Alignments for a candidate for galE in Geobacter lovleyi SZ

GapMind for catabolism of small carbon sources

Alignments for a candidate for galE in Geobacter lovleyi SZ

Align UDP-glucose 4-epimerase; EC 5.1.3.2; Galactowaldenase; UDP-galactose 4-epimerase (uncharacterized)
to candidate WP_012468955.1 GLOV_RS04315 UDP-glucose 4-epimerase GalE

Query= curated2:Q59083
         (348 letters)



>NCBI__GCF_000020385.1:WP_012468955.1
          Length = 330

 Score =  338 bits (868), Expect = 9e-98
 Identities = 174/324 (53%), Positives = 219/324 (67%)

Query: 8   SPRVLVTGGAGYIGSHVLHALTDAGIPAVTIDDLSAGRREAIPAAVPLVEGDIGSAELLD 67
           S ++LVTGG GYIGSHV+  L++AG   V  D+LS G ++A+     L++ ++     L+
Sbjct: 2   SDKILVTGGCGYIGSHVVRQLSEAGHSVVVYDNLSTGFQDALLHGEELIQAELADHAALE 61

Query: 68  RVMRDHRVDAVMHFAGSIVVPESVVKPLDYYRNNTANSLTLLGACLRAGIDKVVFSSTAA 127
              + HR   V+HFA +IV PESV  PL YY NNT N+L LL A +R G+++ VFSSTAA
Sbjct: 62  AAFQKHRFKTVLHFAAAIVAPESVSLPLKYYGNNTRNTLGLLEASVRNGVERFVFSSTAA 121

Query: 128 VYGAPESVPIREDAPTVPINPYGASKLMTEQMLRDAGAAHGLRSVILRYFNVAGADPAGR 187
           VYG PE     E+    PINPYG SKLM+E MLRD  AAHGL+ V LRYFNVAGADP  R
Sbjct: 122 VYGFPEGGSASEETLLAPINPYGTSKLMSEWMLRDTAAAHGLKYVALRYFNVAGADPQAR 181

Query: 188 TGQATPVATHLIKVACQALLGRRPPLAIFGTDYDTPDGTCIRDYIHVSDLADAHVLALLH 247
            GQ TP ATHLIK+ACQ  LG+R   AI+GTDY TPDGT IRDYIH+ DLA AH+ AL +
Sbjct: 182 IGQRTPEATHLIKIACQTALGQRQQAAIYGTDYPTPDGTGIRDYIHIEDLAAAHLAALDY 241

Query: 248 LRRGGGSLLMNCGYGRGASVREVVRTLEEVSGEQVPATFADRRPGDPPQLVAGADRIREQ 307
           L +GG S  +N GYG+G+SVREV+  +++VSG         RRPGDP  LVA A++I+  
Sbjct: 242 LDKGGESTAINVGYGQGSSVREVLAMVKQVSGVDFRVVEEGRRPGDPACLVARAEKIKLL 301

Query: 308 LGWVPKHDRLDGIVRSALSWERSL 331
             W P+ + L+ IV  A  WE SL
Sbjct: 302 TDWQPRFNSLETIVEDAWRWESSL 325


Lambda     K      H
   0.319    0.136    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 372
Number of extensions: 16
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 348
Length of database: 330
Length adjustment: 28
Effective length of query: 320
Effective length of database: 302
Effective search space:    96640
Effective search space used:    96640
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 49 (23.5 bits)

Align candidate WP_012468955.1 GLOV_RS04315 (UDP-glucose 4-epimerase GalE)
to HMM TIGR01179 (galE: UDP-glucose 4-epimerase GalE (EC 5.1.3.2))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR01179.hmm
# target sequence database:        /tmp/gapView.24770.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR01179  [M=332]
Accession:   TIGR01179
Description: galE: UDP-glucose 4-epimerase GalE
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                                 Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                                 -----------
   1.3e-140  453.9   0.0   1.6e-140  453.6   0.0    1.0  1  lcl|NCBI__GCF_000020385.1:WP_012468955.1  GLOV_RS04315 UDP-glucose 4-epime


Domain annotation for each sequence (and alignments):
>> lcl|NCBI__GCF_000020385.1:WP_012468955.1  GLOV_RS04315 UDP-glucose 4-epimerase GalE
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  453.6   0.0  1.6e-140  1.6e-140       1     328 [.       4     325 ..       4     328 .. 0.99

  Alignments for each domain:
  == domain 1  score: 453.6 bits;  conditional E-value: 1.6e-140
                                 TIGR01179   1 kiLvtGgaGyiGshvvrqllekgkevvvlDnlskgskealkalekitevklvegdladkekleavleee 69 
                                               kiLvtGg+GyiGshvvrql+e+g++vvv+Dnls+g+++al ++e     +l++++lad+++lea+++++
  lcl|NCBI__GCF_000020385.1:WP_012468955.1   4 KILVTGGCGYIGSHVVRQLSEAGHSVVVYDNLSTGFQDALLHGE-----ELIQAELADHAALEAAFQKH 67 
                                               79******************************************.....5******************* PP

                                 TIGR01179  70 kidaviHfaaliavgEsvkePlkYYennvvntleLleamqkagvkkliFsssaavYgesekvpisEesp 138
                                               ++++v+Hfaa i  +Esv+ PlkYY nn+ ntl Llea ++ gv++++Fss+aavYg +e  + sEe+ 
  lcl|NCBI__GCF_000020385.1:WP_012468955.1  68 RFKTVLHFAAAIVAPESVSLPLKYYGNNTRNTLGLLEASVRNGVERFVFSSTAAVYGFPEGGSASEETL 136
                                               ********************************************************************* PP

                                 TIGR01179 139 lnpinpYGrsklmvErilkdlkkadkelkvviLRYFnvaGAdeegeiGeasknathliklvaevavgkr 207
                                               l+pinpYG+sklm+E++l+d++ a ++lk+v+LRYFnvaGAd++ +iG+++++athlik+++++a+g+r
  lcl|NCBI__GCF_000020385.1:WP_012468955.1 137 LAPINPYGTSKLMSEWMLRDTAAA-HGLKYVALRYFNVAGADPQARIGQRTPEATHLIKIACQTALGQR 204
                                               *********************999.******************************************** PP

                                 TIGR01179 208 ekleifGtdyptkDGtcvRDyiHveDlaeaHlaalealeeggesevynlGagqgfsvkevieavkkvsg 276
                                               ++ +i+Gtdypt+DGt++RDyiH+eDla+aHlaal++l +gges++ n+G+gqg sv+ev+  vk+vsg
  lcl|NCBI__GCF_000020385.1:WP_012468955.1 205 QQAAIYGTDYPTPDGTGIRDYIHIEDLAAAHLAALDYLDKGGESTAINVGYGQGSSVREVLAMVKQVSG 273
                                               ********************************************************************* PP

                                 TIGR01179 277 kdikveladrRaGDpaslvadaskikrelgwkpkyddLeeiiksawdWekkl 328
                                               +d++v ++ rR+GDpa+lva a+kik  ++w+p+++ Le+i+++aw+We++l
  lcl|NCBI__GCF_000020385.1:WP_012468955.1 274 VDFRVVEEGRRPGDPACLVARAEKIKLLTDWQPRFNSLETIVEDAWRWESSL 325
                                               *************************************************975 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (332 nodes)
Target sequences:                          1  (330 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.01u 0.00s 00:00:00.01 Elapsed: 00:00:00.01
# Mc/sec: 8.40
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources