Align 3-hydroxyadipyl-CoA dehydrogenase (EC 1.1.1.35) (characterized)
to candidate WP_012400431.1 BPHY_RS05220 3-hydroxyacyl-CoA dehydrogenase
Query= reanno::BFirm:BPHYT_RS13545 (706 letters) >NCBI__GCF_000020045.1:WP_012400431.1 Length = 694 Score = 587 bits (1513), Expect = e-172 Identities = 311/688 (45%), Positives = 442/688 (64%), Gaps = 8/688 (1%) Query: 18 VLLVTIDHAPVNALSADVRRGLLAAIEAADADKAVEAVLIVGAGRNFIAGADIREFGKPP 77 + ++T+++ PVN L R G++ I+ A D AV+A+++ GAG+ F GADI EF P Sbjct: 11 IAVITLNNPPVNGLGLSTRMGIVEGIDRAQHDPAVKAIVLTGAGKAFSGGADITEFNTPK 70 Query: 78 V--PPSLPDVCNRIEACTKPVVAAIHGAALGGGLEVALAAHYRIAVDGAKLGLPEVQLGL 135 P+L V +E KPVVAAIH A+GGGLE+AL AHYR+A GA++ LPEV+LG+ Sbjct: 71 AFQEPTLATVIKAVEGSAKPVVAAIHSVAMGGGLELALGAHYRVAAPGAQIALPEVKLGI 130 Query: 136 LPGAGGTQRTPRLIGAQAALDLILSGRHASAKEALAFGLIDRLGSSDDILAEGLAYVHEL 195 LPGAGGTQR PR +G +AAL++I+SG +++ + D++ + +L + LA+ ++ Sbjct: 131 LPGAGGTQRLPRALGLEAALNMIVSGTPVMSEKLAGTPMFDQMVEGN-LLEDALAFARKV 189 Query: 196 LAAHAPVRRTRDAAALSDRAASLAAVATARAETAKKSRGLFSPLKIVDAVEAAIEQPFDE 255 A P + RD AA + AR A ++ +P K +DA+EA + + FD Sbjct: 190 GAQSGPHPKIRDRNIGHPNAAGF--IQFARNSVAGVAKQYPAPHKCIDAIEAGVLKGFDH 247 Query: 256 GLRVERKLFLECIDSPQRAGLIHAFFAEREVLKAPETRAAKP-RTLNTIGVVGGGTMGAG 314 G++VER+ FL + +P+ L HAFF ER K P+ + P R ++ +GV+G GTMG G Sbjct: 248 GVKVERECFLALVQTPESKALRHAFFGERAASKIPDVPSDTPARNIDKVGVIGAGTMGGG 307 Query: 315 IAVAVLDAGLPVTMIERDDASLARGRAHIEKVYDGLIAKGRLSAEKKAALMSRWSGSTSY 374 IA+ ++AGLPVT++E +L RG A I K Y+ + KG+L+ E MS + + +Y Sbjct: 308 IAMNFVNAGLPVTLLEMKQDALERGLATIRKNYEATVKKGKLTMEALEQRMSLITPTLTY 367 Query: 375 DALAGADLVIEAVFEDLAVKQAVFAELDRVCKAGAVLATNTSYLDIDALASSVSRPADVI 434 +AL ADL+IEAV+E+L VK+ VF LD V K GA+LA+NTS LD++ +A+ RP DV+ Sbjct: 368 EALGDADLIIEAVYEELGVKEQVFTRLDEVAKPGAILASNTSTLDLNKIAAFTKRPQDVV 427 Query: 435 GLHFFSPANIMKLLEVVVPKQVSADVVATAFELAKKLRKTPVRAGVCDGFIGNRVLAVYR 494 G+HFFSPAN+MKLLEVV K + DV+AT ++AKK+RKT V +GVCDGFIGNR++ Y Sbjct: 428 GMHFFSPANVMKLLEVVRGKDTAKDVLATVMQVAKKIRKTAVVSGVCDGFIGNRMVEQYV 487 Query: 495 SAADAMMEDGASPYQIDAAVRAFGFPMGPFQVVDLAGGDIGWAARKRRAATRNPAARYVQ 554 A M+E+GA P Q+D A+ FGF MGPF++ DLAG DIGWA RKRR P RY + Sbjct: 488 RQALFMLEEGALPAQVDKAIEKFGFAMGPFRMSDLAGNDIGWAIRKRR-YQEEPHMRYSK 546 Query: 555 IADRLCERGWFGQKSGRGFYLYPEGSRSGTPDPEVEAIIDAERARAGITPRSFTDEEIIR 614 IADRLCE G FGQK+G G+Y Y G R+ P ++ +I A GI R +D+EI+ Sbjct: 547 IADRLCEAGRFGQKTGGGWYDYKAGDRNAYPSKPIDEMIVAHSKELGIERRKISDDEIVE 606 Query: 615 RYMAAMINEGANVVHERIALRPLDVDVTFLYGYGFPRYRGGPMKYADMVGLPKILADIRE 674 R + A++NEGA ++ E IA + D+D+ +L GYGFP +RGGPM YAD VGL + IR Sbjct: 607 RLVFALVNEGAKILEEGIASKASDIDMVYLTGYGFPLWRGGPMLYADSVGLYNVERAIRR 666 Query: 675 FAKE-DPLFWKPSPLLIELVERGADFAS 701 +A + + W+ + ++E +G F S Sbjct: 667 YAAQPNGDAWQIAAGIVERAAQGRGFNS 694 Lambda K H 0.320 0.136 0.392 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1083 Number of extensions: 52 Number of successful extensions: 5 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 706 Length of database: 694 Length adjustment: 39 Effective length of query: 667 Effective length of database: 655 Effective search space: 436885 Effective search space used: 436885 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory