Align Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale)
to candidate WP_012386370.1 BIND_RS17610 ABC transporter ATP-binding protein
Query= uniprot:D8IZC7 (521 letters) >NCBI__GCF_000019845.1:WP_012386370.1 Length = 548 Score = 159 bits (403), Expect = 2e-43 Identities = 159/516 (30%), Positives = 248/516 (48%), Gaps = 53/516 (10%) Query: 2 TQTPLLQMRGIRKSFGATLALSDMHLTIRPGEIHALMGENGAGKS-TLMKVLSGVHAPDQ 60 T PLL R + S+G + D+ L++ PGE AL+GE+G+GKS T + L + P + Sbjct: 14 TDKPLLAFRDLSISYGGQAIVRDLTLSLHPGETAALVGESGSGKSQTALAALRLLPEPAK 73 Query: 61 --GEILLDGRPV-ALRDPGASRAAG--INLIYQE--LAVAPNISVAANVFMGSELRTRLG 113 G+IL +G+ + +L + +R G I LI+QE A+ P V A + G LR LG Sbjct: 74 VSGQILFEGQDLLSLSELALNRLRGRRIALIFQEPMSALDPLFPVGAQI--GVILRHHLG 131 Query: 114 LIDHAAMRSRTDAVLRQLGAGFGASDLAG---RLSIAEQQQVEIARALVHRSRIVIMDEP 170 L AA+ +R +L Q+G + LS ++Q+V IA A+ ++I DEP Sbjct: 132 LSRRAAL-TRACELLDQVGIPDPQRRVKAYPHELSGGQRQRVAIAMAIACEPAVLIADEP 190 Query: 171 TAALSERETEQLFNVVRRLRDE-GLAIIYISHRMAEVYALADRVTVLRDGSFVGELVRDE 229 T AL ++ +++ L+ G+A+++ISH + V +AD V V+ G V E Sbjct: 191 TTALDVTLAARILDLIEDLKQRLGMALLFISHDLGLVRRIADSVHVMHQGRIVESGPARE 250 Query: 230 I---DSERIVQMMVGRSLSEFYQHQRIAP--------ADAAQLPTVMQVRALAG----GK 274 I E ++++G + R+ P +A L + R G G Sbjct: 251 IIHHPREAYTRLLLGAA-----PRPRLRPHPLEAKNLLEARDLTVRFKRRGTLGLPSFGL 305 Query: 275 IRPASFD----------VRAGEVLGFAGLVGAGRTELARLLFGADPRSGGDILLEGRPVH 324 RP D +R GE G G G+G++ LAR L P SG IL EGR + Sbjct: 306 FRPREKDFTAVDHVNLTLRQGETFGLVGESGSGKSTLARALLRLVPASGS-ILFEGRDLV 364 Query: 325 IDQPRAA--MRAGIAYVPEDRKGQGLFLQMAVAANATMNVASRHTRLGLVRSRSLGGVAR 382 QPRA R + V +D Q L +M + A T + L +++L A Sbjct: 365 ALQPRALRPFRHAMQIVFQDPY-QALSPRMRIGAIITEGLRIHEPGLN---AQALDARAA 420 Query: 383 AAIQRLNVKVAHPETPVGKLSGGNQQKVLLARWLEIAPKVLILDEPTRGVDIYAKSEIYQ 442 A+Q + + A + SGG +Q++ +AR + + PK+L+LDEPT +D ++EI + Sbjct: 421 FALQEVKLDPALRHRFPAECSGGQRQRIAIARAMILRPKLLLLDEPTSALDRSVQAEILE 480 Query: 443 LVHRL-ASQGVAVVVISSELPEVIGICDRVLVMREG 477 L+ L AS G+ + IS + V + D + VMR G Sbjct: 481 LLSLLQASHGLTYLFISHDFGAVRAMADTIGVMRMG 516 Score = 89.7 bits (221), Expect = 2e-22 Identities = 64/216 (29%), Positives = 115/216 (53%), Gaps = 9/216 (4%) Query: 21 ALSDMHLTIRPGEIHALMGENGAGKSTLMKVLSGVHAPDQGEILLDGRPVALRDPGASRA 80 A+ ++LT+R GE L+GE+G+GKSTL + L + P G IL +GR + P A R Sbjct: 315 AVDHVNLTLRQGETFGLVGESGSGKSTLARALLRL-VPASGSILFEGRDLVALQPRALRP 373 Query: 81 A--GINLIYQE--LAVAPNISVAANVFMGSELRTRLGLIDHAAMRSRTDAVLRQLGAGFG 136 + +++Q+ A++P + + A + G LR ++ A+ +R L+++ Sbjct: 374 FRHAMQIVFQDPYQALSPRMRIGAIITEG--LRIHEPGLNAQALDARAAFALQEVKLDPA 431 Query: 137 ASD-LAGRLSIAEQQQVEIARALVHRSRIVIMDEPTAALSERETEQLFNVVRRLR-DEGL 194 S ++Q++ IARA++ R +++++DEPT+AL ++ ++ L+ GL Sbjct: 432 LRHRFPAECSGGQRQRIAIARAMILRPKLLLLDEPTSALDRSVQAEILELLSLLQASHGL 491 Query: 195 AIIYISHRMAEVYALADRVTVLRDGSFVGELVRDEI 230 ++ISH V A+AD + V+R G V +EI Sbjct: 492 TYLFISHDFGAVRAMADTIGVMRMGHLVESGPAEEI 527 Lambda K H 0.320 0.135 0.378 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 674 Number of extensions: 41 Number of successful extensions: 9 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 2 Number of HSP's successfully gapped: 2 Length of query: 521 Length of database: 548 Length adjustment: 35 Effective length of query: 486 Effective length of database: 513 Effective search space: 249318 Effective search space used: 249318 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 52 (24.6 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory