Align 2-deoxy-D-ribonate 3-dehydrogenase (characterized)
to candidate WP_012385619.1 BIND_RS13595 3-hydroxybutyrate dehydrogenase
Query= reanno::Burk376:H281DRAFT_00644 (263 letters) >NCBI__GCF_000019845.1:WP_012385619.1 Length = 260 Score = 125 bits (313), Expect = 1e-33 Identities = 83/249 (33%), Positives = 129/249 (51%), Gaps = 9/249 (3%) Query: 17 VSAGAAGIGLAIAEAFIEAQAEVYICDVNQAAIDEATSRFPKLHAGI------ADVSKQA 70 V+ +GIGLA A AF + A V I AA E + G+ AD++K Sbjct: 9 VTGSTSGIGLAYARAFAKEGANVLINGFGDAAAIEKERAAIESEFGVKAIYSPADMTKAD 68 Query: 71 QVDQIIDDARRKLGGLDVLVNNAGIAGPTGAVEELDPAQWESTVSTNLNSQFYFLRKAVP 130 ++ ++I A + G +DVLVNNAGI +E+ +W ++ NL+S F+ + AVP Sbjct: 69 EIAEMIALAEKTFGSVDVLVNNAGIQF-VSPIEDFPIDKWNQIIAINLSSAFHTIHAAVP 127 Query: 131 VLKETSDCASIIAMSSVAGRLGYPFRTPYASTKWAIVGLVKSLAAELGPSNVRVNAILPG 190 +K II +S + PF++ Y + K I GL K++A E + NAI PG Sbjct: 128 GMKSRK-WGRIINTASAHALVASPFKSAYVAAKHGIAGLTKTVALETATFGITANAICPG 186 Query: 191 VVEGERMDRVISARADALGIPFNAMREEYLKKIS-LRRMVTVDDIAAMALFLASPAGSNV 249 V +++ I +A A GI A++ + L + VTV+ +AA+A+FLAS A +++ Sbjct: 187 YVWTPLVEKQIPDQAKARGISEEAVKRDVLLAAQPTKEFVTVEQVAALAVFLASDAAASI 246 Query: 250 TGQAISVDG 258 TG I +DG Sbjct: 247 TGSIIPIDG 255 Lambda K H 0.318 0.134 0.375 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 134 Number of extensions: 6 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 263 Length of database: 260 Length adjustment: 25 Effective length of query: 238 Effective length of database: 235 Effective search space: 55930 Effective search space used: 55930 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 47 (22.7 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory