Alignments for a candidate for frcA in Beijerinckia indica ATCC 9039

GapMind for catabolism of small carbon sources

Alignments for a candidate for frcA in Beijerinckia indica ATCC 9039

Align ABC-type sugar transport system, ATP-binding protein; EC 3.6.3.17 (characterized, see rationale)
to candidate WP_012385982.1 BIND_RS15565 sugar ABC transporter ATP-binding protein

Query= uniprot:A0A0C4Y5F6
         (540 letters)



>NCBI__GCF_000019845.1:WP_012385982.1
          Length = 511

 Score =  336 bits (862), Expect = 1e-96
 Identities = 204/510 (40%), Positives = 306/510 (60%), Gaps = 25/510 (4%)

Query: 13  LLALRNICKTFPGVRALRKVELTAYAGEVHALMGENGAGKSTLMKILSGAYT-ADPGGEC 71
           +L +R I K+F GV+AL  V LT  AGE+HAL+GENGAGKSTLMK+LSG Y      G  
Sbjct: 4   ILEMRGISKSFTGVKALNDVNLTVKAGEIHALVGENGAGKSTLMKVLSGVYPHGSYEGAI 63

Query: 72  HIDGQRVQIDGPQSARDLGVAVIYQELSLAPNLSVAENIYLGRALQRRGLVARGDMVRAC 131
             DG+            LG+ +I+QEL+L P +S+AENI++     R G++ R  +    
Sbjct: 64  FYDGEERHFHDINDTEALGIIIIHQELALIPLMSIAENIFIANPPSRFGVIDRDTVYTRA 123

Query: 132 APTLARLGADFSPAANVASLSIAQRQLVEIARAVHFEARILVMDEPTTPLSTHETDRLFA 191
              LA++G + +P   + ++ + ++QLVEIA+A+  + R+L++DEPT  L+ ++++ L  
Sbjct: 124 KELLAKVGLNEAPDTLITNIGVGKQQLVEIAKALSKKVRLLILDEPTASLNENDSEALLD 183

Query: 192 LIRQLRGEGMAILYISHRMAEIDELADRVTVLRDGCFVGTLD--RAHLSQAALVKMMVGR 249
           L+ + R +G+  + ISH++ E+  +AD++TVLRDG  V T+D     + +  +++ MV R
Sbjct: 184 LLIEFRAQGITSILISHKLNEVSRVADQITVLRDGRTVDTMDCHAEAIEEDRIIRKMVDR 243

Query: 250 DLSGFYTKTH---GQAVEREVMLSV--RDVADGRRVKGCSFDLRAGEVLGLAGLVGAGRT 304
           DL   Y K     G A+      SV      D + +K  +F ++ GE++G+AGL+GAGRT
Sbjct: 244 DLEHRYPKREPKIGAAIFSIENWSVYHPQHPDRQLIKNVNFHVKRGEIVGIAGLMGAGRT 303

Query: 305 ELARLVFGADARTRGEVRIANPAGSGGLVTLPAGGPRQAIDAGIAYLTEDRKLQGLFLDQ 364
           E A  VFG   R+ G+        +G  V L      +AI+AG+AY+TEDRK  GL L  
Sbjct: 304 EFAMSVFG---RSWGQKITGRAVLNGREVDLST--VSRAINAGLAYVTEDRKELGLLLSD 358

Query: 365 SVHENINLI----VAARDALGLGRLNRTAARRRTTEAIDTLGIRVAHAQVNVGALSGGNQ 420
            + +N+ L     VA +  +   +  + A+  R+   I + G+        VG LSGGNQ
Sbjct: 359 DIRKNVTLANLEGVANQRVIDDMKEFKVASDYRSRMRIRSSGV-----YQEVGKLSGGNQ 413

Query: 421 QKVMLSRLLEIQPRVLILDEPTRGVDIGAKSEIYRLINALAQSGVAILMISSELPEVVGL 480
           QKV+LS+ L   P +LILDEPTRG+DIGAK EIY +IN LA +G  +++ISSE+PE++G+
Sbjct: 414 QKVVLSKWLFAGPEILILDEPTRGIDIGAKYEIYCIINDLANAGKGVVVISSEMPELLGI 473

Query: 481 CDRVLVMREGTLAGEVRPAGSAAETQERII 510
            DR+ VM EG   GE     +A  TQE+I+
Sbjct: 474 SDRIYVMNEGAFVGEF---PTAEATQEKIM 500



 Score = 63.2 bits (152), Expect = 2e-14
 Identities = 49/231 (21%), Positives = 104/231 (45%), Gaps = 7/231 (3%)

Query: 24  PGVRALRKVELTAYAGEVHALMGENGAGKSTL-MKILSGAYTADPGGECHIDGQRVQIDG 82
           P  + ++ V      GE+  + G  GAG++   M +   ++     G   ++G+ V +  
Sbjct: 274 PDRQLIKNVNFHVKRGEIVGIAGLMGAGRTEFAMSVFGRSWGQKITGRAVLNGREVDLST 333

Query: 83  PQSARDLGVAVIYQ---ELSLAPNLSVAENIYLGR--ALQRRGLVARGDMVRACAPTLAR 137
              A + G+A + +   EL L  +  + +N+ L     +  + ++      +  +   +R
Sbjct: 334 VSRAINAGLAYVTEDRKELGLLLSDDIRKNVTLANLEGVANQRVIDDMKEFKVASDYRSR 393

Query: 138 LGADFSPA-ANVASLSIAQRQLVEIARAVHFEARILVMDEPTTPLSTHETDRLFALIRQL 196
           +    S     V  LS   +Q V +++ +     IL++DEPT  +       ++ +I  L
Sbjct: 394 MRIRSSGVYQEVGKLSGGNQQKVVLSKWLFAGPEILILDEPTRGIDIGAKYEIYCIINDL 453

Query: 197 RGEGMAILYISHRMAEIDELADRVTVLRDGCFVGTLDRAHLSQAALVKMMV 247
              G  ++ IS  M E+  ++DR+ V+ +G FVG    A  +Q  +++ ++
Sbjct: 454 ANAGKGVVVISSEMPELLGISDRIYVMNEGAFVGEFPTAEATQEKIMRSIM 504


Lambda     K      H
   0.320    0.136    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 577
Number of extensions: 26
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 3
Number of HSP's successfully gapped: 2
Length of query: 540
Length of database: 511
Length adjustment: 35
Effective length of query: 505
Effective length of database: 476
Effective search space:   240380
Effective search space used:   240380
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources