAIDLAMASGKLPKSAKPCQTANYKLLGATNYTYTLMAELAQQHGMP 486
            GGKWTTYR MAEQ +D A+A   LP  +K C+T +  L+GA ++      + A      
Sbjct: 371 CGGKWTTYRKMAEQTVDHAIACFSLPTHSK-CRTLDLPLVGARHF------DAAGGAAAL 423

Query: 487 QRYFGKNSSKERNSNGSPRVAMDTEIAHHLSTAYGDNAMRVIAMAEEEGLGKRLIAGHPI 546
              FG                +  E A HL  AYGD A +V+   +   +   L   HP 
Sbjct: 424 TSTFG----------------LSPETAAHLHQAYGDRAKQVLGCVDTSAMKTLLHPLHPY 467

Query: 547 LEAEVMYTAKHEYCLTIEDFIDRRSRLAFLDVKATEAALPRIAEIMGNTLGWNDKQRTSA 606
           LEAEV+Y+A+ E  L+  D + RR  LA LD +A   A PR+  ++   L W D+QR + 
Sbjct: 468 LEAEVLYSARFEAALSAMDVLARRLPLALLDRQAAREAAPRVIAMLATELAW-DEQRCAN 526

Query: 607 ITAAVK 612
             A V+
Sbjct: 527 EAAEVE 532


Lambda     K      H
   0.317    0.132    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 806
Number of extensions: 32
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 1
Length of query: 650
Length of database: 539
Length adjustment: 37
Effective length of query: 613
Effective length of database: 502
Effective search space:   307726
Effective search space used:   307726
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources