Alignments for a candidate for xacD in Beijerinckia indica ATCC 9039

GapMind for catabolism of small carbon sources

Alignments for a candidate for xacD in Beijerinckia indica ATCC 9039

Align L-arabonate dehydratase (EC 4.2.1.25) (characterized)
to candidate WP_012385795.1 BIND_RS14500 dihydroxy-acid dehydratase

Query= reanno::BFirm:BPHYT_RS19730
         (577 letters)



>NCBI__GCF_000019845.1:WP_012385795.1
          Length = 599

 Score =  480 bits (1236), Expect = e-140
 Identities = 278/595 (46%), Positives = 370/595 (62%), Gaps = 25/595 (4%)

Query: 8   KKP--LRSQAWFGLKDRDGF--LHRSWMKNQGIPHDEFD-GRPVIGICNTWSELTPCNAH 62
           KKP  LRS+AWF          L+     N G+   E   G+P+IGI  T S+L+PCN  
Sbjct: 5   KKPEELRSRAWFDNPANPDMTALYLERYLNYGLTAKELQSGKPIIGIAQTGSDLSPCNRP 64

Query: 63  FRELAEYVKKGVHEAGGLPLEFPVMSLGETNLRPTAMLFRNLASMDVEESIRGNPMDGVI 122
             ELA+ V++G+ EAGG+ +EFPV  + ET  RPTA L RNLA + + E + G P+DGV+
Sbjct: 65  HLELAKRVREGIREAGGIAIEFPVHPIQETGKRPTAALDRNLAYLGLVEILYGYPLDGVV 124

Query: 123 LLVGCDKTTPALLMGAASCNLPALAVSGGPMLNGRFRGKNIGSGTGVWQMSEEVRAGTMT 182
           L  GCDKTTPA LM AA+ +LPA+ ++GGPMLNG F G+  GSGT +W+  E +  G + 
Sbjct: 125 LTTGCDKTTPACLMAAATVDLPAIVLTGGPMLNGWFNGERTGSGTIIWKARELLAKGEID 184

Query: 183 QEEFTEAESCMNRSRGHCMTMGTASTMASMVESLGMGLPHNAAIPAVDARRQVLAHLAGR 242
            ++F E  +    S G+C TMGTA+TM S+ E+LGM LP  AAIPA    R  +A+  G+
Sbjct: 185 YQKFIELVASSAPSAGYCNTMGTATTMNSLAEALGMSLPGCAAIPAPYRERGQIAYETGK 244

Query: 243 RIVDMVREDLTMDKILTRQAFENAIRTNAAIGGSTNAVVHLIALAKRIGVELSLEDWE-L 301
           RIVDMV EDL    I+TRQA EN I  N+AIGGSTNA +H+ A+A+ +GV L ++DWE +
Sbjct: 245 RIVDMVFEDLKPSDIITRQALENVIIVNSAIGGSTNAPIHINAIARHVGVPLEVDDWERV 304

Query: 302 GSNVPCLVNLQPSGEYLMEDFYYAGGLPAVLKQLGEQGLLHKEALTVNGKTLWDNVRNAA 361
           G  VP +VNLQP+G YL EDF+ AGG+PAV+ +L   GL+H+ ALTVNG+T+ DN    A
Sbjct: 305 GGQVPLIVNLQPAGTYLGEDFHRAGGVPAVVHELMRHGLIHENALTVNGRTMGDNCHERA 364

Query: 362 NYDEKVITTFAEPFKPKAGIAVLKGNLAPNG----AVIKPSAATASLLK------HRGRA 411
             D  VI TF     P AG  V++GNL  +      VI     T  L          GRA
Sbjct: 365 ALDADVIRTFETALVPAAGFKVVRGNLFDSAIMKTCVISDEFRTRYLSNPQDPDAFEGRA 424

Query: 412 VVFENIEELHAKIDDESLDIDEHCIMVLKGAGPKGYPGFAEVGNMPLPKKVLQKGITDMV 471
           VVF+  E+ H +IDD SL+IDEHC++ ++G G  G+PG AEV NM  P  +LQ+ IT + 
Sbjct: 425 VVFDGPEDYHQRIDDPSLNIDEHCLLFIRGTGAIGFPGSAEVVNMRAPSYLLQRNITSLP 484

Query: 472 RISDGRMSGTAYGAVVLHVSPEAAAGGPLAFVQTGDMIELDVEERRLHLDVTDEELARRR 531
            I DGR SGT+    +L+ SPEAAAGG LA ++TGD + +D+ + R  + ++DEELA+RR
Sbjct: 485 CIGDGRQSGTSGSPSILNASPEAAAGGGLALLRTGDRVRIDLLKGRADILISDEELAQRR 544

Query: 532 AAWQAPEAPK-----RGYYKLYVEHVLQADQGADLDFLVG----SSGAPVPRDSH 577
           A +QA    K       + ++    V Q  QG  L   V     +S   VPRDSH
Sbjct: 545 ADFQAHGGYKYPPSQTPWQEIQRGIVDQLAQGMVLKPAVKYQRVASTYGVPRDSH 599


Lambda     K      H
   0.318    0.135    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 981
Number of extensions: 56
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 577
Length of database: 599
Length adjustment: 37
Effective length of query: 540
Effective length of database: 562
Effective search space:   303480
Effective search space used:   303480
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources