Alignments for a candidate for aldA in Chlorobium limicola DSM 245

GapMind for catabolism of small carbon sources

Alignments for a candidate for aldA in Chlorobium limicola DSM 245

Align bifunctional L-rhamnulose 1-phosphate aldolase/lactaldehyde dehydrogenase (EC 4.1.2.19; EC 1.2.1.22) (characterized)
to candidate WP_012466873.1 CLIM_RS09915 bifunctional aldolase/short-chain dehydrogenase

Query= metacyc::BSU31220-MONOMER
         (689 letters)



>NCBI__GCF_000020465.1:WP_012466873.1
          Length = 705

 Score =  346 bits (888), Expect = 2e-99
 Identities = 226/716 (31%), Positives = 366/716 (51%), Gaps = 67/716 (9%)

Query: 11  AAQLPKGVEELVYRSNLIGSDRTVCNWGGGNTSMKTTEKDFRGREIEVMWVKGSGSDLAT 70
           A  +P  + ELVY S L+G + ++   GGGNTS+K+   D  G  + V+++KGSG DLA 
Sbjct: 20  ADDIPAELAELVYASRLLGRESSLVMHGGGNTSVKSELHDIIGNRVNVIFIKGSGVDLAA 79

Query: 71  MKAHNFSGLKLDDIRPL----IKRDQMPDEEM---------------VDYLSHCMIDSKH 111
           + AH+F+ ++++ ++ L       ++  +E+M               + YL+  M+++  
Sbjct: 80  LDAHDFTPVRIEPLQKLQHLYATGERRSEEDMQRFSTREFKNFLYLNLFYLTDHMVNNSL 139

Query: 112 PRPSIETLLHAFLPYKHVDHTHPDAIISICCADNGKQIAEDIYGNRFVWVPYVRPGFTLS 171
             PSIETLLHAFLP++ + HTH  A++++    NG ++  ++ G  F  VPY++PG  L+
Sbjct: 140 S-PSIETLLHAFLPHRFIFHTHSTALLTLSNQPNGAELCREVLGEEFGLVPYIKPGLGLA 198

Query: 172 KMIAEGVANNPHAELVLMEKHGLVTWGETSETCYQKTISIIQEAEQYINDRINQHEVFGG 231
           +  AE   N P    ++++KHGLVT  +++   Y   I  + + E+ I           G
Sbjct: 199 RSAAEAYGNAPDIRGLVLQKHGLVTLADSAAAAYDCMIECVSKLEERI--------ARAG 250

Query: 232 KRYQP---LPEDKRKQILAGIMPVIRGAVSEEK--------KMILSYDDHDDVLEFVNSV 280
           +   P   LPE     +L  + PVIRGAV EEK        + +L +    D+L++VN +
Sbjct: 251 RNVFPSISLPE--TVALLEDVAPVIRGAVVEEKSPGTFEYNQFVLDFRSSPDILQYVNGI 308

Query: 281 QAPALSQIGAACPDHLVHTKRVPLYIDWNPETQDVHKLADLIKSGVETFTSEYQAYFTRN 340
           +   +S  GA  PD ++ TK  PL +   P+  D       +   VE + +EY AYF R 
Sbjct: 309 ELEEVSGRGAMTPDFIIRTKNRPLVVP-APDALDPEGFKSAVHEAVERYKAEYLAYFQRQ 367

Query: 341 QQ-DGDQI--FESAPRVILIPGIGMVNTGKSYAMSKVSGALYRRAIAVMKGATALGQFVS 397
           QQ  G Q+   +  PRV+L+PG+G+   G++   + V+  +     + +  A ++G F S
Sbjct: 368 QQASGMQVTMLDPLPRVVLVPGLGLFGLGRTAHAASVNADIAESTASAILDAQSVGTFES 427

Query: 398 LHENESYHVEYWPLELYKLTLAPPEAEFSRKVALITGGAGGIGSAACRRFAAEGGHVIVA 457
           + E + +++EYW +E  K+     +  F+ KVAL+TG A GIG A  + F   G  +++ 
Sbjct: 428 ITERDVFNIEYWEMEQAKMKKVRHDV-FAGKVALVTGAASGIGLATAKAFRQRGAELVIV 486

Query: 458 DLNIEGAQKIAGEINDAYGKGRAMAVKMDVTKEEDVQSAFERAALAYGGIDIVVNNAGLA 517
           DLN E  ++ + E+      G  +++  DVT    V+ AF+     +GG+DI+V+N G+A
Sbjct: 487 DLNPEALERASAELG-----GGVLSIACDVTDRNAVKRAFDAVCRRFGGLDILVSNVGVA 541

Query: 518 TSSPF----DETSLKEWNLNMNVLGTGYFLVAREAFKQMKHQNRGGSMVFVGSKNSVYAG 573
                    DE   + + LN       +  +A++A + MK Q  GG ++F  SK +V  G
Sbjct: 542 LQGRIGDVADEVLRRSFELNF----FSHQSIAQQAVRIMKLQGTGGVLLFNVSKQAVNPG 597

Query: 574 KNASAYSSVKALETHLARCIAAEGGEFGIRVNSVLPDAVLQGSAIWGSSWREERAAAYGI 633
            +   Y   KA    L R  A + G  GIR N +  D +  G  +      + R+ A G+
Sbjct: 598 PDFGPYGLPKAATMFLVRQYALDHGRDGIRANGINADRIRTG--LLTDEMIKTRSKARGL 655

Query: 634 EPDQLEEHYRKRTALLVNIYPEDIAEAIAFFASSKAEKTTGCMITVDGGVPAAFTR 689
                E  Y     L V +  ED+AE  AF   +   KTTG ++TVDGG  AA  R
Sbjct: 656 S----EREYMAGNLLQVEVTAEDVAE--AFVHQALETKTTGSIVTVDGGNIAAALR 705


Lambda     K      H
   0.317    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1017
Number of extensions: 46
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 689
Length of database: 705
Length adjustment: 39
Effective length of query: 650
Effective length of database: 666
Effective search space:   432900
Effective search space used:   432900
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources