Alignments for a candidate for rhaD in Chlorobaculum parvum NCIB 8327

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaD in Chlorobaculum parvum NCIB 8327

Align bifunctional L-rhamnulose 1-phosphate aldolase/lactaldehyde dehydrogenase (EC 4.1.2.19; EC 1.2.1.22) (characterized)
to candidate WP_012501687.1 CPAR_RS02225 bifunctional aldolase/short-chain dehydrogenase

Query= metacyc::BSU31220-MONOMER
         (689 letters)



>NCBI__GCF_000020505.1:WP_012501687.1
          Length = 706

 Score =  337 bits (865), Expect = 9e-97
 Identities = 222/708 (31%), Positives = 361/708 (50%), Gaps = 50/708 (7%)

Query: 11  AAQLPKGVEELVYRSNLIGSDRTVCNWGGGNTSMKTTEKDFRGREIEVMWVKGSGSDLAT 70
           AA  P+ + +LVY S L+G D  +   GGGNTS+K+   DF G ++ V+++K SG +LA+
Sbjct: 20  AADTPEELVDLVYASRLLGKDDRLVMHGGGNTSVKSVLTDFIGNKVNVIFIKASGVNLAS 79

Query: 71  MKAHNFSGLKLDDIRPL--------------IKRDQMPDEEMVDYLSHCMID----SKHP 112
           + A +F+ ++LD +R L              IKR    + +   YL+   +     S+  
Sbjct: 80  VDAGDFTPVRLDPLRKLQQMYASGQRHSEDDIKRFSTREFKNFLYLNLFTLTDHMVSRSL 139

Query: 113 RPSIETLLHAFLPYKHVDHTHPDAIISICCADNGKQIAEDIYGNRFVWVPYVRPGFTLSK 172
            PSIETLLHAFLP++++ HTH  A++++     G ++  +  G+ +  VPY++PG  L+K
Sbjct: 140 SPSIETLLHAFLPHRYILHTHSLALLTLSNQTEGDRMCREALGDGYGMVPYIQPGLGLAK 199

Query: 173 MIAEGVANNPHAELVLMEKHGLVTWGETSETCYQKTISIIQEAEQYINDRINQHEVFGGK 232
              E    NP  E +++ KHGLVT+G++++  Y + I  +   E+ I+    + +VF   
Sbjct: 200 SAFEAYEENPSIEGMVLHKHGLVTFGDSAKEAYDRMIDGVNRIEERISSA--ERKVFAS- 256

Query: 233 RYQPLPEDKRKQILAGIMPVIRGAVSEEK--------KMILSYDDHDDVLEFVNSVQAPA 284
               +P       +  + P++RGA S EK          +L + +   +L+++       
Sbjct: 257 ----VPMPASIATVEQVAPIVRGACSFEKTPGEKDYQSFVLEFRNSPALLDYLKIADLET 312

Query: 285 LSQIGAACPDHLVHTKRVPLYIDWNPETQDVHKLADLIKSGVETFTSEYQAYFTRNQQDG 344
            ++ GA  PD ++ TK  PL +   P+  D+      ++  VE +  EY+AY+ R QQ  
Sbjct: 313 FARKGAMTPDFIIRTKNHPL-VAPAPDAADLEGFGKALRERVERYVEEYRAYYERQQQAT 371

Query: 345 D---QIFESAPRVILIPGIGMVNTGKSYAMSKVSGALYRRAIAVMKGATALGQFVSLHEN 401
                + +  PRV+L+PG+G+   G S   +K++  +   +   M  A ++G F S+ E 
Sbjct: 372 GMEVSMIDPMPRVVLVPGLGLFGLGLSAVDAKLTADIAEHSALAMLDAESIGCFESISER 431

Query: 402 ESYHVEYWPLELYKLTLAPPEAEFSRKVALITGGAGGIGSAACRRFAAEGGHVIVADLNI 461
           +++ +EYW +E  K+       EF+ KV ++TGGAG IG A  + F  +G  +++ DL+ 
Sbjct: 432 DAFEIEYWDMEQAKVRKNRNGGEFAGKVVMVTGGAGAIGLATAKAFKQKGAELVIMDLDP 491

Query: 462 EGAQKIAGEINDAYGKGRAMAVKMDVTKEEDVQSAFERAALAYGGIDIVVNNAGLATSSP 521
               K A E+    G G  +A+  DVT    V+SAF+     YGGIDI+V+N G+A    
Sbjct: 492 TALNKAAEEL----GAG-TLAIPCDVTDASVVRSAFDTICRTYGGIDIIVSNVGVALQGR 546

Query: 522 FDETSLKEWNLNMNVLGTGYFLVAREAFKQMKHQNRGGSMVFVGSKNSVYAGKNASAYSS 581
             + S +    +  +    +  VA+ A K M+ Q  GGS++F  SK +V  G +   Y  
Sbjct: 547 IGDVSDELLRKSFEINFFSHQTVAQNAVKIMRAQKIGGSLLFNVSKQAVNPGADFGPYGL 606

Query: 582 VKALETHLARCIAAEGGEFGIRVNSVLPDAVLQGSAIWGSSWREERAAAYGIEPDQLEEH 641
            KA    L R  A + G  GIR N V  D +  G  +      + R+AA G+     E+ 
Sbjct: 607 PKAATLFLVRQYALDHGRDGIRANGVNADRIRSG--LLTPEMIKARSAARGLS----EKD 660

Query: 642 YRKRTALLVNIYPEDIAEAIAFFASSKAEKTTGCMITVDGGVPAAFTR 689
           Y     L + +  ED+A+A    A     KTTG + TVDGG  AA  R
Sbjct: 661 YMAGNLLGLEVTAEDVADAFVHLALE--AKTTGSITTVDGGNIAAALR 706


Lambda     K      H
   0.317    0.134    0.396 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 974
Number of extensions: 44
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 689
Length of database: 706
Length adjustment: 39
Effective length of query: 650
Effective length of database: 667
Effective search space:   433550
Effective search space used:   433550
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources