Align β-galactosidase (A4-β-Gal) (EC 3.2.1.23) (characterized)
to candidate WP_012401732.1 BPHY_RS11950 beta-galactosidase
Query= CAZy::BAA28362.1 (645 letters) >NCBI__GCF_000020045.1:WP_012401732.1 Length = 660 Score = 679 bits (1751), Expect = 0.0 Identities = 334/655 (50%), Positives = 430/655 (65%), Gaps = 14/655 (2%) Query: 2 LGVCYYPEHWPKERWKEDARRMREAGLSHVRIGEFAWALLEPEPGRLEWGWLDEAIATLA 61 LGVCYYPEHWP+ W++DARRM+ G+ VRI EFAW+ +EP PG +WGWLD A+ L Sbjct: 3 LGVCYYPEHWPESMWEDDARRMKALGIEQVRIAEFAWSRMEPTPGEYDWGWLDRAVDVLG 62 Query: 62 AEGLKVVLGTPTATPPKWLVDRYPEILPVDREGRRRRFGGRRHYCFSSPVYREEARRIVT 121 GL+VV+ TPTATPPKWL+DR+P+ILPV +GR R FG RRHY FSSP Y E +R+I T Sbjct: 63 KAGLQVVMCTPTATPPKWLIDRHPDILPVGADGRPRAFGSRRHYDFSSPSYFEASRKICT 122 Query: 122 LLAERYGGLEAVAGFQTDNEYGCHDTVRCYCPRCQEAFRGWLEARYGTIEALNEAWGTAF 181 +A RYG AVA +QTDNE+GCH+TV Y P FR WL+ARY TI+ALN AWGT F Sbjct: 123 AVAGRYGKHPAVAYWQTDNEFGCHNTVVSYSPAAVARFREWLKARYQTIDALNRAWGTVF 182 Query: 182 WSQRYRSFAEVELPHLTVAEPNPSHLLDYYRFASDQVRAFNRLQVEILRAHAPGKFVTHN 241 WS YRSF E++ P TV E +PSH LDY RFASD+V +NR+QVEI+RAH+PG+ V HN Sbjct: 183 WSMEYRSFDEIDAPIATVTEAHPSHRLDYRRFASDEVARYNRMQVEIIRAHSPGRPVAHN 242 Query: 242 FMGFFTDLDAFALAQDLDFASWDSYPLGFTDLMPLPPEEKLRYARTGHPDVAAFHHDLYR 301 FM FT+ D + +A+DLD A+WDSYPLG + PE K ++ RTGHPD A+F+HD+YR Sbjct: 243 FMQLFTEFDHYKVARDLDVATWDSYPLGALEEQWYAPEIKAKFLRTGHPDFASFNHDVYR 302 Query: 302 GVGRGRFWVMEQQPGPVNWAPHNPSPAPGMVRLWTWEALAHGAEVVSYFRWRQAPFAQEQ 361 G+ + FWVMEQQPGPVNWA NPSP PGMVRLW+WEA AHGA VSYFRWRQAPFAQEQ Sbjct: 303 GMSKLPFWVMEQQPGPVNWAHWNPSPLPGMVRLWSWEAFAHGAGCVSYFRWRQAPFAQEQ 362 Query: 362 MHAGLHRPDSAPDQGFFEAKRVAEELAALALPPV-----AQAPVALVFDYEAAWIYEVQP 416 MHAGL+ PD+ D G EA++VA E+A ++ ++ +AL++DYEA W++E+ P Sbjct: 363 MHAGLNTPDNRLDVGGSEAQKVAHEIAQVSSANADADANVRSKIALIYDYEAKWLFEIHP 422 Query: 417 QGAEWSYLGLVYLFYSALRRLGLDVDVVPPGASLRGYAFAVVPSLPIVREEALEAFREAE 476 QGA++ Y + +YSALR LG DVDV+ A GY VVP LP+V ++ + Sbjct: 423 QGADFHYPRFAFEYYSALRALGFDVDVIAADAPFDGYKMIVVPPLPVVPDDFAVRLAASG 482 Query: 477 GPVLFGPRSGSKTETFQIPKELPPGPLQALLPLKVVRVESLPPGLLEV-----AEGALGR 531 ++ GPR+GSKT QIP LPPG L +LLP++V RVES+ P + E A L Sbjct: 483 AQIVLGPRTGSKTADLQIPPNLPPGALASLLPIRVWRVESMRPNVTEPVHMNGAGDGLHE 542 Query: 532 FPLGLWREWVEAP----LKPLLTFQDGKGALYREGRYLYLAAWPSPELAGRLLSALAAEA 587 WR+ ++A F DG A + G Y A+ L L +A EA Sbjct: 543 GHARHWRDLIDAADERGFDVRARFDDGHPAYVQHGCVHYFASLFDDALTQALFGRIATEA 602 Query: 588 GLKVLSLPEGLRLRRRGTWVFAFNYGPEAVEAPASEGARFLLGSRRVGPYDLAVW 642 GL L + +R+ RRG + FNY + +RF++G+R V P ++ + Sbjct: 603 GLTPTLLGDSVRISRRGKLAYVFNYANAPHTIEGVDASRFVIGTREVEPQGVSAY 657 Lambda K H 0.322 0.139 0.454 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 1440 Number of extensions: 82 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 645 Length of database: 660 Length adjustment: 38 Effective length of query: 607 Effective length of database: 622 Effective search space: 377554 Effective search space used: 377554 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 54 (25.4 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory