Alignments for a candidate for HSERO_RS03640 in Beijerinckia indica ATCC 9039

GapMind for catabolism of small carbon sources

Alignments for a candidate for HSERO_RS03640 in Beijerinckia indica ATCC 9039

Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate WP_012385743.1 BIND_RS14235 sugar ABC transporter ATP-binding protein

Query= uniprot:D8IZC7
         (521 letters)



>NCBI__GCF_000019845.1:WP_012385743.1
          Length = 511

 Score =  398 bits (1023), Expect = e-115
 Identities = 208/495 (42%), Positives = 314/495 (63%), Gaps = 7/495 (1%)

Query: 6   LLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILL 65
           LL    IRK F   LAL  + L +R   +HALMGENGAGKSTLMK+++G++ PD G I L
Sbjct: 21  LLTAENIRKEFPGVLALDGVELKLRRSSVHALMGENGAGKSTLMKIIAGIYKPDAGRIRL 80

Query: 66  DGRPVALRDPGASRAAGINLIYQELAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTD 125
            G  ++ + P  +  +GI++I+QEL +   ++VA N+++  E   R G +DH  MR  T 
Sbjct: 81  KGNEISFQSPLDALESGISMIHQELNLMDYMTVAENIWIRREPLNRFGFVDHDEMRRMTQ 140

Query: 126 AVLRQLGAGFGASDLAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNV 185
           A+ R+L            LS+A +Q VEIA+A+ + S ++IMDEPT+AL+ERE + LF +
Sbjct: 141 ALFRRLRIDLDPDAEVRDLSVANRQIVEIAKAVSYGSDVLIMDEPTSALTEREVDHLFEI 200

Query: 186 VRRLRDEGLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEIDSERIVQMMVGRSLS 245
           +R LR +G+ I+YI+H+M E++ +AD  ++ RDG ++      ++  + I++MMVGR L+
Sbjct: 201 IRDLRAQGIGIVYITHKMNELFEIADEFSIFRDGRYISTHAACDVTCDDIIRMMVGRELT 260

Query: 246 EFYQHQRIAPADAAQLPTVMQVRALA-GGKIRPASFDVRAGEVLGFAGLVGAGRTELARL 304
           + +  + +   D A     + V+ +   G  +  +FD+RAGE+LG AGL+G+GR+ LA  
Sbjct: 261 QMFPKEEVPIGDVA-----LSVKNVGLDGVFQGVNFDLRAGEILGIAGLIGSGRSSLAAT 315

Query: 305 LFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPEDRKGQGLFLQMAVAANATMNV-A 363
           LFG  P + G I + G  V ID P  AMR  +A++ EDRK  G  L +++  N  + V  
Sbjct: 316 LFGVTPATSGSIAIAGNDVVIDSPATAMRHQMAFLTEDRKVSGCLLSLSILENLQLAVLQ 375

Query: 364 SRHTRLGLVRSRSLGGVARAAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVL 423
            ++   G V  + L        ++L VK  + +  +G LSGGNQQKVL+ RWL   P++L
Sbjct: 376 DKYVTNGFVHEKELFSACDEMARKLRVKTPNLQEIIGNLSGGNQQKVLIGRWLLNKPRIL 435

Query: 424 ILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSELPEVIGICDRVLVMREGMITGEL 483
           ILDEPTRG+D+ AK+EI++LV  LA +GVA+++ISSE+PEV+G+ DR++VM EG +TG L
Sbjct: 436 ILDEPTRGIDVGAKAEIHRLVTELAREGVAIILISSEMPEVLGMSDRIMVMHEGRMTGIL 495

Query: 484 AGAAITQENIMRLAT 498
             +  TQ  IM LA+
Sbjct: 496 DRSEATQVKIMELAS 510



 Score = 83.2 bits (204), Expect = 2e-20
 Identities = 57/226 (25%), Positives = 107/226 (47%), Gaps = 5/226 (2%)

Query: 282 VRAGEVLGFAGLVGAGRTELARLLFGADPRSGGDILLEGRPVHIDQPRAAMRAGIAYVPE 341
           +R   V    G  GAG++ L +++ G      G I L+G  +    P  A+ +GI+ + +
Sbjct: 44  LRRSSVHALMGENGAGKSTLMKIIAGIYKPDAGRIRLKGNEISFQSPLDALESGISMIHQ 103

Query: 342 DRKGQGLFLQMAVAANATMNVASRHTRLGLVRSRSLGGVARAAIQRLNVKVAHPETPVGK 401
           +     L   M VA N  +     + R G V    +  + +A  +RL + +  P+  V  
Sbjct: 104 ELN---LMDYMTVAENIWIRREPLN-RFGFVDHDEMRRMTQALFRRLRIDL-DPDAEVRD 158

Query: 402 LSGGNQQKVLLARWLEIAPKVLILDEPTRGVDIYAKSEIYQLVHRLASQGVAVVVISSEL 461
           LS  N+Q V +A+ +     VLI+DEPT  +       +++++  L +QG+ +V I+ ++
Sbjct: 159 LSVANRQIVEIAKAVSYGSDVLIMDEPTSALTEREVDHLFEIIRDLRAQGIGIVYITHKM 218

Query: 462 PEVIGICDRVLVMREGMITGELAGAAITQENIMRLATDTNVPRTAP 507
            E+  I D   + R+G      A   +T ++I+R+     + +  P
Sbjct: 219 NELFEIADEFSIFRDGRYISTHAACDVTCDDIIRMMVGRELTQMFP 264


Lambda     K      H
   0.320    0.135    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 669
Number of extensions: 28
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 521
Length of database: 511
Length adjustment: 35
Effective length of query: 486
Effective length of database: 476
Effective search space:   231336
Effective search space used:   231336
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources